Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production.
A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089887&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849720281914933248 |
|---|---|
| author | Yan Li Gary W Lawrence Shien Lu Clarissa Balbalian Vincent P Klink |
| author_facet | Yan Li Gary W Lawrence Shien Lu Clarissa Balbalian Vincent P Klink |
| author_sort | Yan Li |
| collection | DOAJ |
| description | A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil. |
| format | Article |
| id | doaj-art-9967ec27132d468984e30d1349a32e76 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-9967ec27132d468984e30d1349a32e762025-08-20T03:11:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8988710.1371/journal.pone.0089887Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production.Yan LiGary W LawrenceShien LuClarissa BalbalianVincent P KlinkA quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089887&type=printable |
| spellingShingle | Yan Li Gary W Lawrence Shien Lu Clarissa Balbalian Vincent P Klink Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production. PLoS ONE |
| title | Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production. |
| title_full | Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production. |
| title_fullStr | Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production. |
| title_full_unstemmed | Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production. |
| title_short | Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production. |
| title_sort | quantitative field testing heterodera glycines from metagenomic dna samples isolated directly from soil under agronomic production |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089887&type=printable |
| work_keys_str_mv | AT yanli quantitativefieldtestingheteroderaglycinesfrommetagenomicdnasamplesisolateddirectlyfromsoilunderagronomicproduction AT garywlawrence quantitativefieldtestingheteroderaglycinesfrommetagenomicdnasamplesisolateddirectlyfromsoilunderagronomicproduction AT shienlu quantitativefieldtestingheteroderaglycinesfrommetagenomicdnasamplesisolateddirectlyfromsoilunderagronomicproduction AT clarissabalbalian quantitativefieldtestingheteroderaglycinesfrommetagenomicdnasamplesisolateddirectlyfromsoilunderagronomicproduction AT vincentpklink quantitativefieldtestingheteroderaglycinesfrommetagenomicdnasamplesisolateddirectlyfromsoilunderagronomicproduction |