Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria
Introduction: Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Ab...
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The Journal of Infection in Developing Countries
2015-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/5528 |
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| author | Casmir Ifeanyichukwu Cajetan Ifeanyi Nkiruka Florence Ikeneche Bassey Enya Bassey Nazek Al-Gallas Ridha Ben Aissa Abdellatif Boudabous |
| author_facet | Casmir Ifeanyichukwu Cajetan Ifeanyi Nkiruka Florence Ikeneche Bassey Enya Bassey Nazek Al-Gallas Ridha Ben Aissa Abdellatif Boudabous |
| author_sort | Casmir Ifeanyichukwu Cajetan Ifeanyi |
| collection | DOAJ |
| description | Introduction: Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria remains unknown.
Methodology: Stool specimens from 400 acute diarrheic children between 0 and 60 months of age were studied.E. coli strains isolated were evaluated by polymerase chain reaction (PCR) for nine virulence genes and HEp-2 cell adherence to detect and identify five distinct diarrheagenic E. coli categories.
Results: Diarrheagenic E.coli was detected in 51 (12.8%) of the diarrheic children. The observed DEC pathotypes were enteropathogenic E. coli (EPEC) in 18 (4.5%) children, enterotoxigenic E. coli (ETEC) in 16 (4.0%), enteroaggrative E. coli (EAEC) in 8 (2.0%), enterohaemorrhagic E. coli (EHEC) in 6 (1.5%), and enteroinvasive E. coli (EIEC) in 3 (0.8%). Four (1.0 %) EPEC strains with only the eae+ gene that adhered diffusely to HEp-2 cell were identified as atypical EPEC. All the DEC categories except atypical EPEC were identified in children between 6 and 12 months of age.
Conclusions: This study underscores the need for routine evaluation of diarrheic children for virulence properties of infectious DEC. Atypical EPEC are emerging among the DEC pathotypes isolated from childhood acute gastroenteritis in Abuja, Nigeria.
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| format | Article |
| id | doaj-art-991a14b2c8a6472a91ae486d6a219c83 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-991a14b2c8a6472a91ae486d6a219c832025-08-20T03:48:46ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-02-0190210.3855/jidc.5528Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, NigeriaCasmir Ifeanyichukwu Cajetan Ifeanyi0Nkiruka Florence Ikeneche1Bassey Enya Bassey2Nazek Al-Gallas3Ridha Ben Aissa4Abdellatif Boudabous5Faculty of Veterinary Medicine, University of Abuja, Abuja, NigeriaCollege of Health Science, University of Abuja, Abuja, Nigeria 3 World Health Organization Office, Abuja, NigeriaLaboratoire de Controle des Eaux et Denrées Alimentaires, Institut Pasteur de Tunis, TunisiaLaboratoire de Controle des Eaux et Denre´es Alimentaires, Institut Pasteur de Tunis, TunisiaLaboratoire de Controle des Eaux et Denrées Alimentaires, Institut Pasteur de Tunis, TunisiaLaboratoire de Microbiologie, Faculté des Sciences deTunis, Tunis, TunisiaIntroduction: Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria remains unknown. Methodology: Stool specimens from 400 acute diarrheic children between 0 and 60 months of age were studied.E. coli strains isolated were evaluated by polymerase chain reaction (PCR) for nine virulence genes and HEp-2 cell adherence to detect and identify five distinct diarrheagenic E. coli categories. Results: Diarrheagenic E.coli was detected in 51 (12.8%) of the diarrheic children. The observed DEC pathotypes were enteropathogenic E. coli (EPEC) in 18 (4.5%) children, enterotoxigenic E. coli (ETEC) in 16 (4.0%), enteroaggrative E. coli (EAEC) in 8 (2.0%), enterohaemorrhagic E. coli (EHEC) in 6 (1.5%), and enteroinvasive E. coli (EIEC) in 3 (0.8%). Four (1.0 %) EPEC strains with only the eae+ gene that adhered diffusely to HEp-2 cell were identified as atypical EPEC. All the DEC categories except atypical EPEC were identified in children between 6 and 12 months of age. Conclusions: This study underscores the need for routine evaluation of diarrheic children for virulence properties of infectious DEC. Atypical EPEC are emerging among the DEC pathotypes isolated from childhood acute gastroenteritis in Abuja, Nigeria. https://jidc.org/index.php/journal/article/view/5528diarrheagenicvirulence genespathotype |
| spellingShingle | Casmir Ifeanyichukwu Cajetan Ifeanyi Nkiruka Florence Ikeneche Bassey Enya Bassey Nazek Al-Gallas Ridha Ben Aissa Abdellatif Boudabous Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria Journal of Infection in Developing Countries diarrheagenic virulence genes pathotype |
| title | Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria |
| title_full | Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria |
| title_fullStr | Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria |
| title_full_unstemmed | Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria |
| title_short | Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria |
| title_sort | diarrheagenic escherichia coli pathotypes isolated from children with diarrhea in the federal capital territory abuja nigeria |
| topic | diarrheagenic virulence genes pathotype |
| url | https://jidc.org/index.php/journal/article/view/5528 |
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