Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopy
Summary: Here, we present a protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-computed tomography (CT) and ultraviolet-visible (UV-vis) spectroscopy. We describe steps for sample fixation, a staining procedure with iodine-based contras...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003570 |
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| _version_ | 1849319733150613504 |
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| author | Federica Orellana Annapaola Parrilli |
| author_facet | Federica Orellana Annapaola Parrilli |
| author_sort | Federica Orellana |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-computed tomography (CT) and ultraviolet-visible (UV-vis) spectroscopy. We describe steps for sample fixation, a staining procedure with iodine-based contrast agents, and imaging using micro-CT. We then detail procedures for volumetric and radiodensity analyses, UV-vis spectroscopy, and data processing.For complete details on the use and execution of this protocol, please refer to Orellana et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-993bd6b38d174cc49324181e9409e42b |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-993bd6b38d174cc49324181e9409e42b2025-08-20T03:50:21ZengElsevierSTAR Protocols2666-16672025-09-016310395110.1016/j.xpro.2025.103951Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopyFederica Orellana0Annapaola Parrilli1Empa - Swiss Federal Laboratories for Materials Science and Technology, 8600 Dübendorf, Switzerland; Corresponding authorEmpa - Swiss Federal Laboratories for Materials Science and Technology, 8600 Dübendorf, Switzerland; Corresponding authorSummary: Here, we present a protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-computed tomography (CT) and ultraviolet-visible (UV-vis) spectroscopy. We describe steps for sample fixation, a staining procedure with iodine-based contrast agents, and imaging using micro-CT. We then detail procedures for volumetric and radiodensity analyses, UV-vis spectroscopy, and data processing.For complete details on the use and execution of this protocol, please refer to Orellana et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725003570Health SciencesMicroscopyModel Organisms |
| spellingShingle | Federica Orellana Annapaola Parrilli Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopy STAR Protocols Health Sciences Microscopy Model Organisms |
| title | Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopy |
| title_full | Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopy |
| title_fullStr | Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopy |
| title_full_unstemmed | Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopy |
| title_short | Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopy |
| title_sort | protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast enhanced micro ct and uv vis spectroscopy |
| topic | Health Sciences Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725003570 |
| work_keys_str_mv | AT federicaorellana protocolforevaluatingiodineuptakeanddiffusioninsheepandpigmeniscustissueusingcontrastenhancedmicroctanduvvisspectroscopy AT annapaolaparrilli protocolforevaluatingiodineuptakeanddiffusioninsheepandpigmeniscustissueusingcontrastenhancedmicroctanduvvisspectroscopy |