Guava Leaf Extract Exhibits Antimicrobial Activity in Extensively Drug-Resistant (XDR) <i>Acinetobacter baumannii</i>
Currently, a global health crisis is being caused by microbial resistance, in which <i>Acinetobacter baumannii</i> plays a crucial role, being considered the highest-priority microorganism by the World Health Organization (WHO) for discovering new antibiotics. As a result, phytochemicals...
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2024-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/1/70 |
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| author | Daniela Gutierrez-Montiel Alma L. Guerrero-Barrera Flor Y. Ramírez-Castillo Fabiola Galindo-Guerrero Ingrid G. Ornelas-García Norma A. Chávez-Vela Matheus de O. Costa Francisco J. Avelar-Gonzalez Adriana C. Moreno-Flores Erick Vazquez-Pedroza José M. Arreola-Guerra Mario González-Gámez |
| author_facet | Daniela Gutierrez-Montiel Alma L. Guerrero-Barrera Flor Y. Ramírez-Castillo Fabiola Galindo-Guerrero Ingrid G. Ornelas-García Norma A. Chávez-Vela Matheus de O. Costa Francisco J. Avelar-Gonzalez Adriana C. Moreno-Flores Erick Vazquez-Pedroza José M. Arreola-Guerra Mario González-Gámez |
| author_sort | Daniela Gutierrez-Montiel |
| collection | DOAJ |
| description | Currently, a global health crisis is being caused by microbial resistance, in which <i>Acinetobacter baumannii</i> plays a crucial role, being considered the highest-priority microorganism by the World Health Organization (WHO) for discovering new antibiotics. As a result, phytochemicals have emerged as a potential alternative to combat resistant strains, since they can exert antimicrobial activity through various mechanisms and, at the same time, represent a more natural and safe option. This study analyzes the antimicrobial effects of guava leaf extract in ten clinical isolates of extensively drug-resistant (XDR) <i>A. baumannii</i>, using the agar diffusion technique and the microdilution method to determine the minimum inhibitory concentrations (MICs). Additionally, possible improvements in antimicrobial activity after the purification of polyphenolic compounds and potential synergy with the antibiotic gentamicin are examined in this research. Moreover, the effect of the plant extract in cell line A549 derived from lung tissue was also evaluated. The extract exhibited antimicrobial activity against all the strains studied, and the purification of polyphenols along with the combination with gentamicin improved the extract activity. The presence of the plant extract induced morphological changes in the lung cells after 24 h of exposure. Therefore, <i>Psidium guajava</i> L. leaf extract is a potential antimicrobial agent. |
| format | Article |
| id | doaj-art-9939a82a0baf474b954f2fb0566da1bc |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-9939a82a0baf474b954f2fb0566da1bc2025-01-10T13:18:47ZengMDPI AGMolecules1420-30492024-12-013017010.3390/molecules30010070Guava Leaf Extract Exhibits Antimicrobial Activity in Extensively Drug-Resistant (XDR) <i>Acinetobacter baumannii</i>Daniela Gutierrez-Montiel0Alma L. Guerrero-Barrera1Flor Y. Ramírez-Castillo2Fabiola Galindo-Guerrero3Ingrid G. Ornelas-García4Norma A. Chávez-Vela5Matheus de O. Costa6Francisco J. Avelar-Gonzalez7Adriana C. Moreno-Flores8Erick Vazquez-Pedroza9José M. Arreola-Guerra10Mario González-Gámez11Laboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, MexicoLaboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, MexicoLaboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, MexicoLaboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, MexicoLaboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, MexicoLaboratorio de Biotecnología, Departamento Ingeniería Bioquímica, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, MexicoLarge Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, CanadaLaboratorio de Estudios Ambientales, Departamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, MexicoLaboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, MexicoLaboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, MexicoDepartamento de Nefrología, Hospital Centenario Miguel Hidalgo, Aguascalientes 20240, MexicoDepartamento de Nefrología, Hospital Centenario Miguel Hidalgo, Aguascalientes 20240, MexicoCurrently, a global health crisis is being caused by microbial resistance, in which <i>Acinetobacter baumannii</i> plays a crucial role, being considered the highest-priority microorganism by the World Health Organization (WHO) for discovering new antibiotics. As a result, phytochemicals have emerged as a potential alternative to combat resistant strains, since they can exert antimicrobial activity through various mechanisms and, at the same time, represent a more natural and safe option. This study analyzes the antimicrobial effects of guava leaf extract in ten clinical isolates of extensively drug-resistant (XDR) <i>A. baumannii</i>, using the agar diffusion technique and the microdilution method to determine the minimum inhibitory concentrations (MICs). Additionally, possible improvements in antimicrobial activity after the purification of polyphenolic compounds and potential synergy with the antibiotic gentamicin are examined in this research. Moreover, the effect of the plant extract in cell line A549 derived from lung tissue was also evaluated. The extract exhibited antimicrobial activity against all the strains studied, and the purification of polyphenols along with the combination with gentamicin improved the extract activity. The presence of the plant extract induced morphological changes in the lung cells after 24 h of exposure. Therefore, <i>Psidium guajava</i> L. leaf extract is a potential antimicrobial agent.https://www.mdpi.com/1420-3049/30/1/70<i>Psidium guajava</i> L.<i>Acinetobacter baumannii</i>extensively drug-resistantantimicrobial activityguava leaf extractphytochemicals |
| spellingShingle | Daniela Gutierrez-Montiel Alma L. Guerrero-Barrera Flor Y. Ramírez-Castillo Fabiola Galindo-Guerrero Ingrid G. Ornelas-García Norma A. Chávez-Vela Matheus de O. Costa Francisco J. Avelar-Gonzalez Adriana C. Moreno-Flores Erick Vazquez-Pedroza José M. Arreola-Guerra Mario González-Gámez Guava Leaf Extract Exhibits Antimicrobial Activity in Extensively Drug-Resistant (XDR) <i>Acinetobacter baumannii</i> Molecules <i>Psidium guajava</i> L. <i>Acinetobacter baumannii</i> extensively drug-resistant antimicrobial activity guava leaf extract phytochemicals |
| title | Guava Leaf Extract Exhibits Antimicrobial Activity in Extensively Drug-Resistant (XDR) <i>Acinetobacter baumannii</i> |
| title_full | Guava Leaf Extract Exhibits Antimicrobial Activity in Extensively Drug-Resistant (XDR) <i>Acinetobacter baumannii</i> |
| title_fullStr | Guava Leaf Extract Exhibits Antimicrobial Activity in Extensively Drug-Resistant (XDR) <i>Acinetobacter baumannii</i> |
| title_full_unstemmed | Guava Leaf Extract Exhibits Antimicrobial Activity in Extensively Drug-Resistant (XDR) <i>Acinetobacter baumannii</i> |
| title_short | Guava Leaf Extract Exhibits Antimicrobial Activity in Extensively Drug-Resistant (XDR) <i>Acinetobacter baumannii</i> |
| title_sort | guava leaf extract exhibits antimicrobial activity in extensively drug resistant xdr i acinetobacter baumannii i |
| topic | <i>Psidium guajava</i> L. <i>Acinetobacter baumannii</i> extensively drug-resistant antimicrobial activity guava leaf extract phytochemicals |
| url | https://www.mdpi.com/1420-3049/30/1/70 |
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