SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice
Gut dysbiosis is associated with several pathological processes. Previous study showed that regular exercise can protect against dysmetabolism in high-fat diet (HFD) fed mice through butyrate-SESN2 pathway, and SESN2 ablation weakened the protective effects of exercise. Here, we investigated whether...
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000563 |
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| author | Chunxia Yu Peng Zhang Sujuan Liu Yanmei Niu Li Fu |
| author_facet | Chunxia Yu Peng Zhang Sujuan Liu Yanmei Niu Li Fu |
| author_sort | Chunxia Yu |
| collection | DOAJ |
| description | Gut dysbiosis is associated with several pathological processes. Previous study showed that regular exercise can protect against dysmetabolism in high-fat diet (HFD) fed mice through butyrate-SESN2 pathway, and SESN2 ablation weakened the protective effects of exercise. Here, we investigated whether SESN2-deficiency suppresses the exercise response to microbiota composition and subsequently reduces the benefits of exercise on dysmetabolism induced by HFD. Wild type (WT) and SESN2−/− mice were assigned to five-groups, fed with either normal chow or HFD and with or without exercise training for 15-week. Fecal microbiota composition and function were assessed by 16S rRNA sequencing. The sequencing results showed that SESN2−/− mice displayed differed microbiome profile from WT mice. Exercise enriched the microflora diversity and increased the beneficial microbial species in WT mice, and SESN2 ablation weakened the beneficial effects of exercise on microbial resilience following HFD consumption. Moreover, network analysis revealed that exercise increased correlation density and clustering of operational taxonomic units in WT mice only. KEGG demonstrated that some dominant metabolism-related enzymes and modules increased in SESN2−/− mice. Our results indicated that the effects of exercise on metabolism are associated with the perturbations of gut microbiota composition and function, suggesting that SESN2 contributes to maintain metabolic homeostasis.− |
| format | Article |
| id | doaj-art-990cc8bd70c343768dac9f34918c9ec9 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-990cc8bd70c343768dac9f34918c9ec92025-02-03T02:42:14ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112619611968SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in miceChunxia Yu0Peng Zhang1Sujuan Liu2Yanmei Niu3Li Fu4Department of Physiology and Pathophysiology, School of Basic Medical Science, Tianjin Medical University, Tianjin 300070, ChinaDepartment of Rehabilitation, School of Medical Technology, Tianjin Medical University, Tianjin 300070, ChinaDepartment of Anatomy and Histology, School of Basic Medical Science, Tianjin Medical University, Tianjin 300070, ChinaDepartment of Rehabilitation, School of Medical Technology, Tianjin Medical University, Tianjin 300070, ChinaDepartment of Physiology and Pathophysiology, School of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China; Department of Rehabilitation, School of Medical Technology, Tianjin Medical University, Tianjin 300070, China; Corresponding author at: Department of Physiology and Pathophysiology, School of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China.Gut dysbiosis is associated with several pathological processes. Previous study showed that regular exercise can protect against dysmetabolism in high-fat diet (HFD) fed mice through butyrate-SESN2 pathway, and SESN2 ablation weakened the protective effects of exercise. Here, we investigated whether SESN2-deficiency suppresses the exercise response to microbiota composition and subsequently reduces the benefits of exercise on dysmetabolism induced by HFD. Wild type (WT) and SESN2−/− mice were assigned to five-groups, fed with either normal chow or HFD and with or without exercise training for 15-week. Fecal microbiota composition and function were assessed by 16S rRNA sequencing. The sequencing results showed that SESN2−/− mice displayed differed microbiome profile from WT mice. Exercise enriched the microflora diversity and increased the beneficial microbial species in WT mice, and SESN2 ablation weakened the beneficial effects of exercise on microbial resilience following HFD consumption. Moreover, network analysis revealed that exercise increased correlation density and clustering of operational taxonomic units in WT mice only. KEGG demonstrated that some dominant metabolism-related enzymes and modules increased in SESN2−/− mice. Our results indicated that the effects of exercise on metabolism are associated with the perturbations of gut microbiota composition and function, suggesting that SESN2 contributes to maintain metabolic homeostasis.−http://www.sciencedirect.com/science/article/pii/S2213453023000563ExerciseHigh-fat dietSestrin2ObesityGut microbiota |
| spellingShingle | Chunxia Yu Peng Zhang Sujuan Liu Yanmei Niu Li Fu SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice Food Science and Human Wellness Exercise High-fat diet Sestrin2 Obesity Gut microbiota |
| title | SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice |
| title_full | SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice |
| title_fullStr | SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice |
| title_full_unstemmed | SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice |
| title_short | SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice |
| title_sort | sesn2 ablation weakens exercise benefits on resilience of gut microbiota following high fat diet consumption in mice |
| topic | Exercise High-fat diet Sestrin2 Obesity Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000563 |
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