Evidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects: the melon fruit fly Zeugodacus cucurbitae (Diptera: Tephritidae) as a case study
Abstract Background The high invasiveness of phytophagous insects is related to their adaptability to various environments, that can be influenced by their associated microbial community. Microbial symbionts are known to play a key role in the biology, ecology, and evolution of phytophagous insects,...
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2024-12-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-024-03673-y |
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| author | Abir Hafsi Laura Moquet Wouter Hendrycks Marc De Meyer Massimiliano Virgilio Hélène Delatte |
| author_facet | Abir Hafsi Laura Moquet Wouter Hendrycks Marc De Meyer Massimiliano Virgilio Hélène Delatte |
| author_sort | Abir Hafsi |
| collection | DOAJ |
| description | Abstract Background The high invasiveness of phytophagous insects is related to their adaptability to various environments, that can be influenced by their associated microbial community. Microbial symbionts are known to play a key role in the biology, ecology, and evolution of phytophagous insects, but their abundance and diversity are suggested to be influenced by environmental stressors. In this work, using 16 S rRNA metabarcoding we aim to verify (1) if laboratory rearing affects microbial symbiont communities of Zeugodacus cucurbitae females, a cosmopolitan pest of cucurbitaceous crops (2) if temperature, diet quality, and antibiotic treatments affect microbial symbiont communities of both laboratory and wild populations, and (3) if changes in microbial symbiont communities due to temperature, diet and antibiotic affect longevity and fecundity of Z. cucurbitae. Results The results showed that microbial diversity, particularly the β-diversity was significantly affected by insect origin, temperature, diet quality, and antibiotic treatment. The alteration of gut microbial symbionts, specifically Enterobacteriaceae, was associated with low fecundity and longevity of Z. cucurbitae females feeding on optimal diet only. Fecundity reduction in antibiotic treated females was more pronounced when flies were fed on a poor diet without protein. Conclusions our study proves the relationship between gut microbiome and host fitness under thermal and diet fluctuation highlighting the importance of microbial community in the adaptation of Z. cucurbitae to environmental stress. Clinical trial number Not applicable. |
| format | Article |
| id | doaj-art-9929edfbe35b412b85724c122e4cce8d |
| institution | OA Journals |
| issn | 1471-2180 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-9929edfbe35b412b85724c122e4cce8d2025-08-20T02:31:02ZengBMCBMC Microbiology1471-21802024-12-0124111510.1186/s12866-024-03673-yEvidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects: the melon fruit fly Zeugodacus cucurbitae (Diptera: Tephritidae) as a case studyAbir Hafsi0Laura Moquet1Wouter Hendrycks2Marc De Meyer3Massimiliano Virgilio4Hélène Delatte5CIRAD, UMR PVBMTCIRAD, UMR PVBMTRoyal Museum for Central AfricaRoyal Museum for Central AfricaRoyal Museum for Central AfricaCIRAD, UMR PVBMTAbstract Background The high invasiveness of phytophagous insects is related to their adaptability to various environments, that can be influenced by their associated microbial community. Microbial symbionts are known to play a key role in the biology, ecology, and evolution of phytophagous insects, but their abundance and diversity are suggested to be influenced by environmental stressors. In this work, using 16 S rRNA metabarcoding we aim to verify (1) if laboratory rearing affects microbial symbiont communities of Zeugodacus cucurbitae females, a cosmopolitan pest of cucurbitaceous crops (2) if temperature, diet quality, and antibiotic treatments affect microbial symbiont communities of both laboratory and wild populations, and (3) if changes in microbial symbiont communities due to temperature, diet and antibiotic affect longevity and fecundity of Z. cucurbitae. Results The results showed that microbial diversity, particularly the β-diversity was significantly affected by insect origin, temperature, diet quality, and antibiotic treatment. The alteration of gut microbial symbionts, specifically Enterobacteriaceae, was associated with low fecundity and longevity of Z. cucurbitae females feeding on optimal diet only. Fecundity reduction in antibiotic treated females was more pronounced when flies were fed on a poor diet without protein. Conclusions our study proves the relationship between gut microbiome and host fitness under thermal and diet fluctuation highlighting the importance of microbial community in the adaptation of Z. cucurbitae to environmental stress. Clinical trial number Not applicable.https://doi.org/10.1186/s12866-024-03673-yInsect adaptationLongevityFecundityMicrobial symbiontMetabarcoding |
| spellingShingle | Abir Hafsi Laura Moquet Wouter Hendrycks Marc De Meyer Massimiliano Virgilio Hélène Delatte Evidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects: the melon fruit fly Zeugodacus cucurbitae (Diptera: Tephritidae) as a case study BMC Microbiology Insect adaptation Longevity Fecundity Microbial symbiont Metabarcoding |
| title | Evidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects: the melon fruit fly Zeugodacus cucurbitae (Diptera: Tephritidae) as a case study |
| title_full | Evidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects: the melon fruit fly Zeugodacus cucurbitae (Diptera: Tephritidae) as a case study |
| title_fullStr | Evidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects: the melon fruit fly Zeugodacus cucurbitae (Diptera: Tephritidae) as a case study |
| title_full_unstemmed | Evidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects: the melon fruit fly Zeugodacus cucurbitae (Diptera: Tephritidae) as a case study |
| title_short | Evidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects: the melon fruit fly Zeugodacus cucurbitae (Diptera: Tephritidae) as a case study |
| title_sort | evidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects the melon fruit fly zeugodacus cucurbitae diptera tephritidae as a case study |
| topic | Insect adaptation Longevity Fecundity Microbial symbiont Metabarcoding |
| url | https://doi.org/10.1186/s12866-024-03673-y |
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