Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA Fragmentation
Background: This study aims to compare the clinical pregnancy outcome of intracytoplasmic sperm injection (ICSI) using testicular and ejaculated spermatozoa in male patients with high deoxyribonucleic acid (DNA) fragmentation. Patients diagnosed with high DNA fragmentation levels in sperm DNA fragme...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-05-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_313_25 |
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| author | Juhi Barai Akash More Namrata Choudhary |
| author_facet | Juhi Barai Akash More Namrata Choudhary |
| author_sort | Juhi Barai |
| collection | DOAJ |
| description | Background:
This study aims to compare the clinical pregnancy outcome of intracytoplasmic sperm injection (ICSI) using testicular and ejaculated spermatozoa in male patients with high deoxyribonucleic acid (DNA) fragmentation. Patients diagnosed with high DNA fragmentation levels in sperm DNA fragmentation (SDF) tests using sperm chromatin dispersion (SCD) are categorized into two groups one including the male patient undergoing ICSI with ejaculated and another with testicular spermatozoa retrieved via testicular sperm aspiration (TESA). The study evaluates the clinical outcome including fertilization, implantation, and pregnancy rates, comparing the efficacy of testicular versus ejaculated sperm in overcoming the adverse effects of DNA fragmentation. By analyzing ICSI outcomes, the study seeks to establish standardized SDF testing protocols.
Objective:
The study aims to identify male partners with abnormal semen parameters and evaluate sperm DNA fragmentation (SDF) in both ejaculated and testicular sperm. Additionally, it seeks to assess the effectiveness of the SDF test in detecting DNA damage. Furthermore, the study compares the clinical pregnancy outcomes of ICSI using testicular and ejaculated spermatozoa in male patients with high DNA fragmentation.
Methodology:
The methodology involves patient consultation, consent, and screening for infections. Semen analysis and DNA fragmentation tests assess sperm quality. For azoospermic patients with high SDF, TESA retrieves sperm for ICSI and normal from ejaculate with high SDF. Outcomes are evaluated for treatment success and DNA integrity.
Expected Results:
Standardization of the SDF test, in which the best SDF test will be adopted, and a precise threshold will be used to determine the sperm DNA damage which may enhance the ICSI outcome, along with the difference between the ICSI outcome of both the testicular and ejaculated spermatozoa with high SDF.
Study Implication:
It helps in assessing the ICSI outcome in testicular and ejaculated spermatozoa in patients with high DNA fragmentation. |
| format | Article |
| id | doaj-art-99bca20889a54b3ebfdc36ba4e9e84e5 |
| institution | OA Journals |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-99bca20889a54b3ebfdc36ba4e9e84e52025-08-20T02:34:43ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062025-05-0117Suppl 1S936S93910.4103/jpbs.jpbs_313_25Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA FragmentationJuhi BaraiAkash MoreNamrata ChoudharyBackground: This study aims to compare the clinical pregnancy outcome of intracytoplasmic sperm injection (ICSI) using testicular and ejaculated spermatozoa in male patients with high deoxyribonucleic acid (DNA) fragmentation. Patients diagnosed with high DNA fragmentation levels in sperm DNA fragmentation (SDF) tests using sperm chromatin dispersion (SCD) are categorized into two groups one including the male patient undergoing ICSI with ejaculated and another with testicular spermatozoa retrieved via testicular sperm aspiration (TESA). The study evaluates the clinical outcome including fertilization, implantation, and pregnancy rates, comparing the efficacy of testicular versus ejaculated sperm in overcoming the adverse effects of DNA fragmentation. By analyzing ICSI outcomes, the study seeks to establish standardized SDF testing protocols. Objective: The study aims to identify male partners with abnormal semen parameters and evaluate sperm DNA fragmentation (SDF) in both ejaculated and testicular sperm. Additionally, it seeks to assess the effectiveness of the SDF test in detecting DNA damage. Furthermore, the study compares the clinical pregnancy outcomes of ICSI using testicular and ejaculated spermatozoa in male patients with high DNA fragmentation. Methodology: The methodology involves patient consultation, consent, and screening for infections. Semen analysis and DNA fragmentation tests assess sperm quality. For azoospermic patients with high SDF, TESA retrieves sperm for ICSI and normal from ejaculate with high SDF. Outcomes are evaluated for treatment success and DNA integrity. Expected Results: Standardization of the SDF test, in which the best SDF test will be adopted, and a precise threshold will be used to determine the sperm DNA damage which may enhance the ICSI outcome, along with the difference between the ICSI outcome of both the testicular and ejaculated spermatozoa with high SDF. Study Implication: It helps in assessing the ICSI outcome in testicular and ejaculated spermatozoa in patients with high DNA fragmentation.https://journals.lww.com/10.4103/jpbs.jpbs_313_25dna fragmentationinfertilityintracytoplasmic sperm injection (icsi)tesa |
| spellingShingle | Juhi Barai Akash More Namrata Choudhary Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA Fragmentation Journal of Pharmacy and Bioallied Sciences dna fragmentation infertility intracytoplasmic sperm injection (icsi) tesa |
| title | Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA Fragmentation |
| title_full | Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA Fragmentation |
| title_fullStr | Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA Fragmentation |
| title_full_unstemmed | Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA Fragmentation |
| title_short | Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA Fragmentation |
| title_sort | study protocol comparison of clinical pregnancy outcomes of intracytoplasmic sperm injection icsi using testicular and ejaculated spermatozoa in male patients with high dna fragmentation |
| topic | dna fragmentation infertility intracytoplasmic sperm injection (icsi) tesa |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_313_25 |
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