miR-193a-3p prevents tumour progression by targeting TCL1 in diffuse large B-cell lymphoma
Abstract Both miR-193a-3p and T-Cell Leukemia/Lymphoma 1 (TCL1) are enriched in the PI3K/AKT signaling pathway, which is pivotal for the regulation of the initiation and progression of diffuse large B-cell lymphoma (DLBCL). Although miR-193a-3p has been demonstrated to exert an inhibitory effect in...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-025-06689-8 |
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| author | Haixia Gao Mengbo Wang Shuchen Xiong Ran Zhang Cancan Wang Huan Zhang Wenli Ji Cuicui Wang Zhiying Jia Xinxia Li |
| author_facet | Haixia Gao Mengbo Wang Shuchen Xiong Ran Zhang Cancan Wang Huan Zhang Wenli Ji Cuicui Wang Zhiying Jia Xinxia Li |
| author_sort | Haixia Gao |
| collection | DOAJ |
| description | Abstract Both miR-193a-3p and T-Cell Leukemia/Lymphoma 1 (TCL1) are enriched in the PI3K/AKT signaling pathway, which is pivotal for the regulation of the initiation and progression of diffuse large B-cell lymphoma (DLBCL). Although miR-193a-3p has been demonstrated to exert an inhibitory effect in various tumors, the relationship between miR-193a-3p and TCL1, as well as the mechanisms by which miR-193a-3p participates in the modulation of DLBCL onset and progression, remain to be elucidated. The expression levels of miR-193a-3p and TCL1 in DLBCL cells were examined using quantitative real-time polymerase chain reaction (qRT-PCR), and their impact on DLBCL cell growth was assessed through cell proliferation assays and flow cytometry. Subsequently, the interaction between miR-193a-3p and TCL1 was investigated using a dual-luciferase reporter assay. A nude mouse model of subcutaneous DLBCL was established in which the mice were injected with miR-193a-3p agomir, and were evaluated after 45 days. Finally, the mechanism by which miR-193a-3p modulates the PI3K/AKT signaling pathway was explored via qRT-PCR and Western blotting analyses. It was found that miR-193a-3p is downregulated, while TCL1 is upregulated in DLBCL. miR-193a-3p was shown to inhibit proliferation and induce apoptosis in DLBCL cells, in contrast to TCL1, which promotes proliferation and suppresses apoptosis. Moreover, TCL1 was identified as a target gene of miR-193a-3p. Additionally, it was demonstrated that miR-193a-3p regulates the PI3K/AKT signaling pathway through TCL1 both in vitro and in vivo. Collectively, these findings indicate that miR-193a-3p suppresses the growth of DLBCL by targeting TCL1 within the PI3K/AKT signaling pathway. |
| format | Article |
| id | doaj-art-992da6db48ec4f038b807fa3e2c19dd5 |
| institution | Kabale University |
| issn | 1479-5876 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-992da6db48ec4f038b807fa3e2c19dd52025-08-20T04:03:01ZengBMCJournal of Translational Medicine1479-58762025-07-0123111210.1186/s12967-025-06689-8miR-193a-3p prevents tumour progression by targeting TCL1 in diffuse large B-cell lymphomaHaixia Gao0Mengbo Wang1Shuchen Xiong2Ran Zhang3Cancan Wang4Huan Zhang5Wenli Ji6Cuicui Wang7Zhiying Jia8Xinxia Li9Departments of Pathology, The Affiliated Cancer Hospital of Xinjiang Medical UniversityDepartments of Ultrasound, The Affiliated Cancer Hospital of Xinjiang Medical UniversityDepartments of Pathology, The Affiliated Cancer Hospital of Xinjiang Medical UniversityDepartments of Pathology, The Affiliated Cancer Hospital of Xinjiang Medical UniversityDepartments of Pathology, The Affiliated Cancer Hospital of Xinjiang Medical UniversityDepartments of Pathology, The Affiliated Cancer Hospital of Xinjiang Medical UniversityDepartments of Pathology, The Affiliated Cancer Hospital of Xinjiang Medical UniversityDepartments of Pathology, The Affiliated Cancer Hospital of Xinjiang Medical UniversityDepartments of Ultrasound, The Affiliated Cancer Hospital of Xinjiang Medical UniversityDepartments of Pathology, The Affiliated Cancer Hospital of Xinjiang Medical UniversityAbstract Both miR-193a-3p and T-Cell Leukemia/Lymphoma 1 (TCL1) are enriched in the PI3K/AKT signaling pathway, which is pivotal for the regulation of the initiation and progression of diffuse large B-cell lymphoma (DLBCL). Although miR-193a-3p has been demonstrated to exert an inhibitory effect in various tumors, the relationship between miR-193a-3p and TCL1, as well as the mechanisms by which miR-193a-3p participates in the modulation of DLBCL onset and progression, remain to be elucidated. The expression levels of miR-193a-3p and TCL1 in DLBCL cells were examined using quantitative real-time polymerase chain reaction (qRT-PCR), and their impact on DLBCL cell growth was assessed through cell proliferation assays and flow cytometry. Subsequently, the interaction between miR-193a-3p and TCL1 was investigated using a dual-luciferase reporter assay. A nude mouse model of subcutaneous DLBCL was established in which the mice were injected with miR-193a-3p agomir, and were evaluated after 45 days. Finally, the mechanism by which miR-193a-3p modulates the PI3K/AKT signaling pathway was explored via qRT-PCR and Western blotting analyses. It was found that miR-193a-3p is downregulated, while TCL1 is upregulated in DLBCL. miR-193a-3p was shown to inhibit proliferation and induce apoptosis in DLBCL cells, in contrast to TCL1, which promotes proliferation and suppresses apoptosis. Moreover, TCL1 was identified as a target gene of miR-193a-3p. Additionally, it was demonstrated that miR-193a-3p regulates the PI3K/AKT signaling pathway through TCL1 both in vitro and in vivo. Collectively, these findings indicate that miR-193a-3p suppresses the growth of DLBCL by targeting TCL1 within the PI3K/AKT signaling pathway.https://doi.org/10.1186/s12967-025-06689-8miR-193a-3pTCL1PI3K/AKT signalling pathwayDiffuse large B-cell lymphoma |
| spellingShingle | Haixia Gao Mengbo Wang Shuchen Xiong Ran Zhang Cancan Wang Huan Zhang Wenli Ji Cuicui Wang Zhiying Jia Xinxia Li miR-193a-3p prevents tumour progression by targeting TCL1 in diffuse large B-cell lymphoma Journal of Translational Medicine miR-193a-3p TCL1 PI3K/AKT signalling pathway Diffuse large B-cell lymphoma |
| title | miR-193a-3p prevents tumour progression by targeting TCL1 in diffuse large B-cell lymphoma |
| title_full | miR-193a-3p prevents tumour progression by targeting TCL1 in diffuse large B-cell lymphoma |
| title_fullStr | miR-193a-3p prevents tumour progression by targeting TCL1 in diffuse large B-cell lymphoma |
| title_full_unstemmed | miR-193a-3p prevents tumour progression by targeting TCL1 in diffuse large B-cell lymphoma |
| title_short | miR-193a-3p prevents tumour progression by targeting TCL1 in diffuse large B-cell lymphoma |
| title_sort | mir 193a 3p prevents tumour progression by targeting tcl1 in diffuse large b cell lymphoma |
| topic | miR-193a-3p TCL1 PI3K/AKT signalling pathway Diffuse large B-cell lymphoma |
| url | https://doi.org/10.1186/s12967-025-06689-8 |
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