Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons
Cholinergic mechanism is involved in motor behavior. In songbirds, the robust nucleus of the arcopallium (RA) is a song premotor nucleus in the pallium and receives cholinergic inputs from the basal forebrain. The activity of projection neurons in RA determines song motor behavior. Although many evi...
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| Format: | Article |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/7246827 |
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| author | Wei Meng Song-Hua Wang Dong-Feng Li |
| author_facet | Wei Meng Song-Hua Wang Dong-Feng Li |
| author_sort | Wei Meng |
| collection | DOAJ |
| description | Cholinergic mechanism is involved in motor behavior. In songbirds, the robust nucleus of the arcopallium (RA) is a song premotor nucleus in the pallium and receives cholinergic inputs from the basal forebrain. The activity of projection neurons in RA determines song motor behavior. Although many evidences suggest that cholinergic system is implicated in song production, the cholinergic modulation of RA is not clear until now. In the present study, the electrophysiological effects of carbachol, a nonselective cholinergic receptor agonist, were investigated on the RA projection neurons of adult male zebra finches through whole-cell patch-clamp techniques in vitro. Our results show that carbachol produced a significant decrease in the spontaneous and evoked action potential (AP) firing frequency of RA projection neurons, accompanying a hyperpolarization of the membrane potential, an increase in the evoked AP latency, afterhyperpolarization (AHP) peak amplitude, and AHP time to peak, and a decrease in the membrane input resistance, membrane time constant, and membrane capacitance. These results indicate that carbachol reduces the activity of RA projection neurons by hyperpolarizing the resting membrane potential and increasing the AHP and the membrane conductance, suggesting that the cholinergic modulation of RA may play an important role in song production. |
| format | Article |
| id | doaj-art-99cd856afea4459a96cafb50a5c07811 |
| institution | OA Journals |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-99cd856afea4459a96cafb50a5c078112025-08-20T02:20:15ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/72468277246827Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection NeuronsWei Meng0Song-Hua Wang1Dong-Feng Li2School of Life Science, South China Normal University, Guangzhou, Guangdong 510631, ChinaSchool of Life Science, South China Normal University, Guangzhou, Guangdong 510631, ChinaSchool of Life Science, South China Normal University, Guangzhou, Guangdong 510631, ChinaCholinergic mechanism is involved in motor behavior. In songbirds, the robust nucleus of the arcopallium (RA) is a song premotor nucleus in the pallium and receives cholinergic inputs from the basal forebrain. The activity of projection neurons in RA determines song motor behavior. Although many evidences suggest that cholinergic system is implicated in song production, the cholinergic modulation of RA is not clear until now. In the present study, the electrophysiological effects of carbachol, a nonselective cholinergic receptor agonist, were investigated on the RA projection neurons of adult male zebra finches through whole-cell patch-clamp techniques in vitro. Our results show that carbachol produced a significant decrease in the spontaneous and evoked action potential (AP) firing frequency of RA projection neurons, accompanying a hyperpolarization of the membrane potential, an increase in the evoked AP latency, afterhyperpolarization (AHP) peak amplitude, and AHP time to peak, and a decrease in the membrane input resistance, membrane time constant, and membrane capacitance. These results indicate that carbachol reduces the activity of RA projection neurons by hyperpolarizing the resting membrane potential and increasing the AHP and the membrane conductance, suggesting that the cholinergic modulation of RA may play an important role in song production.http://dx.doi.org/10.1155/2016/7246827 |
| spellingShingle | Wei Meng Song-Hua Wang Dong-Feng Li Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons Neural Plasticity |
| title | Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons |
| title_full | Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons |
| title_fullStr | Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons |
| title_full_unstemmed | Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons |
| title_short | Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons |
| title_sort | carbachol induced reduction in the activity of adult male zebra finch ra projection neurons |
| url | http://dx.doi.org/10.1155/2016/7246827 |
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