<i>Caulobacter</i> Strains Code for Novel Restriction Endonucleases That Protect Them from Bacteriophage Infections
Bacteriophages grown on <i>Caulobacter vibrioides</i> strain CB15 have reduced plating efficiency on other <i>Caulobacter</i> strains. To determine the cause of this reduced plating efficiency, we performed a series of experiments that demonstrated that the reduced plating ef...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/17/3/311 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850087645386899456 |
|---|---|
| author | Ian Sisto Bert Ely |
| author_facet | Ian Sisto Bert Ely |
| author_sort | Ian Sisto |
| collection | DOAJ |
| description | Bacteriophages grown on <i>Caulobacter vibrioides</i> strain CB15 have reduced plating efficiency on other <i>Caulobacter</i> strains. To determine the cause of this reduced plating efficiency, we performed a series of experiments that demonstrated that the reduced plating efficiency is due to a novel set of restriction and modification (RM) enzymes that are present in most of the <i>Caulobacter</i> strains that we tested. We then demonstrated that one of these RM systems recognizes the nucleotide sequence 5′-ATNNAT-3′. A careful inspection of the genome nucleotide sequences of each of the strains revealed that the genes coding for these RM enzymes have not been annotated or identified, suggesting that the proteins may differ from the common types of bacterial restriction and modification enzymes. In addition, the host strain NA1000 contains a 26 kb mobile element that provides resistance to incoming phages. |
| format | Article |
| id | doaj-art-9976134c490f4d4cb7e7597246169ece |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-9976134c490f4d4cb7e7597246169ece2025-08-20T02:43:10ZengMDPI AGViruses1999-49152025-02-0117331110.3390/v17030311<i>Caulobacter</i> Strains Code for Novel Restriction Endonucleases That Protect Them from Bacteriophage InfectionsIan Sisto0Bert Ely1Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USADepartment of Biological Sciences, University of South Carolina, Columbia, SC 29208, USABacteriophages grown on <i>Caulobacter vibrioides</i> strain CB15 have reduced plating efficiency on other <i>Caulobacter</i> strains. To determine the cause of this reduced plating efficiency, we performed a series of experiments that demonstrated that the reduced plating efficiency is due to a novel set of restriction and modification (RM) enzymes that are present in most of the <i>Caulobacter</i> strains that we tested. We then demonstrated that one of these RM systems recognizes the nucleotide sequence 5′-ATNNAT-3′. A careful inspection of the genome nucleotide sequences of each of the strains revealed that the genes coding for these RM enzymes have not been annotated or identified, suggesting that the proteins may differ from the common types of bacterial restriction and modification enzymes. In addition, the host strain NA1000 contains a 26 kb mobile element that provides resistance to incoming phages.https://www.mdpi.com/1999-4915/17/3/311<i>Caulobacter</i>restriction enzymesphage plating efficiencybacteriophageDNA methylation |
| spellingShingle | Ian Sisto Bert Ely <i>Caulobacter</i> Strains Code for Novel Restriction Endonucleases That Protect Them from Bacteriophage Infections Viruses <i>Caulobacter</i> restriction enzymes phage plating efficiency bacteriophage DNA methylation |
| title | <i>Caulobacter</i> Strains Code for Novel Restriction Endonucleases That Protect Them from Bacteriophage Infections |
| title_full | <i>Caulobacter</i> Strains Code for Novel Restriction Endonucleases That Protect Them from Bacteriophage Infections |
| title_fullStr | <i>Caulobacter</i> Strains Code for Novel Restriction Endonucleases That Protect Them from Bacteriophage Infections |
| title_full_unstemmed | <i>Caulobacter</i> Strains Code for Novel Restriction Endonucleases That Protect Them from Bacteriophage Infections |
| title_short | <i>Caulobacter</i> Strains Code for Novel Restriction Endonucleases That Protect Them from Bacteriophage Infections |
| title_sort | i caulobacter i strains code for novel restriction endonucleases that protect them from bacteriophage infections |
| topic | <i>Caulobacter</i> restriction enzymes phage plating efficiency bacteriophage DNA methylation |
| url | https://www.mdpi.com/1999-4915/17/3/311 |
| work_keys_str_mv | AT iansisto icaulobacteristrainscodefornovelrestrictionendonucleasesthatprotectthemfrombacteriophageinfections AT bertely icaulobacteristrainscodefornovelrestrictionendonucleasesthatprotectthemfrombacteriophageinfections |