Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based as...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2014/535789 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551617670414336 |
|---|---|
| author | Tatiane Oliveira Camila A. Figueiredo Carlos Brito Alexander Stavroullakis Anuradha Prakki Eudes Da Silva Velozo Getulio Nogueira-Filho |
| author_facet | Tatiane Oliveira Camila A. Figueiredo Carlos Brito Alexander Stavroullakis Anuradha Prakki Eudes Da Silva Velozo Getulio Nogueira-Filho |
| author_sort | Tatiane Oliveira |
| collection | DOAJ |
| description | Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. Osteoclast precursor cells (RAW 264.7) were stimulated by Pg LPS (1 μg/mL) and E. coli LPS (1 μg/mL) in the presence or absence of different concentrations of AcE (10–1000 μg/mL) for 5 days at 37°C/5% CO2. Resazurin reduction and total protein content assays were used to detect cell viability. AcE did not affect cell viability. Resazurin reduction assay showed that AcE, at up to 1000 μg/mL, did not significantly affect cell viability and cellular protein levels. Additionally a caspase 3/7 luminescence assay was used to disclose apoptosis and there was no difference in apoptotic activity between tested groups and control group. Fluorescence images stained by DAPI showed no alteration on the morphology and cell counts of LPS-stimulated osteoclast precursor cells with the use of AcE in all tested concentrations when compared to control. These findings suggest that Allium cepa L. extract could be used for in vitro studies on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells. |
| format | Article |
| id | doaj-art-9942868733a349528c96a18a0ced94ff |
| institution | Kabale University |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-9942868733a349528c96a18a0ced94ff2025-02-03T06:01:02ZengWileyInternational Journal of Cell Biology1687-88761687-88842014-01-01201410.1155/2014/535789535789Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-StainingTatiane Oliveira0Camila A. Figueiredo1Carlos Brito2Alexander Stavroullakis3Anuradha Prakki4Eudes Da Silva Velozo5Getulio Nogueira-Filho6Department of Bioregulation, Institute of Health Sciences, Federal University of Bahia, 41110-100 Salvador, BA, BrazilDepartment of Bioregulation, Institute of Health Sciences, Federal University of Bahia, 41110-100 Salvador, BA, BrazilDepartment of Biological and Diagnostic Sciences-Preventive Dentistry, University of Toronto, Toronto, ON, M5G 1G6, CanadaDepartment of Biological and Diagnostic Sciences-Preventive Dentistry, University of Toronto, Toronto, ON, M5G 1G6, CanadaDepartment of Clinical Sciences-Restorative, University of Toronto, Toronto, ON, M5G 1G6, CanadaDepartment of Medicines, Faculty of Pharmacy, Federal University of Bahia, 40170-115 Salvador, BA, BrazilDepartment of Biological and Diagnostic Sciences-Preventive Dentistry, University of Toronto, Toronto, ON, M5G 1G6, CanadaAllium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. Osteoclast precursor cells (RAW 264.7) were stimulated by Pg LPS (1 μg/mL) and E. coli LPS (1 μg/mL) in the presence or absence of different concentrations of AcE (10–1000 μg/mL) for 5 days at 37°C/5% CO2. Resazurin reduction and total protein content assays were used to detect cell viability. AcE did not affect cell viability. Resazurin reduction assay showed that AcE, at up to 1000 μg/mL, did not significantly affect cell viability and cellular protein levels. Additionally a caspase 3/7 luminescence assay was used to disclose apoptosis and there was no difference in apoptotic activity between tested groups and control group. Fluorescence images stained by DAPI showed no alteration on the morphology and cell counts of LPS-stimulated osteoclast precursor cells with the use of AcE in all tested concentrations when compared to control. These findings suggest that Allium cepa L. extract could be used for in vitro studies on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells.http://dx.doi.org/10.1155/2014/535789 |
| spellingShingle | Tatiane Oliveira Camila A. Figueiredo Carlos Brito Alexander Stavroullakis Anuradha Prakki Eudes Da Silva Velozo Getulio Nogueira-Filho Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining International Journal of Cell Biology |
| title | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
| title_full | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
| title_fullStr | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
| title_full_unstemmed | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
| title_short | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
| title_sort | effect of allium cepa l on lipopolysaccharide stimulated osteoclast precursor cell viability count and morphology using 4 6 diamidino 2 phenylindole staining |
| url | http://dx.doi.org/10.1155/2014/535789 |
| work_keys_str_mv | AT tatianeoliveira effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT camilaafigueiredo effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT carlosbrito effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT alexanderstavroullakis effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT anuradhaprakki effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT eudesdasilvavelozo effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT getulionogueirafilho effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining |