An outbreak of Zika virus in western India in the metropolis of Pune in the monsoon of 2024

Background: Sporadic cases and rare outbreaks of Zika Virus (ZIKV) have been reported in India since 2016. Between June to early September 2024; the city of Pune in western India was affected with a large outbreak of ZIKV. Here we report the analysis of epidemiological; clinical and genomic characte...

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Main Authors: Gururaj Rao Deshpande, Gajanan N. Sapkal, Asha Salunke, Rashmi Gunjikar, Nitali Tadkalkar, Pradnya Shinde, Nidhi Daga, Manjusha Gopale, Ashwini Ramdasi, Supriya Hundekar, Kavita Lole, Rohan Raj Roy, Jose Antony Jenish, Rashi Srivastava, Shivani Parmar, Pooja Pawara, Kajal Jarande, Shankar Vidhate, Kirtee Khutwad
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Journal of Infection and Public Health
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Online Access:http://www.sciencedirect.com/science/article/pii/S1876034125000693
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author Gururaj Rao Deshpande
Gajanan N. Sapkal
Asha Salunke
Rashmi Gunjikar
Nitali Tadkalkar
Pradnya Shinde
Nidhi Daga
Manjusha Gopale
Ashwini Ramdasi
Supriya Hundekar
Kavita Lole
Rohan Raj Roy
Jose Antony Jenish
Rashi Srivastava
Shivani Parmar
Pooja Pawara
Kajal Jarande
Shankar Vidhate
Kirtee Khutwad
author_facet Gururaj Rao Deshpande
Gajanan N. Sapkal
Asha Salunke
Rashmi Gunjikar
Nitali Tadkalkar
Pradnya Shinde
Nidhi Daga
Manjusha Gopale
Ashwini Ramdasi
Supriya Hundekar
Kavita Lole
Rohan Raj Roy
Jose Antony Jenish
Rashi Srivastava
Shivani Parmar
Pooja Pawara
Kajal Jarande
Shankar Vidhate
Kirtee Khutwad
author_sort Gururaj Rao Deshpande
collection DOAJ
description Background: Sporadic cases and rare outbreaks of Zika Virus (ZIKV) have been reported in India since 2016. Between June to early September 2024; the city of Pune in western India was affected with a large outbreak of ZIKV. Here we report the analysis of epidemiological; clinical and genomic characteristics of the outbreak. Methods: Suspected ZIKV cases and pregnant women within 3 km radius of ZIKV positive cases and household contacts were included in the study. The study area included parts of the Pune metropolitan region. ZIKV RNA in serum and urine samples was detected by a single-plex Realtime RT-PCR assay. Representative samples were characterized by whole-genome sequencing. Results: Among clinically suspected ZIKV cases 63/433 (14.55 %); and 45/1615 (2.78 %) pregnant women surveyed; were found positive for acute ZIKV infection. Majority of positive cases were asymptomatic or had mild symptoms. Two cases reported complications of pyelonephritis and encephalitis respectively. There was no evidence of microcephaly in the fetuses of the pregnant women surveyed. The ZIKV strains were found to belong to the Asian lineage and clustered close to the Rajasthan strain reported previously in India. Mutation linked with microcephaly was not found in these sequences. Follow-up of pregnant women till October 2024 indicated 22 % (10/45) of women delivered healthy babies. Conclusion: The ZIKV strain associated with the present outbreak did not have mutations linked with microcephaly; but had mutations linked with enhanced transmission. Symptoms such as thrombocytopenia; bleeding manifestations; retro-orbital-pain; in a small subset of patients were unique; which were not reported in previous outbreaks in India. The findings highlight the urgent need for enhanced surveillance to plan strategies for public health measures to control the disease spread. Study highlights the need for monitoring the mutations and follow up of ZIKV infected pregnant women and their children to confirm absence of congenital anomalies.
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spelling doaj-art-99307f3b9c7d41539976cc418db4ca2a2025-08-20T02:09:40ZengElsevierJournal of Infection and Public Health1876-03412025-05-0118510272010.1016/j.jiph.2025.102720An outbreak of Zika virus in western India in the metropolis of Pune in the monsoon of 2024Gururaj Rao Deshpande0Gajanan N. Sapkal1Asha Salunke2Rashmi Gunjikar3Nitali Tadkalkar4Pradnya Shinde5Nidhi Daga6Manjusha Gopale7Ashwini Ramdasi8Supriya Hundekar9Kavita Lole10Rohan Raj Roy11Jose Antony Jenish12Rashi Srivastava13Shivani Parmar14Pooja Pawara15Kajal Jarande16Shankar Vidhate17Kirtee Khutwad18Diagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, India; Correspondence to: Diagnostic Virology Group, ICMR, National Institute of Virology, Pune, India.Diagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaHepatitis Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaHepatitis Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaHepatitis Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaDiagnostic Virology Group, ICMR-National Institute of Virology, MCC 130/1 Sus Road, Pashan, Pune, Maharashtra 411021, IndiaBackground: Sporadic cases and rare outbreaks of Zika Virus (ZIKV) have been reported in India since 2016. Between June to early September 2024; the city of Pune in western India was affected with a large outbreak of ZIKV. Here we report the analysis of epidemiological; clinical and genomic characteristics of the outbreak. Methods: Suspected ZIKV cases and pregnant women within 3 km radius of ZIKV positive cases and household contacts were included in the study. The study area included parts of the Pune metropolitan region. ZIKV RNA in serum and urine samples was detected by a single-plex Realtime RT-PCR assay. Representative samples were characterized by whole-genome sequencing. Results: Among clinically suspected ZIKV cases 63/433 (14.55 %); and 45/1615 (2.78 %) pregnant women surveyed; were found positive for acute ZIKV infection. Majority of positive cases were asymptomatic or had mild symptoms. Two cases reported complications of pyelonephritis and encephalitis respectively. There was no evidence of microcephaly in the fetuses of the pregnant women surveyed. The ZIKV strains were found to belong to the Asian lineage and clustered close to the Rajasthan strain reported previously in India. Mutation linked with microcephaly was not found in these sequences. Follow-up of pregnant women till October 2024 indicated 22 % (10/45) of women delivered healthy babies. Conclusion: The ZIKV strain associated with the present outbreak did not have mutations linked with microcephaly; but had mutations linked with enhanced transmission. Symptoms such as thrombocytopenia; bleeding manifestations; retro-orbital-pain; in a small subset of patients were unique; which were not reported in previous outbreaks in India. The findings highlight the urgent need for enhanced surveillance to plan strategies for public health measures to control the disease spread. Study highlights the need for monitoring the mutations and follow up of ZIKV infected pregnant women and their children to confirm absence of congenital anomalies.http://www.sciencedirect.com/science/article/pii/S1876034125000693Zika virusOutbreakFlavivirusesIndiaSurveillanceAsian lineage
spellingShingle Gururaj Rao Deshpande
Gajanan N. Sapkal
Asha Salunke
Rashmi Gunjikar
Nitali Tadkalkar
Pradnya Shinde
Nidhi Daga
Manjusha Gopale
Ashwini Ramdasi
Supriya Hundekar
Kavita Lole
Rohan Raj Roy
Jose Antony Jenish
Rashi Srivastava
Shivani Parmar
Pooja Pawara
Kajal Jarande
Shankar Vidhate
Kirtee Khutwad
An outbreak of Zika virus in western India in the metropolis of Pune in the monsoon of 2024
Journal of Infection and Public Health
Zika virus
Outbreak
Flaviviruses
India
Surveillance
Asian lineage
title An outbreak of Zika virus in western India in the metropolis of Pune in the monsoon of 2024
title_full An outbreak of Zika virus in western India in the metropolis of Pune in the monsoon of 2024
title_fullStr An outbreak of Zika virus in western India in the metropolis of Pune in the monsoon of 2024
title_full_unstemmed An outbreak of Zika virus in western India in the metropolis of Pune in the monsoon of 2024
title_short An outbreak of Zika virus in western India in the metropolis of Pune in the monsoon of 2024
title_sort outbreak of zika virus in western india in the metropolis of pune in the monsoon of 2024
topic Zika virus
Outbreak
Flaviviruses
India
Surveillance
Asian lineage
url http://www.sciencedirect.com/science/article/pii/S1876034125000693
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