Development of a Multiplex TaqMan Assay for Rapid Detection of Groundnut Bud Necrosis Virus: A Quarantine Pathogen in the USA

Development of a Multiplex TaqMan Assay for Rapid Detection of Groundnut Bud Necrosis Virus: A Quarantine Pathogen in the USA

Groundnut bud necrosis orthotospovirus (GBNV), a tripartite single-stranded RNA virus, poses a significant threat to United States agriculture. GBNV is a quarantine pathogen, and its introduction could lead to severe damage to economically important crops, such as groundnuts, tomatoes, potatoes, pea...

Full description

Saved in:

Bibliographic Details
Main Authors:	Anushi Suwaneththiya Deraniyagala, Avijit Roy, Shyam Tallury, Hari Kishan Sudini, Albert K. Culbreath, Sudeep Bag
Format:	Article
Language:	English
Published:	MDPI AG 2025-04-01
Series:	Viruses
Subjects:	groundnut bud necrosis orthotospovirus (GBNV) <i>Orthotospovirus arachinecrosis</i> phytosanitary measures TaqMan RT-qPCR quarantine pathogen
Online Access:	https://www.mdpi.com/1999-4915/17/4/532
Tags:	Add Tag No Tags, Be the first to tag this record!

Similar Items

A TaqMan qPCR for precise detection and quantification of diarrheagenic Escherichia coli
by: Bingyu Xue, et al.
Published: (2025-05-01)

Establishment of a TaqMan Quantitative Real-Time PCR for Detecting <i>Lawsonia intracellularis</i>
by: Zhiqiang Hu, et al.
Published: (2025-05-01)

Specific detection of pigeon parvovirus with TaqMan real-time PCR technology
by: Cuiteng Chen, et al.
Published: (2025-01-01)

Application of TaqMan low density array to simultaneous identification of multiple animal-derived components
by: YU Huizhen, et al.
Published: (2020-04-01)

Dehydration and tomato spotted wilt virus infection combine to alter feeding and survival parameters for the western flower thrips, Frankliniella occidentalis
by: Samuel T. Bailey, et al.
Published: (2024-01-01)

A novel TaqMan qPCR system for the detection of Diaporthe citri, the causative agent of citrus melanose
by: Zhanxu Pu, et al.
Published: (2024-12-01)

Development of a TaqMan qPCR for the Simultaneous Detection of the TuMV and BBWV2 Viruses Responsible for the Viral Disease in <i>Pseudostellaria heterophylla</i>
by: Li Gu, et al.
Published: (2024-12-01)

Screening of tuberculosis suspected subjects using real-time PCR, TaqMan method; Northeastern Iran
by: Sanaz Ahmadi Ghezeldasht, et al.
Published: (2025-11-01)

Specific detection of duck adeno-associated virus using a TaqMan-based real-time PCR assay
by: Shuyu Chen, et al.
Published: (2024-11-01)

Analysis for Bopivirus B in goats in the Sichuan province, China using a novel TaqMan real-time polymerase chain reaction assay
by: Kehamo Abi, et al.
Published: (2025-03-01)

Development and application of a TaqMan-MGB probe-based quantitative real-time polymerase chain reaction assay for the rapid detection of Dictyocaulus filaria
by: Zheng-qin Gao, et al.
Published: (2025-08-01)

Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens
by: Hao Wang, et al.
Published: (2025-12-01)

Establishment of Singleplex and Duplex TaqMan RT-qPCR Detection Systems for Strawberry Mottle Virus (SMoV) and Strawberry Vein Banding Virus (SVBV)
by: Tengfei Xu, et al.
Published: (2025-07-01)

Tomato spotted wilt virus detection in the potato cultivar Innovator
by: A. E. Salvalaggio, et al.
Published: (2024-01-01)

The quadruplex TaqMan MGB fluorescent quantitative PCR method for simultaneous detection of feline panleukopenia virus, feline herpesvirus 1, feline calicivirus and feline infectious peritonitis virus
by: Haojie Wang, et al.
Published: (2025-05-01)

Rapid Identification of the SNP Mutation in the <i>ABCD4</i> Gene and Its Association with Multi-Vertebrae Phenotypes in Ujimqin Sheep Using TaqMan-MGB Technology
by: Yue Zhang, et al.
Published: (2025-08-01)

Development of a TaqMan-based quantitative real-time PCR for the detection of Porcine circovirus type 3
by: Zhiqiang Hu, et al.
Published: (2025-07-01)

Establishment of a TaqMan qPCR method with MGB probe for the specific detection of BVDV field strains circulating in China
by: Lele An, et al.
Published: (2025-07-01)

Use of a TaqMan Array Card for identification of enterotoxins and colonization factors directly from stool samples in an enterotoxigenic E. coli vaccine study
by: Jie Liu, et al.
Published: (2025-03-01)

Development and Application of a TaqMan RT-qPCR for the Detection of Foot-and-Mouth Disease Virus in Pigs
by: Changying Dong, et al.
Published: (2024-11-01)

New molecular diagnostic and surveillance tools for orthotospoviruses and their thrips vectors
by: Hsu-Yao Chao, et al.
Published: (2025-05-01)

A developed TaqMan probe-based qPCR was used to quantify the distribution of AMDV in various tissues of infected mink and its prevalence in northern China
by: Zaixing Yang, et al.
Published: (2025-01-01)

Research note: Simultaneous detection of GPV, H5 AIV, and GoAstV via TaqMan probe-based multiplex qPCR
by: Xiaoquan Wang, et al.
Published: (2024-12-01)

Evaluation of spider lilies (Hymenocallis spp.) as sentinel plants for orthotospoviruses
by: Hsu-Yao Chao, et al.
Published: (2025-04-01)

Development of Multiple Real-Time Fluorescent Quantitative PCR for <i>Vibrio</i> Pathogen Detection in Aquaculture
by: Binzhe Zhang, et al.
Published: (2025-04-01)

Highly sensitive scanning of gene mutations: TaqMan probes as blocking agents
by: I. V. Botezatu, et al.
Published: (2016-06-01)

Development and Application of a Multiplex Real-Time TaqMan qPCR Assay for the Simultaneous Detection of African Swine Fever Virus, Classical Swine Fever Virus, Porcine Reproductive and Respiratory Syndrome Virus, Pseudorabies Virus, and Porcine Circovirus Type 2
by: Dongdong Yin, et al.
Published: (2025-07-01)

Suitability Evaluation of Mudstone of Injana Formation for Dam Filling Materials in TaqTaq area/Erbil/Iraq
by: Fahad Jamal Suhael, et al.
Published: (2020-03-01)

Changing Scenario of Groundnut Stem and Collar Rot Diseases in Major Groundnut Growing Areas of Telangana
by: J. Chandrakala, et al.
Published: (2024-05-01)

Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome
by: Zhifei Zhang, et al.
Published: (2025-03-01)

Validation of Prognostic Circulating Cell-Free RNA Biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> in Colorectal Cancer Through TaqMan qPCR and Correlation Analysis
by: Chau Ming Kan, et al.
Published: (2025-07-01)

Development of TaqMan-Based Real-Time qPCR Method for Accurate Detection and Quantification of Citrus Psorosis Virus and Cytoplasmic-Type Citrus Leprosis Virus in Saplings
by: Minhue Jung, et al.
Published: (2024-12-01)

Establishment and Application of TaqMan qPCR Detection Method for Human DNA Contamination in DNA Laboratory
by: SHEN Gao-fang, et al.
Published: (2025-02-01)

Arising amitraz and pyrethroids resistance mutations in the ectoparasitic Varroa destructor mite in Canada
by: Rassol Bahreini, et al.
Published: (2025-01-01)

High throughput screening for human disease associated-pathogens and antimicrobial resistance genes in migratory birds at ten habitat sites in China
by: Lan Wang, et al.
Published: (2025-06-01)

Off-grid field-deployable molecular diagnostic platform for malaria surveillance
by: Madhavinadha Prasad Kona, et al.
Published: (2025-04-01)

An Optimized Probe‐Based qPCR Assay for the Detection and Monitoring of the Invasive Lionfish (Pterois volitans) in the Atlantic
by: Katherine Viehl, et al.
Published: (2025-03-01)

Discrimination of SNPs in GC-rich regions using a modified hydrolysis probe chemistry protocol
by: Alireza Mowjoodi, et al.
Published: (2014-12-01)

Association of Sheep Tail Type with the T Gene Single Nucleotide Polymorphisms Loci
by: Daqing Wang, et al.
Published: (2025-02-01)

Economic Analysis of Groundnut Production in Andhra Pradesh
by: G. P. Sunandini, et al.
Published: (2020-11-01)