Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling
Long-term heavy alcohol consumption could result in a range of health, social, and behavioral problems. People who abuse alcohol are at high risks of seriously having osteopenia, periodontal disease, and compromised oral health. However, the role of ethanol (EtOH) in the biological functions of huma...
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Wiley
2017-01-01
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Series: | Stem Cells International |
Online Access: | http://dx.doi.org/10.1155/2017/8717454 |
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author | Wei Qin Qi-Ting Huang Michael D. Weir Zhi Song Ashraf F. Fouad Zheng-Mei Lin Liang Zhao Hockin H. K. Xu |
author_facet | Wei Qin Qi-Ting Huang Michael D. Weir Zhi Song Ashraf F. Fouad Zheng-Mei Lin Liang Zhao Hockin H. K. Xu |
author_sort | Wei Qin |
collection | DOAJ |
description | Long-term heavy alcohol consumption could result in a range of health, social, and behavioral problems. People who abuse alcohol are at high risks of seriously having osteopenia, periodontal disease, and compromised oral health. However, the role of ethanol (EtOH) in the biological functions of human dental pulp cells (DPCs) is unknown. Whether EtOH affects the odontoblastic differentiation of DPCs through the mechanistic target of rapamycin (mTOR) remains unexplored. The objective of this study was to investigate the effects of EtOH on DPC differentiation and mineralization. DPCs were isolated and purified from human dental pulps. The proliferation and odontoblastic differentiation of DPCs treated with EtOH were subsequently investigated. Different doses of EtOH were shown to be cytocompatible with DPCs. EtOH significantly activated the mTOR pathway in a dose-dependent manner. In addition, EtOH downregulated the alkaline phosphatase activity, attenuated the mineralized nodule formation, and suppressed the expression of odontoblastic markers including ALP, DSPP, DMP-1, Runx2, and OCN. Moreover, the pretreatment with rapamycin, a specific mTOR inhibitor, markedly reversed the EtOH-induced odontoblastic differentiation and cell mineralization. Our findings show for the first time that EtOH can suppress DPC differentiation and mineralization in a mTOR-dependent manner, indicating that EtOH may be involved in negatively regulating the dental pulp repair. |
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institution | Kabale University |
issn | 1687-966X 1687-9678 |
language | English |
publishDate | 2017-01-01 |
publisher | Wiley |
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spelling | doaj-art-998af08dfc8d4ab08691a9f7e066af722025-02-03T01:32:29ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/87174548717454Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR SignalingWei Qin0Qi-Ting Huang1Michael D. Weir2Zhi Song3Ashraf F. Fouad4Zheng-Mei Lin5Liang Zhao6Hockin H. K. Xu7Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, ChinaDepartment of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, ChinaBiomaterials and Tissue Engineering Division, Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USADepartment of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, ChinaDepartment of Endodontics, School of Dentistry, University of North Carolina, Chapel Hill, NC 27599-7450, USADepartment of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, ChinaBiomaterials and Tissue Engineering Division, Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USABiomaterials and Tissue Engineering Division, Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USALong-term heavy alcohol consumption could result in a range of health, social, and behavioral problems. People who abuse alcohol are at high risks of seriously having osteopenia, periodontal disease, and compromised oral health. However, the role of ethanol (EtOH) in the biological functions of human dental pulp cells (DPCs) is unknown. Whether EtOH affects the odontoblastic differentiation of DPCs through the mechanistic target of rapamycin (mTOR) remains unexplored. The objective of this study was to investigate the effects of EtOH on DPC differentiation and mineralization. DPCs were isolated and purified from human dental pulps. The proliferation and odontoblastic differentiation of DPCs treated with EtOH were subsequently investigated. Different doses of EtOH were shown to be cytocompatible with DPCs. EtOH significantly activated the mTOR pathway in a dose-dependent manner. In addition, EtOH downregulated the alkaline phosphatase activity, attenuated the mineralized nodule formation, and suppressed the expression of odontoblastic markers including ALP, DSPP, DMP-1, Runx2, and OCN. Moreover, the pretreatment with rapamycin, a specific mTOR inhibitor, markedly reversed the EtOH-induced odontoblastic differentiation and cell mineralization. Our findings show for the first time that EtOH can suppress DPC differentiation and mineralization in a mTOR-dependent manner, indicating that EtOH may be involved in negatively regulating the dental pulp repair.http://dx.doi.org/10.1155/2017/8717454 |
spellingShingle | Wei Qin Qi-Ting Huang Michael D. Weir Zhi Song Ashraf F. Fouad Zheng-Mei Lin Liang Zhao Hockin H. K. Xu Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling Stem Cells International |
title | Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling |
title_full | Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling |
title_fullStr | Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling |
title_full_unstemmed | Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling |
title_short | Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling |
title_sort | alcohol inhibits odontogenic differentiation of human dental pulp cells by activating mtor signaling |
url | http://dx.doi.org/10.1155/2017/8717454 |
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