Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants
Summary: Here, we present a protocol for a mouse model for studying mechanisms of post-primary pulmonary tuberculosis (PTB) caused by virulent Mycobacterium tuberculosis (Mtb) using subcutaneous hock infection and lung tissue implantation. We describe steps for collagen instillation of lungs, lung a...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003909 |
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| author | Shivraj M. Yabaji Suruchi Lata Igor Gavrish Ming Lo Aoife K. O’Connell Hans P. Gertje Colleen E. Thurman Nicholas A. Crossland Lester Kobzik Igor Kramnik |
| author_facet | Shivraj M. Yabaji Suruchi Lata Igor Gavrish Ming Lo Aoife K. O’Connell Hans P. Gertje Colleen E. Thurman Nicholas A. Crossland Lester Kobzik Igor Kramnik |
| author_sort | Shivraj M. Yabaji |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for a mouse model for studying mechanisms of post-primary pulmonary tuberculosis (PTB) caused by virulent Mycobacterium tuberculosis (Mtb) using subcutaneous hock infection and lung tissue implantation. We describe steps for collagen instillation of lungs, lung and spleen implantation, preparation of Mtb for infection, and hock infection of mice. We then detail procedures for the perfusion of the lung and collection of organs, tissue processing, and histopathologic interpretation.For complete details on the use and execution of this protocol, please refer to Yabaji et al.1,2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-99aa0c64a96a418da9b4183e7d14d4ee |
| institution | DOAJ |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-99aa0c64a96a418da9b4183e7d14d4ee2025-08-20T02:47:19ZengElsevierSTAR Protocols2666-16672025-09-016310398410.1016/j.xpro.2025.103984Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implantsShivraj M. Yabaji0Suruchi Lata1Igor Gavrish2Ming Lo3Aoife K. O’Connell4Hans P. Gertje5Colleen E. Thurman6Nicholas A. Crossland7Lester Kobzik8Igor Kramnik9The National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USAThe National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USAThe National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USAThe National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USAThe National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USAThe National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USAThe National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USAThe National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USA; The Department of Pathology and Laboratory Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA 02118, USACellecta, Inc., Mountain View, CA, USAThe National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USA; Pulmonary Center, The Department of Medicine, Boston University Chobanian & Aveedisian School of Medicine, Boston, MA 02118, USA; Corresponding authorSummary: Here, we present a protocol for a mouse model for studying mechanisms of post-primary pulmonary tuberculosis (PTB) caused by virulent Mycobacterium tuberculosis (Mtb) using subcutaneous hock infection and lung tissue implantation. We describe steps for collagen instillation of lungs, lung and spleen implantation, preparation of Mtb for infection, and hock infection of mice. We then detail procedures for the perfusion of the lung and collection of organs, tissue processing, and histopathologic interpretation.For complete details on the use and execution of this protocol, please refer to Yabaji et al.1,2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725003909ImmunologyMicrobiologyModel Organisms |
| spellingShingle | Shivraj M. Yabaji Suruchi Lata Igor Gavrish Ming Lo Aoife K. O’Connell Hans P. Gertje Colleen E. Thurman Nicholas A. Crossland Lester Kobzik Igor Kramnik Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants STAR Protocols Immunology Microbiology Model Organisms |
| title | Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants |
| title_full | Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants |
| title_fullStr | Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants |
| title_full_unstemmed | Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants |
| title_short | Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants |
| title_sort | protocol for developing a mouse model of post primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants |
| topic | Immunology Microbiology Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725003909 |
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