Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4
CXC chemokine release can be modulated by Th2-derived cytokines. Interleukin(IL)-4 is one of the cytokines that are the hallmark of the Th-2 response, and plays an important role in human tuberculosis. In the current study, we investigated the effect of IL-4 on chemokine production by human epitheli...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/67451 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832568092641722368 |
|---|---|
| author | Patricia Méndez-Samperio Elena Miranda Abraham Vázquez |
| author_facet | Patricia Méndez-Samperio Elena Miranda Abraham Vázquez |
| author_sort | Patricia Méndez-Samperio |
| collection | DOAJ |
| description | CXC chemokine release can be modulated by Th2-derived
cytokines. Interleukin(IL)-4 is one of the cytokines that are the
hallmark of the Th-2 response, and plays an important role in
human tuberculosis. In the current study, we investigated the
effect of IL-4 on chemokine production by human epithelial cells
infected with Mycobacterium bovis bacillus
calmette-guérin (BCG). Gene expression of CXCL-8 and
CXCL-10 was determined by the reverse transcription
(RT)-polymerase chain reaction method. The levels of
immunoreactive CXCL-8 and CXCL-10 were determined by enzyme-linked
immunosorbent assay. We found that, although M.
bovis BCG induced gene expression of CXCL-8 and CXCL-10 in
M. bovis BCG-infected human epithelial cells, CXCL-8 mRNA
level was significantly reduced by IL-4, whereas no significant
effect of IL-4 was observed on CXCL10 mRNA level. In addition,
IL-4 decreased CXCL-8 (in a graded and significant manner) but not
CXCL-10 secretion. These results were further confirmed, since a
significant reversion was obtained with a neutralizing antibody to
human IL-4, whereas an isotype-matched control antibody had no
significant effect on CXCL-8 secretion. Furthermore, we found a
similar effect of IL-4 on M. bovis BCG-induced CXCL-8 and
CXCL-10 secretion by using other human epithelial A549 cell line.
Collectively, these data demonstrate that M.
bovis BCG-infected human epithelial cells can have an active role
in a local inflammatory immune response via the secretion of CXC
chemokines which can be selectively regulated by Th2-derived
cytokines. |
| format | Article |
| id | doaj-art-999a040fcbdf45bf9c0be13a363e032e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-999a040fcbdf45bf9c0be13a363e032e2025-02-03T00:59:46ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/6745167451Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4Patricia Méndez-Samperio0Elena Miranda1Abraham Vázquez2Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN. Carpio y Plan de Ayala, México D.F. 11340, MexicoDepartamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN. Carpio y Plan de Ayala, México D.F. 11340, MexicoDepartamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN. Carpio y Plan de Ayala, México D.F. 11340, MexicoCXC chemokine release can be modulated by Th2-derived cytokines. Interleukin(IL)-4 is one of the cytokines that are the hallmark of the Th-2 response, and plays an important role in human tuberculosis. In the current study, we investigated the effect of IL-4 on chemokine production by human epithelial cells infected with Mycobacterium bovis bacillus calmette-guérin (BCG). Gene expression of CXCL-8 and CXCL-10 was determined by the reverse transcription (RT)-polymerase chain reaction method. The levels of immunoreactive CXCL-8 and CXCL-10 were determined by enzyme-linked immunosorbent assay. We found that, although M. bovis BCG induced gene expression of CXCL-8 and CXCL-10 in M. bovis BCG-infected human epithelial cells, CXCL-8 mRNA level was significantly reduced by IL-4, whereas no significant effect of IL-4 was observed on CXCL10 mRNA level. In addition, IL-4 decreased CXCL-8 (in a graded and significant manner) but not CXCL-10 secretion. These results were further confirmed, since a significant reversion was obtained with a neutralizing antibody to human IL-4, whereas an isotype-matched control antibody had no significant effect on CXCL-8 secretion. Furthermore, we found a similar effect of IL-4 on M. bovis BCG-induced CXCL-8 and CXCL-10 secretion by using other human epithelial A549 cell line. Collectively, these data demonstrate that M. bovis BCG-infected human epithelial cells can have an active role in a local inflammatory immune response via the secretion of CXC chemokines which can be selectively regulated by Th2-derived cytokines.http://dx.doi.org/10.1155/MI/2006/67451 |
| spellingShingle | Patricia Méndez-Samperio Elena Miranda Abraham Vázquez Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 Mediators of Inflammation |
| title | Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 |
| title_full | Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 |
| title_fullStr | Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 |
| title_full_unstemmed | Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 |
| title_short | Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 |
| title_sort | expression and secretion of cxcl 8 and cxcl 10 from mycobacterium bovis bcg infected human epithelial cells role of il 4 |
| url | http://dx.doi.org/10.1155/MI/2006/67451 |
| work_keys_str_mv | AT patriciamendezsamperio expressionandsecretionofcxcl8andcxcl10frommycobacteriumbovisbcginfectedhumanepithelialcellsroleofil4 AT elenamiranda expressionandsecretionofcxcl8andcxcl10frommycobacteriumbovisbcginfectedhumanepithelialcellsroleofil4 AT abrahamvazquez expressionandsecretionofcxcl8andcxcl10frommycobacteriumbovisbcginfectedhumanepithelialcellsroleofil4 |