Astragaloside IV represses hepatocellular carcinoma progression by modulating HMGB1-ferroptosis axis
Abstract Astragaloside IV (AST-IV), as one of the main functional components of Astragalus membranaceus, has physiological functions such as regulating metabolism and anti-tumor. However, the role of AST-IV on hepatocellular carcinoma (HCC) was still poorly understood. In this study, our work explor...
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| Format: | Article |
| Language: | English |
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Springer
2025-08-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-03286-5 |
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| author | Xingyang Zhao Ruizhe Liu Haiyu Zhou Deqing Wu |
| author_facet | Xingyang Zhao Ruizhe Liu Haiyu Zhou Deqing Wu |
| author_sort | Xingyang Zhao |
| collection | DOAJ |
| description | Abstract Astragaloside IV (AST-IV), as one of the main functional components of Astragalus membranaceus, has physiological functions such as regulating metabolism and anti-tumor. However, the role of AST-IV on hepatocellular carcinoma (HCC) was still poorly understood. In this study, our work explored whether AST-IV could induce ferroptosis and repress HCC tumorigenesis. Results indicated that AST-IV could repress the tumor progression (viability, migration) of HCC in vitro. Besides, AST-IV induced the ferroptosis (Fe2+, malondialdehyde, lipid peroxidation) in HCC cells. Molecular docking and microscale thermophoresis indicated that high mobility group protein B1 (HMGB1) acted as the target of AST-IV. AST-IV could repress the HMGB1 expression and HMGB1 reversed the role of AST-IV on HCC cells’ ferroptosis. In vivo, AST-IV administration repressed the tumor progression. In conclusion, AST-IV represses HCC progression by modulating HMGB1-ferroptosis axis, which provides a novel insight for HCC. |
| format | Article |
| id | doaj-art-9957a2a1925b4af3ba97174ae211aa01 |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-9957a2a1925b4af3ba97174ae211aa012025-08-20T03:46:17ZengSpringerDiscover Oncology2730-60112025-08-0116111110.1007/s12672-025-03286-5Astragaloside IV represses hepatocellular carcinoma progression by modulating HMGB1-ferroptosis axisXingyang Zhao0Ruizhe Liu1Haiyu Zhou2Deqing Wu3Department of General Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital Ganzhou Hospital, Guangdong Academy of Medical SciencesDepartment of Thoracic Surgery, Guangdong Provincial People’s Hospital, Guangdong Cardiovascular Institute, Guangdong Academy of Medical SciencesDepartment of Thoracic Surgery, Guangdong Provincial People’s Hospital, Guangdong Cardiovascular Institute, Guangdong Academy of Medical SciencesDepartment of General Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital Ganzhou Hospital, Guangdong Academy of Medical SciencesAbstract Astragaloside IV (AST-IV), as one of the main functional components of Astragalus membranaceus, has physiological functions such as regulating metabolism and anti-tumor. However, the role of AST-IV on hepatocellular carcinoma (HCC) was still poorly understood. In this study, our work explored whether AST-IV could induce ferroptosis and repress HCC tumorigenesis. Results indicated that AST-IV could repress the tumor progression (viability, migration) of HCC in vitro. Besides, AST-IV induced the ferroptosis (Fe2+, malondialdehyde, lipid peroxidation) in HCC cells. Molecular docking and microscale thermophoresis indicated that high mobility group protein B1 (HMGB1) acted as the target of AST-IV. AST-IV could repress the HMGB1 expression and HMGB1 reversed the role of AST-IV on HCC cells’ ferroptosis. In vivo, AST-IV administration repressed the tumor progression. In conclusion, AST-IV represses HCC progression by modulating HMGB1-ferroptosis axis, which provides a novel insight for HCC.https://doi.org/10.1007/s12672-025-03286-5Astragaloside IVHepatocellular carcinomaHMGB1Ferroptosis |
| spellingShingle | Xingyang Zhao Ruizhe Liu Haiyu Zhou Deqing Wu Astragaloside IV represses hepatocellular carcinoma progression by modulating HMGB1-ferroptosis axis Discover Oncology Astragaloside IV Hepatocellular carcinoma HMGB1 Ferroptosis |
| title | Astragaloside IV represses hepatocellular carcinoma progression by modulating HMGB1-ferroptosis axis |
| title_full | Astragaloside IV represses hepatocellular carcinoma progression by modulating HMGB1-ferroptosis axis |
| title_fullStr | Astragaloside IV represses hepatocellular carcinoma progression by modulating HMGB1-ferroptosis axis |
| title_full_unstemmed | Astragaloside IV represses hepatocellular carcinoma progression by modulating HMGB1-ferroptosis axis |
| title_short | Astragaloside IV represses hepatocellular carcinoma progression by modulating HMGB1-ferroptosis axis |
| title_sort | astragaloside iv represses hepatocellular carcinoma progression by modulating hmgb1 ferroptosis axis |
| topic | Astragaloside IV Hepatocellular carcinoma HMGB1 Ferroptosis |
| url | https://doi.org/10.1007/s12672-025-03286-5 |
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