Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic material
Background. High-molecular-weight glutenin subunits (HMW-GSs) make the greatest contribution to the formation of baking properties in bread wheat (Triticum aestivum L.). Glutenin polymorphism of bread wheat relatives is significantly richer than in cultivated varieties. The objective of this work wa...
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N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2024-05-01
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| Series: | Труды по прикладной ботанике, генетике и селекции |
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| Online Access: | https://elpub.vir.nw.ru/jour/article/view/1874 |
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| author | O. A. Orlovskaya S. I. Vakula L. V. Khotyleva A. V. Kilchevsky |
| author_facet | O. A. Orlovskaya S. I. Vakula L. V. Khotyleva A. V. Kilchevsky |
| author_sort | O. A. Orlovskaya |
| collection | DOAJ |
| description | Background. High-molecular-weight glutenin subunits (HMW-GSs) make the greatest contribution to the formation of baking properties in bread wheat (Triticum aestivum L.). Glutenin polymorphism of bread wheat relatives is significantly richer than in cultivated varieties. The objective of this work was to identify the HMW-GS composition and assess the gluten quality of bread wheat lines with introgressions of alien genetic material.Materials and methods. We studied the parental varieties of spring bread wheat, accessions of the tetraploid and hexaploid Triticum L. species, and 19 introgressive lines produced with their participation. Glutenins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis and HMW-GSs were identified using the Payne nomenclature system. Gluten quality was determined in accordance with GOST 13586.1-68 (the 2017–2019 and 2021–2022 growing seasons). Data processing was performed using the Statistica 10.0 and MS Excel software packages.Results and conclusion. HMW-GSs uncharacteristic of T. aestivum cultivars were identified in the studied accessions of T. dicoccoides (Körn. ex Aschers. et Graebn.) Schweinf., T. spelta L., and T. kiharae Dorof. et Migusch. These HMW-GSs are of interest for enriching the wheat gene pool. HMW-GSs of related species were found in 10 out of 19 introgressive lines. A five-year observation period revealed that wheat relatives on average significantly exceeded common wheat cultivars in gluten quality, and introgressive lines did not differ significantly from the parental genotypes. Lines with high rheological properties of gluten had, as a rule, HMW-GSs of related species in their composition. |
| format | Article |
| id | doaj-art-9974aeda90a44234bfa3a6928df1e71e |
| institution | Kabale University |
| issn | 2227-8834 2619-0982 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Труды по прикладной ботанике, генетике и селекции |
| spelling | doaj-art-9974aeda90a44234bfa3a6928df1e71e2025-02-03T08:31:16ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822024-05-01185115216010.30901/2227-8834-2024-1-152-160780Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic materialO. A. Orlovskaya0S. I. Vakula1L. V. Khotyleva2A. V. Kilchevsky3Institute of Genetics and Cytology of the National Academy of Sciences of BelarusBelarusian State Technological UniversityInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusBackground. High-molecular-weight glutenin subunits (HMW-GSs) make the greatest contribution to the formation of baking properties in bread wheat (Triticum aestivum L.). Glutenin polymorphism of bread wheat relatives is significantly richer than in cultivated varieties. The objective of this work was to identify the HMW-GS composition and assess the gluten quality of bread wheat lines with introgressions of alien genetic material.Materials and methods. We studied the parental varieties of spring bread wheat, accessions of the tetraploid and hexaploid Triticum L. species, and 19 introgressive lines produced with their participation. Glutenins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis and HMW-GSs were identified using the Payne nomenclature system. Gluten quality was determined in accordance with GOST 13586.1-68 (the 2017–2019 and 2021–2022 growing seasons). Data processing was performed using the Statistica 10.0 and MS Excel software packages.Results and conclusion. HMW-GSs uncharacteristic of T. aestivum cultivars were identified in the studied accessions of T. dicoccoides (Körn. ex Aschers. et Graebn.) Schweinf., T. spelta L., and T. kiharae Dorof. et Migusch. These HMW-GSs are of interest for enriching the wheat gene pool. HMW-GSs of related species were found in 10 out of 19 introgressive lines. A five-year observation period revealed that wheat relatives on average significantly exceeded common wheat cultivars in gluten quality, and introgressive lines did not differ significantly from the parental genotypes. Lines with high rheological properties of gluten had, as a rule, HMW-GSs of related species in their composition.https://elpub.vir.nw.ru/jour/article/view/1874triticum aestivum l.wheat relativesintrogressive bread wheat linessds–pageglu-1 locigrain quality |
| spellingShingle | O. A. Orlovskaya S. I. Vakula L. V. Khotyleva A. V. Kilchevsky Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic material Труды по прикладной ботанике, генетике и селекции triticum aestivum l. wheat relatives introgressive bread wheat lines sds–page glu-1 loci grain quality |
| title | Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic material |
| title_full | Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic material |
| title_fullStr | Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic material |
| title_full_unstemmed | Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic material |
| title_short | Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic material |
| title_sort | composition of high molecular weight glutenin subunits and gluten quality in wheat lines with alien genetic material |
| topic | triticum aestivum l. wheat relatives introgressive bread wheat lines sds–page glu-1 loci grain quality |
| url | https://elpub.vir.nw.ru/jour/article/view/1874 |
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