Vitamin D levels and its influencing factors in children and adolescents in mainland China: a systematic review and meta-analysis

Objectives Childhood vitamin D deficiency is a public health issue. This study aims to systematically evaluate vitamin D nutritional status among children and adolescents in Mainland China through a quantitative analysis of literature, providing evidence-based strategies for prevention.Design This i...

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Main Authors: Bo Chen, Pengyun Ji, Zisheng Li, Wenyan Qin, Youqing She
Format: Article
Language:English
Published: BMJ Publishing Group 2025-06-01
Series:BMJ Open
Online Access:https://bmjopen.bmj.com/content/15/6/e094693.full
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author Bo Chen
Pengyun Ji
Zisheng Li
Wenyan Qin
Youqing She
author_facet Bo Chen
Pengyun Ji
Zisheng Li
Wenyan Qin
Youqing She
author_sort Bo Chen
collection DOAJ
description Objectives Childhood vitamin D deficiency is a public health issue. This study aims to systematically evaluate vitamin D nutritional status among children and adolescents in Mainland China through a quantitative analysis of literature, providing evidence-based strategies for prevention.Design This is a systematic review and meta-analysis, conducted following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.Data sources A comprehensive search strategy was implemented across eight electronic databases (PubMed, Embase, Web of Science, Cochrane Library, CNKI, VIP, Wanfang and CBM) from inception to February 2024.Eligibility criteria We included cross-sectional studies that measured serum 25-hydroxyvitamin D levels and analysed influencing factors (eg, age, season, region) in healthy children and adolescents aged 0–18 years in Mainland China; studies reporting prevalence data of vitamin D deficiency/insufficiency based on standardised thresholds (deficiency <50 nmol/L, insufficiency 50–75 nmol/L) and using validated detection methods (eg, liquid chromatography-tandem mass spectrometry, chemiluminescence immunoassay, ELISA) were prioritised.Data extraction and synthesis Two independent reviewers systematically searched, screened and extracted data using predefined protocols. Study quality was assessed with the Agency for Healthcare Research and Quality (AHRQ) tool. Meta-analyses were performed using random-effects models in Review Manager V.5.3 and Stata V.16.0, with subgroup analyses by age, season and region. Sensitivity analysis and Egger’s test were applied to evaluate robustness and publication bias. Findings were synthesised through narrative summaries and quantitative pooling.Results The pooled prevalence of vitamin D deficiency among children and adolescents in Mainland China was 48% (95% CI: 40% to 57%), with extreme heterogeneity across studies (I² = 99.98%, p<0.001). Sensitivity analysis confirmed the stability of pooled estimates. Subgroup analyses revealed significant age-related declines (infants: 82.35 nmol/L vs adolescents: 50.98 nmol/L, p<0.05). Study quality assessed by AHRQ criteria showed 16% of included studies were high-quality (scores 8-11/11), 84% moderate (4-7). Evidence of publication bias was detected via Egger’s test (p<0.05) and funnel plot asymmetry.Conclusions The study highlights the widespread nature of vitamin D deficiency among children and adolescents in Mainland China, particularly in older children and during winter months. Effective interventions are necessary to address this issue. Future research should prioritise methodological standardisation to reduce heterogeneity and address potential publication bias.PROSPERO registration number CRD42023479183.
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spelling doaj-art-9934dff1714042e48c2a7de3737d71fc2025-08-20T03:24:03ZengBMJ Publishing GroupBMJ Open2044-60552025-06-0115610.1136/bmjopen-2024-094693Vitamin D levels and its influencing factors in children and adolescents in mainland China: a systematic review and meta-analysisBo Chen0Pengyun Ji1Zisheng Li2Wenyan Qin3Youqing She4Department of Nuclear Medicine, Chaohu Hospital of Anhui Medical University, Hefei, Anhui, ChinaDepartment of Pain, Eastern Theater General Hospital, Nanjing, Jiangsu, ChinaDepartment of Nuclear Medicine, Chaohu Hospital of Anhui Medical University, Hefei, Anhui, ChinaDepartment of Nuclear Medicine, Chaohu Hospital of Anhui Medical University, Hefei, Anhui, ChinaDepartment of Nuclear Medicine, Chaohu Hospital of Anhui Medical University, Hefei, Anhui, ChinaObjectives Childhood vitamin D deficiency is a public health issue. This study aims to systematically evaluate vitamin D nutritional status among children and adolescents in Mainland China through a quantitative analysis of literature, providing evidence-based strategies for prevention.Design This is a systematic review and meta-analysis, conducted following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.Data sources A comprehensive search strategy was implemented across eight electronic databases (PubMed, Embase, Web of Science, Cochrane Library, CNKI, VIP, Wanfang and CBM) from inception to February 2024.Eligibility criteria We included cross-sectional studies that measured serum 25-hydroxyvitamin D levels and analysed influencing factors (eg, age, season, region) in healthy children and adolescents aged 0–18 years in Mainland China; studies reporting prevalence data of vitamin D deficiency/insufficiency based on standardised thresholds (deficiency <50 nmol/L, insufficiency 50–75 nmol/L) and using validated detection methods (eg, liquid chromatography-tandem mass spectrometry, chemiluminescence immunoassay, ELISA) were prioritised.Data extraction and synthesis Two independent reviewers systematically searched, screened and extracted data using predefined protocols. Study quality was assessed with the Agency for Healthcare Research and Quality (AHRQ) tool. Meta-analyses were performed using random-effects models in Review Manager V.5.3 and Stata V.16.0, with subgroup analyses by age, season and region. Sensitivity analysis and Egger’s test were applied to evaluate robustness and publication bias. Findings were synthesised through narrative summaries and quantitative pooling.Results The pooled prevalence of vitamin D deficiency among children and adolescents in Mainland China was 48% (95% CI: 40% to 57%), with extreme heterogeneity across studies (I² = 99.98%, p<0.001). Sensitivity analysis confirmed the stability of pooled estimates. Subgroup analyses revealed significant age-related declines (infants: 82.35 nmol/L vs adolescents: 50.98 nmol/L, p<0.05). Study quality assessed by AHRQ criteria showed 16% of included studies were high-quality (scores 8-11/11), 84% moderate (4-7). Evidence of publication bias was detected via Egger’s test (p<0.05) and funnel plot asymmetry.Conclusions The study highlights the widespread nature of vitamin D deficiency among children and adolescents in Mainland China, particularly in older children and during winter months. Effective interventions are necessary to address this issue. Future research should prioritise methodological standardisation to reduce heterogeneity and address potential publication bias.PROSPERO registration number CRD42023479183.https://bmjopen.bmj.com/content/15/6/e094693.full
spellingShingle Bo Chen
Pengyun Ji
Zisheng Li
Wenyan Qin
Youqing She
Vitamin D levels and its influencing factors in children and adolescents in mainland China: a systematic review and meta-analysis
BMJ Open
title Vitamin D levels and its influencing factors in children and adolescents in mainland China: a systematic review and meta-analysis
title_full Vitamin D levels and its influencing factors in children and adolescents in mainland China: a systematic review and meta-analysis
title_fullStr Vitamin D levels and its influencing factors in children and adolescents in mainland China: a systematic review and meta-analysis
title_full_unstemmed Vitamin D levels and its influencing factors in children and adolescents in mainland China: a systematic review and meta-analysis
title_short Vitamin D levels and its influencing factors in children and adolescents in mainland China: a systematic review and meta-analysis
title_sort vitamin d levels and its influencing factors in children and adolescents in mainland china a systematic review and meta analysis
url https://bmjopen.bmj.com/content/15/6/e094693.full
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