Serogroup Prevalence, Virulence Profile and Antibiotic Resistance of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Chicken
Avian pathogenic <i>Escherichia coli</i> (APEC) causes avian colibacillosis, leading to significant economic losses and concerns for food safety in the poultry industry. This study focused on examining the virulence gene profile, antibiotic resistance prevalence, and resistance patterns...
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MDPI AG
2025-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/12/6/592 |
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| author | Showkat A. Shah Masood S. Mir Shayaib A. Kamil Majid Shafi Mudasir A. Rather Azmat A. Khan Zahoor A. Wani Sheikh Adil Fatmah M. Alqahtani Majid Alhomrani Manzoor Wani |
| author_facet | Showkat A. Shah Masood S. Mir Shayaib A. Kamil Majid Shafi Mudasir A. Rather Azmat A. Khan Zahoor A. Wani Sheikh Adil Fatmah M. Alqahtani Majid Alhomrani Manzoor Wani |
| author_sort | Showkat A. Shah |
| collection | DOAJ |
| description | Avian pathogenic <i>Escherichia coli</i> (APEC) causes avian colibacillosis, leading to significant economic losses and concerns for food safety in the poultry industry. This study focused on examining the virulence gene profile, antibiotic resistance prevalence, and resistance patterns of APEC isolates. A total of 250 bacterial strains were collected from birds affected by colibacillosis. Serogrouping revealed diverse serotypes, with O2 being the most common (16%), followed by O1, O8, and O76. All isolates tested positive for at minimum one virulence gene, with 7.2% carrying all five targeted genes, particularly in serogroups O1, O8, O45, and O88. The most detected gene was <i>iss</i>, present in 79.6% of isolates, followed by <i>tsh</i>, <i>iuc</i>C, <i>sitA</i>, and <i>pap</i>C. The antibiotic resistance analysis showed that all isolates exhibited multidrug resistance, although they remained susceptible to gentamicin, amikacin, ciprofloxacin, and chloramphenicol. Moreover, specific antibiotic resistance genes were known in the isolates, with <i>tetA</i> detected in 54.8%, <i>tetB</i> in 51.7%, <i>sul1</i> in 50%, and <i>aadA1</i> in 29.2%. These findings highlight the widespread antibiotic resistance in chicken carcasses, which poses a hazard to human health in terms of transfer of resistance to humans, reduced effectiveness of antibiotics and impaired ability to contain infectious diseases. Therefore, it is crucial to implement strict monitoring programs to regulate antibiotic usage in poultry production. |
| format | Article |
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| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-999708172fca4395834dba455daab3e22025-08-20T03:32:32ZengMDPI AGVeterinary Sciences2306-73812025-06-0112659210.3390/vetsci12060592Serogroup Prevalence, Virulence Profile and Antibiotic Resistance of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler ChickenShowkat A. Shah0Masood S. Mir1Shayaib A. Kamil2Majid Shafi3Mudasir A. Rather4Azmat A. Khan5Zahoor A. Wani6Sheikh Adil7Fatmah M. Alqahtani8Majid Alhomrani9Manzoor Wani10Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama 190006, IndiaDivision of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama 190006, IndiaDivision of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama 190006, IndiaDivision of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama 190006, IndiaDivision of Veterinary Public Health, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama 190006, IndiaDivision of Livestock Production and Management, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama 190006, IndiaDivision of Veterinary Parasitology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama 190006, IndiaDivision of Livestock Production and Management, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama 190006, IndiaDepartment of Biology, College of Science, King Khalid University, Abha 61413, Saudi ArabiaDepartment of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaDivision of Livestock Production and Management, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, Shuhama 190006, IndiaAvian pathogenic <i>Escherichia coli</i> (APEC) causes avian colibacillosis, leading to significant economic losses and concerns for food safety in the poultry industry. This study focused on examining the virulence gene profile, antibiotic resistance prevalence, and resistance patterns of APEC isolates. A total of 250 bacterial strains were collected from birds affected by colibacillosis. Serogrouping revealed diverse serotypes, with O2 being the most common (16%), followed by O1, O8, and O76. All isolates tested positive for at minimum one virulence gene, with 7.2% carrying all five targeted genes, particularly in serogroups O1, O8, O45, and O88. The most detected gene was <i>iss</i>, present in 79.6% of isolates, followed by <i>tsh</i>, <i>iuc</i>C, <i>sitA</i>, and <i>pap</i>C. The antibiotic resistance analysis showed that all isolates exhibited multidrug resistance, although they remained susceptible to gentamicin, amikacin, ciprofloxacin, and chloramphenicol. Moreover, specific antibiotic resistance genes were known in the isolates, with <i>tetA</i> detected in 54.8%, <i>tetB</i> in 51.7%, <i>sul1</i> in 50%, and <i>aadA1</i> in 29.2%. These findings highlight the widespread antibiotic resistance in chicken carcasses, which poses a hazard to human health in terms of transfer of resistance to humans, reduced effectiveness of antibiotics and impaired ability to contain infectious diseases. Therefore, it is crucial to implement strict monitoring programs to regulate antibiotic usage in poultry production.https://www.mdpi.com/2306-7381/12/6/592avian pathogenic <i>Escherichia coli</i>serogroupsvirulencegenesantibiotic resistance |
| spellingShingle | Showkat A. Shah Masood S. Mir Shayaib A. Kamil Majid Shafi Mudasir A. Rather Azmat A. Khan Zahoor A. Wani Sheikh Adil Fatmah M. Alqahtani Majid Alhomrani Manzoor Wani Serogroup Prevalence, Virulence Profile and Antibiotic Resistance of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Chicken Veterinary Sciences avian pathogenic <i>Escherichia coli</i> serogroups virulence genes antibiotic resistance |
| title | Serogroup Prevalence, Virulence Profile and Antibiotic Resistance of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Chicken |
| title_full | Serogroup Prevalence, Virulence Profile and Antibiotic Resistance of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Chicken |
| title_fullStr | Serogroup Prevalence, Virulence Profile and Antibiotic Resistance of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Chicken |
| title_full_unstemmed | Serogroup Prevalence, Virulence Profile and Antibiotic Resistance of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Chicken |
| title_short | Serogroup Prevalence, Virulence Profile and Antibiotic Resistance of Avian Pathogenic <i>Escherichia coli</i> Isolated from Broiler Chicken |
| title_sort | serogroup prevalence virulence profile and antibiotic resistance of avian pathogenic i escherichia coli i isolated from broiler chicken |
| topic | avian pathogenic <i>Escherichia coli</i> serogroups virulence genes antibiotic resistance |
| url | https://www.mdpi.com/2306-7381/12/6/592 |
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