LIF Upregulates Expression of NK-1R in NHBE Cells
Leukemia inhibitory factor (LIF), a cytokine at the interface between neurobiology and immunology, is mainly mediated through JAK/STAT pathway and MAPK/ERK pathway. Evidence suggested LIF is related to the higher expression of neurokinin-1 receptor (NK-1R) in asthma. In this study, the immunohistoch...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/84829 |
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| author | Cheng-Ping Hu Jun-Tao Feng Yu-Ling Tang Jin-Qi Zhu Min-Juan Lin Ming-En Yu |
| author_facet | Cheng-Ping Hu Jun-Tao Feng Yu-Ling Tang Jin-Qi Zhu Min-Juan Lin Ming-En Yu |
| author_sort | Cheng-Ping Hu |
| collection | DOAJ |
| description | Leukemia inhibitory factor (LIF), a cytokine at the interface between neurobiology and immunology, is mainly mediated through JAK/STAT pathway and MAPK/ERK pathway. Evidence suggested LIF is related to the higher expression of neurokinin-1 receptor (NK-1R) in asthma. In this study, the immunohistochemistry stain showed the expressions of NK-1R, LIF, p-STAT3, and p-ERK1/2 in the lung tissues of allergic rats were increased compared with the controls, and the main positive cell type was airway epithelial cell. Normal human bronchial epithelial cells were treated with LIF in the presence or absence of AG490 (JAK2 inhibitor), PD98059 (MEK inhibitor), and the siRNA against STAT3. Western blot and RT-PCR indicated that LIF induced the expression of NK-1R, which was inhibited by the inhibitors mentioned above. No significant interaction was found between JAK/STAT pathway and MAPK/ERK pathway. In summary, bronchial epithelial cell changes in asthma are induced by LIF which promotes the expression of NK-1R, and JAK/STAT pathway and MAPK/ERK pathway may participate in this process. |
| format | Article |
| id | doaj-art-998cc64744334dd5b09b62774d4fa752 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-998cc64744334dd5b09b62774d4fa7522025-02-03T01:23:20ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/8482984829LIF Upregulates Expression of NK-1R in NHBE CellsCheng-Ping Hu0Jun-Tao Feng1Yu-Ling Tang2Jin-Qi Zhu3Min-Juan Lin4Ming-En Yu5Department of Respiratory Medicine, Xiangya Hospital of Central South University, Hunan Province, Changsha 410008, ChinaDepartment of Respiratory Medicine, Xiangya Hospital of Central South University, Hunan Province, Changsha 410008, ChinaDepartment of Respiratory Medicine, Xiangya Hospital of Central South University, Hunan Province, Changsha 410008, ChinaDepartment of Respiratory Medicine, Changsha Central Hospital, Hunan Province, Changsha 410008, ChinaDepartment of Respiratory Medicine, Xiangya Hospital of Central South University, Hunan Province, Changsha 410008, ChinaThird Institute of Oceanography, SOA, Fujian Province, Xiamen 361005, ChinaLeukemia inhibitory factor (LIF), a cytokine at the interface between neurobiology and immunology, is mainly mediated through JAK/STAT pathway and MAPK/ERK pathway. Evidence suggested LIF is related to the higher expression of neurokinin-1 receptor (NK-1R) in asthma. In this study, the immunohistochemistry stain showed the expressions of NK-1R, LIF, p-STAT3, and p-ERK1/2 in the lung tissues of allergic rats were increased compared with the controls, and the main positive cell type was airway epithelial cell. Normal human bronchial epithelial cells were treated with LIF in the presence or absence of AG490 (JAK2 inhibitor), PD98059 (MEK inhibitor), and the siRNA against STAT3. Western blot and RT-PCR indicated that LIF induced the expression of NK-1R, which was inhibited by the inhibitors mentioned above. No significant interaction was found between JAK/STAT pathway and MAPK/ERK pathway. In summary, bronchial epithelial cell changes in asthma are induced by LIF which promotes the expression of NK-1R, and JAK/STAT pathway and MAPK/ERK pathway may participate in this process.http://dx.doi.org/10.1155/MI/2006/84829 |
| spellingShingle | Cheng-Ping Hu Jun-Tao Feng Yu-Ling Tang Jin-Qi Zhu Min-Juan Lin Ming-En Yu LIF Upregulates Expression of NK-1R in NHBE Cells Mediators of Inflammation |
| title | LIF Upregulates Expression of NK-1R in NHBE Cells |
| title_full | LIF Upregulates Expression of NK-1R in NHBE Cells |
| title_fullStr | LIF Upregulates Expression of NK-1R in NHBE Cells |
| title_full_unstemmed | LIF Upregulates Expression of NK-1R in NHBE Cells |
| title_short | LIF Upregulates Expression of NK-1R in NHBE Cells |
| title_sort | lif upregulates expression of nk 1r in nhbe cells |
| url | http://dx.doi.org/10.1155/MI/2006/84829 |
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