RNA Sequencing Revealed a Weak Response of Gingival Fibroblasts Exposed to Hyaluronic Acid
Hyaluronic acid was proposed to support soft tissue recession surgery and guided tissue regeneration. The molecular mechanisms through which hyaluronic acid modulates the response of connective tissue cells remain elusive. To elucidate the impact of hyaluronic acid on the connective tissue cells, we...
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MDPI AG
2024-12-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/11/12/1307 |
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| author | Layla Panahipour Atefe Imani Natália dos Santos Sanches Hannes Kühtreiber Michael Mildner Reinhard Gruber |
| author_facet | Layla Panahipour Atefe Imani Natália dos Santos Sanches Hannes Kühtreiber Michael Mildner Reinhard Gruber |
| author_sort | Layla Panahipour |
| collection | DOAJ |
| description | Hyaluronic acid was proposed to support soft tissue recession surgery and guided tissue regeneration. The molecular mechanisms through which hyaluronic acid modulates the response of connective tissue cells remain elusive. To elucidate the impact of hyaluronic acid on the connective tissue cells, we used bulk RNA sequencing to determine the changes in the genetic signature of gingival fibroblasts exposed to 1.6% cross-linked hyaluronic acid and 0.2% natural hyaluronic acid. Transcriptome-wide changes were modest. Even when implementing a minimum of 1.5 log2 fold-change and a significance threshold of 1.0 −log10, only a dozenth of genes were differentially expressed. Upregulated genes were PLK3, SLC16A6, IL6, HBEGF, DGKE, DUSP4, PTGS2, FOXC2, ATAD2B, NFATC2, and downregulated genes were MMP24 and PLXNA2. RT-PCR analysis supported the impact of hyaluronic acid on increasing the expression of a selected gene panel. The findings from bulk RNA sequencing suggest that gingival fibroblasts experience weak changes in their transcriptome when exposed to hyaluronic acid. |
| format | Article |
| id | doaj-art-990601332aab438eaa13535d232bf712 |
| institution | DOAJ |
| issn | 2306-5354 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Bioengineering |
| spelling | doaj-art-990601332aab438eaa13535d232bf7122025-08-20T02:53:18ZengMDPI AGBioengineering2306-53542024-12-011112130710.3390/bioengineering11121307RNA Sequencing Revealed a Weak Response of Gingival Fibroblasts Exposed to Hyaluronic AcidLayla Panahipour0Atefe Imani1Natália dos Santos Sanches2Hannes Kühtreiber3Michael Mildner4Reinhard Gruber5Department of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, Sensengasse 2a, 1090 Vienna, AustriaDepartment of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, Sensengasse 2a, 1090 Vienna, AustriaDepartment of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, Sensengasse 2a, 1090 Vienna, AustriaDepartment of Dermatology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Dermatology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, Sensengasse 2a, 1090 Vienna, AustriaHyaluronic acid was proposed to support soft tissue recession surgery and guided tissue regeneration. The molecular mechanisms through which hyaluronic acid modulates the response of connective tissue cells remain elusive. To elucidate the impact of hyaluronic acid on the connective tissue cells, we used bulk RNA sequencing to determine the changes in the genetic signature of gingival fibroblasts exposed to 1.6% cross-linked hyaluronic acid and 0.2% natural hyaluronic acid. Transcriptome-wide changes were modest. Even when implementing a minimum of 1.5 log2 fold-change and a significance threshold of 1.0 −log10, only a dozenth of genes were differentially expressed. Upregulated genes were PLK3, SLC16A6, IL6, HBEGF, DGKE, DUSP4, PTGS2, FOXC2, ATAD2B, NFATC2, and downregulated genes were MMP24 and PLXNA2. RT-PCR analysis supported the impact of hyaluronic acid on increasing the expression of a selected gene panel. The findings from bulk RNA sequencing suggest that gingival fibroblasts experience weak changes in their transcriptome when exposed to hyaluronic acid.https://www.mdpi.com/2306-5354/11/12/1307hyaluronic acidgingival fibroblastsmacrophagesbulk RNAseqguided bone regenerationsoft tissue augmentation |
| spellingShingle | Layla Panahipour Atefe Imani Natália dos Santos Sanches Hannes Kühtreiber Michael Mildner Reinhard Gruber RNA Sequencing Revealed a Weak Response of Gingival Fibroblasts Exposed to Hyaluronic Acid Bioengineering hyaluronic acid gingival fibroblasts macrophages bulk RNAseq guided bone regeneration soft tissue augmentation |
| title | RNA Sequencing Revealed a Weak Response of Gingival Fibroblasts Exposed to Hyaluronic Acid |
| title_full | RNA Sequencing Revealed a Weak Response of Gingival Fibroblasts Exposed to Hyaluronic Acid |
| title_fullStr | RNA Sequencing Revealed a Weak Response of Gingival Fibroblasts Exposed to Hyaluronic Acid |
| title_full_unstemmed | RNA Sequencing Revealed a Weak Response of Gingival Fibroblasts Exposed to Hyaluronic Acid |
| title_short | RNA Sequencing Revealed a Weak Response of Gingival Fibroblasts Exposed to Hyaluronic Acid |
| title_sort | rna sequencing revealed a weak response of gingival fibroblasts exposed to hyaluronic acid |
| topic | hyaluronic acid gingival fibroblasts macrophages bulk RNAseq guided bone regeneration soft tissue augmentation |
| url | https://www.mdpi.com/2306-5354/11/12/1307 |
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