The Quantitative Analysis of bFGF and VEGF by ELISA in Human Meningiomas
The quantitative analysis of VEGF using ELISA in various subtypes of grade I meningiomas reported higher VEGF contents in meningothelial (2.38±0.62 pg/μg protein, n=7), transitional (1.08±0.21 pg/μg protein, n=13), and microcystic meningiomas (1.98±0.87 pg/μg protein, n=5) as compared with fibrous o...
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| Format: | Article |
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Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/36376 |
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| author | Yves Denizot Rafael De Armas François Caire Jean Jacques Moreau Isabelle Pommepuy Véronique Truffinet François Labrousse |
| author_facet | Yves Denizot Rafael De Armas François Caire Jean Jacques Moreau Isabelle Pommepuy Véronique Truffinet François Labrousse |
| author_sort | Yves Denizot |
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| description | The quantitative analysis of VEGF using ELISA in various subtypes of grade I meningiomas reported higher VEGF contents in meningothelial (2.38±0.62 pg/μg protein, n=7), transitional (1.08±0.21 pg/μg protein, n=13), and microcystic meningiomas (1.98±0.87 pg/μg protein, n=5) as compared with fibrous ones (0.36±0.09 pg/μg protein, n=5). In contrast to VEGF, no difference in the concentrations of bFGF was detected. VEGF levels did not correlate with meningioma grade (1.47±0.23 pg/μg versus 2.29±0.58 pg/μg for 32 and 16 grade I and II, resp), vascularisation (1.53±0.41 pg/μg versus 1.96±0.28 pg/μg for 24 low and 24 high vascularisated tumours, resp), and brain invasion (2.32±0.59 pg/μg versus 1.46±0.27 pg/μg for 7 and 41 patients with and without invasion, resp). The ELISA procedure is, thus, an interesting tool to ensure VEGF and bFGF levels in meningiomas and to test putative correlations with clinical parameters. It is, thus, tempting to speculate that ELISA would also be valuable for the quantitative analysis of other angiogenic growth factors and cytokines in intracranial tumours. |
| format | Article |
| id | doaj-art-9901b4f254e3461b8d30e97b45dd897f |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-9901b4f254e3461b8d30e97b45dd897f2025-02-03T01:27:47ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/3637636376The Quantitative Analysis of bFGF and VEGF by ELISA in Human MeningiomasYves Denizot0Rafael De Armas1François Caire2Jean Jacques Moreau3Isabelle Pommepuy4Véronique Truffinet5François Labrousse6UMR CNRS 6101, 2 rue du Dr. Marcland, Limoges 87025, FranceService d'Anatomie Pathologique, CHU Dupuytren, 2 Avenue M. Luther King, Limoges 87045, FranceService de Neurosurgery, CHU Dupuytren, 2 Avenue M. Luther King, Limoges 87045, FranceService de Neurosurgery, CHU Dupuytren, 2 Avenue M. Luther King, Limoges 87045, FranceService d'Anatomie Pathologique, CHU Dupuytren, 2 Avenue M. Luther King, Limoges 87045, FranceUMR CNRS 6101, 2 rue du Dr. Marcland, Limoges 87025, FranceService d'Anatomie Pathologique, CHU Dupuytren, 2 Avenue M. Luther King, Limoges 87045, FranceThe quantitative analysis of VEGF using ELISA in various subtypes of grade I meningiomas reported higher VEGF contents in meningothelial (2.38±0.62 pg/μg protein, n=7), transitional (1.08±0.21 pg/μg protein, n=13), and microcystic meningiomas (1.98±0.87 pg/μg protein, n=5) as compared with fibrous ones (0.36±0.09 pg/μg protein, n=5). In contrast to VEGF, no difference in the concentrations of bFGF was detected. VEGF levels did not correlate with meningioma grade (1.47±0.23 pg/μg versus 2.29±0.58 pg/μg for 32 and 16 grade I and II, resp), vascularisation (1.53±0.41 pg/μg versus 1.96±0.28 pg/μg for 24 low and 24 high vascularisated tumours, resp), and brain invasion (2.32±0.59 pg/μg versus 1.46±0.27 pg/μg for 7 and 41 patients with and without invasion, resp). The ELISA procedure is, thus, an interesting tool to ensure VEGF and bFGF levels in meningiomas and to test putative correlations with clinical parameters. It is, thus, tempting to speculate that ELISA would also be valuable for the quantitative analysis of other angiogenic growth factors and cytokines in intracranial tumours.http://dx.doi.org/10.1155/MI/2006/36376 |
| spellingShingle | Yves Denizot Rafael De Armas François Caire Jean Jacques Moreau Isabelle Pommepuy Véronique Truffinet François Labrousse The Quantitative Analysis of bFGF and VEGF by ELISA in Human Meningiomas Mediators of Inflammation |
| title | The Quantitative Analysis of bFGF and VEGF by ELISA in Human Meningiomas |
| title_full | The Quantitative Analysis of bFGF and VEGF by ELISA in Human Meningiomas |
| title_fullStr | The Quantitative Analysis of bFGF and VEGF by ELISA in Human Meningiomas |
| title_full_unstemmed | The Quantitative Analysis of bFGF and VEGF by ELISA in Human Meningiomas |
| title_short | The Quantitative Analysis of bFGF and VEGF by ELISA in Human Meningiomas |
| title_sort | quantitative analysis of bfgf and vegf by elisa in human meningiomas |
| url | http://dx.doi.org/10.1155/MI/2006/36376 |
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