Piper Essential Oils Inhibit Rhizopus oryzae Growth, Biofilm Formation, and Rhizopuspepsin Activity

Piper is the largest genus of the Piperaceae family. The species of this genus have diverse biological activities and are used in pharmacopeia throughout the world. They are also used in folk medicine for treatment of many diseases in several countries including Brazil, China, India, Jamaica, and Me...

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Main Authors: Catia A. Almeida, Mariana M. B. Azevedo, Francisco C. M. Chaves, Marcelo Roseo de Oliveira, Igor A. Rodrigues, Humberto R. Bizzo, Paola E. Gama, Daniela S. Alviano, Celuta S. Alviano
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Canadian Journal of Infectious Diseases and Medical Microbiology
Online Access:http://dx.doi.org/10.1155/2018/5295619
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author Catia A. Almeida
Mariana M. B. Azevedo
Francisco C. M. Chaves
Marcelo Roseo de Oliveira
Igor A. Rodrigues
Humberto R. Bizzo
Paola E. Gama
Daniela S. Alviano
Celuta S. Alviano
author_facet Catia A. Almeida
Mariana M. B. Azevedo
Francisco C. M. Chaves
Marcelo Roseo de Oliveira
Igor A. Rodrigues
Humberto R. Bizzo
Paola E. Gama
Daniela S. Alviano
Celuta S. Alviano
author_sort Catia A. Almeida
collection DOAJ
description Piper is the largest genus of the Piperaceae family. The species of this genus have diverse biological activities and are used in pharmacopeia throughout the world. They are also used in folk medicine for treatment of many diseases in several countries including Brazil, China, India, Jamaica, and Mexico. In Brazil, Piper species are distributed throughout the national territory, making this genus a good candidate for biological activity screening. During our studies with Piper essential oils, we evaluated its activity against Rhizopus oryzae, the main agent of mucormycosis. The main compounds of seven Piper essential oils analyzed were Piper callosum—safrole (53.8%), P. aduncum—dillapiole (76.0%), P. hispidinervum—safrole (91.4%), P. marginatum—propiopiperone (13.2%), P. hispidum—γ-terpinene (30.9%), P. tuberculatum—(E)-caryophyllene (30.1%), and Piper sp.—linalool (14.6%). The minimum inhibitory concentration of Piper essential oils against R. oryzae ranged from 78.12 to >1250 μg/mL. The best result of total inhibition of biofilm formation was obtained with Piper sp. starting from 4.88 μg/mL. Considering the bioactive potential of EOs against planktonic cells and biofilm formation of R. oryzae could be of great interest for development of antimicrobials for therapeutic use in treatment of fungal infection.
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spelling doaj-art-99a06cd0c25b403db3ed6a229ba85a392025-08-20T02:18:25ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932018-01-01201810.1155/2018/52956195295619Piper Essential Oils Inhibit Rhizopus oryzae Growth, Biofilm Formation, and Rhizopuspepsin ActivityCatia A. Almeida0Mariana M. B. Azevedo1Francisco C. M. Chaves2Marcelo Roseo de Oliveira3Igor A. Rodrigues4Humberto R. Bizzo5Paola E. Gama6Daniela S. Alviano7Celuta S. Alviano8Department of General Microbiology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro (IMPG-UFRJ), CCS Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, BrazilDepartment of General Microbiology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro (IMPG-UFRJ), CCS Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, BrazilEMBRAPA Western Amazon, Rodovia AM 10 km 29, 69010-970 Manaus, AM, BrazilEMBRAPA Western Amazon, Rodovia AM 10 km 29, 69010-970 Manaus, AM, BrazilSchool of Farmacy, Department of Natural Products and Food, Federal University of Rio de Janeiro, CCS Ilha do Fundão, 21941-902 Rio de Janeiro, RJ, BrazilEMBRAPA Food Technology, Avenida das Américas 29501, 23020-470 Rio de Janeiro, RJ, BrazilEMBRAPA Food Technology, Avenida das Américas 29501, 23020-470 Rio de Janeiro, RJ, BrazilDepartment of General Microbiology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro (IMPG-UFRJ), CCS Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, BrazilDepartment of General Microbiology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro (IMPG-UFRJ), CCS Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, BrazilPiper is the largest genus of the Piperaceae family. The species of this genus have diverse biological activities and are used in pharmacopeia throughout the world. They are also used in folk medicine for treatment of many diseases in several countries including Brazil, China, India, Jamaica, and Mexico. In Brazil, Piper species are distributed throughout the national territory, making this genus a good candidate for biological activity screening. During our studies with Piper essential oils, we evaluated its activity against Rhizopus oryzae, the main agent of mucormycosis. The main compounds of seven Piper essential oils analyzed were Piper callosum—safrole (53.8%), P. aduncum—dillapiole (76.0%), P. hispidinervum—safrole (91.4%), P. marginatum—propiopiperone (13.2%), P. hispidum—γ-terpinene (30.9%), P. tuberculatum—(E)-caryophyllene (30.1%), and Piper sp.—linalool (14.6%). The minimum inhibitory concentration of Piper essential oils against R. oryzae ranged from 78.12 to >1250 μg/mL. The best result of total inhibition of biofilm formation was obtained with Piper sp. starting from 4.88 μg/mL. Considering the bioactive potential of EOs against planktonic cells and biofilm formation of R. oryzae could be of great interest for development of antimicrobials for therapeutic use in treatment of fungal infection.http://dx.doi.org/10.1155/2018/5295619
spellingShingle Catia A. Almeida
Mariana M. B. Azevedo
Francisco C. M. Chaves
Marcelo Roseo de Oliveira
Igor A. Rodrigues
Humberto R. Bizzo
Paola E. Gama
Daniela S. Alviano
Celuta S. Alviano
Piper Essential Oils Inhibit Rhizopus oryzae Growth, Biofilm Formation, and Rhizopuspepsin Activity
Canadian Journal of Infectious Diseases and Medical Microbiology
title Piper Essential Oils Inhibit Rhizopus oryzae Growth, Biofilm Formation, and Rhizopuspepsin Activity
title_full Piper Essential Oils Inhibit Rhizopus oryzae Growth, Biofilm Formation, and Rhizopuspepsin Activity
title_fullStr Piper Essential Oils Inhibit Rhizopus oryzae Growth, Biofilm Formation, and Rhizopuspepsin Activity
title_full_unstemmed Piper Essential Oils Inhibit Rhizopus oryzae Growth, Biofilm Formation, and Rhizopuspepsin Activity
title_short Piper Essential Oils Inhibit Rhizopus oryzae Growth, Biofilm Formation, and Rhizopuspepsin Activity
title_sort piper essential oils inhibit rhizopus oryzae growth biofilm formation and rhizopuspepsin activity
url http://dx.doi.org/10.1155/2018/5295619
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