Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopy
Summary: Here, we present a protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-computed tomography (CT) and ultraviolet-visible (UV-vis) spectroscopy. We describe steps for sample fixation, a staining procedure with iodine-based contras...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003570 |
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| Summary: | Summary: Here, we present a protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-computed tomography (CT) and ultraviolet-visible (UV-vis) spectroscopy. We describe steps for sample fixation, a staining procedure with iodine-based contrast agents, and imaging using micro-CT. We then detail procedures for volumetric and radiodensity analyses, UV-vis spectroscopy, and data processing.For complete details on the use and execution of this protocol, please refer to Orellana et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |