Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores.
Growing evidences indicate that proteases are implicated in adipogenesis and in the onset of obesity. We previously reported that the cysteine protease cathepsin K (ctsk) is overexpressed in the white adipose tissue (WAT) of obese individuals. We herein characterized the WAT and the metabolic phenot...
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Public Library of Science (PLoS)
2007-08-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0000683&type=printable |
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| author | Marcella Funicello Michela Novelli Maurizio Ragni Teresa Vottari Cesare Cocuzza Joaquin Soriano-Lopez Chiara Chiellini Federico Boschi Pasquina Marzola Pellegrino Masiello Paul Saftig Ferruccio Santini Rene St-Jacques Sylvie Desmarais Nicolas Morin Joseph Mancini M David Percival Aldo Pinchera Margherita Maffei |
| author_facet | Marcella Funicello Michela Novelli Maurizio Ragni Teresa Vottari Cesare Cocuzza Joaquin Soriano-Lopez Chiara Chiellini Federico Boschi Pasquina Marzola Pellegrino Masiello Paul Saftig Ferruccio Santini Rene St-Jacques Sylvie Desmarais Nicolas Morin Joseph Mancini M David Percival Aldo Pinchera Margherita Maffei |
| author_sort | Marcella Funicello |
| collection | DOAJ |
| description | Growing evidences indicate that proteases are implicated in adipogenesis and in the onset of obesity. We previously reported that the cysteine protease cathepsin K (ctsk) is overexpressed in the white adipose tissue (WAT) of obese individuals. We herein characterized the WAT and the metabolic phenotype of ctsk deficient animals (ctsk-/-). When the growth rate of ctsk-/- was compared to that of the wild type animals (WT), we could establish a time window (5-8 weeks of age) within which ctsk-/-display significantly lower body weight and WAT size as compared to WT. Such a difference was not observable in older mice. Upon treatment with high fat diet (HFD) for 12 weeks ctsk-/- gained significantly less weight than WT and showed reduced brown adipose tissue, liver mass and a lower percentage of body fat. Plasma triglycerides, cholesterol and leptin were significantly lower in HFD-fed-ctsk-/- as compared to HFD-fed WT animals. Adipocyte lipolysis rates were increased in both young and HFD-fed-ctsk-/-, as compared to WT. Carnitine palmitoyl transferase-1 activity, was higher in mitochondria isolated from the WAT of HFD treated ctsk-/- as compared to WT. Together, these data indicate that ctsk ablation in mice results in reduced body fat content under conditions requiring a rapid accumulation of fat stores. This observation could be partly explained by an increased release and/or utilization of FFA and by an augmented ratio of lipolysis/lipogenesis. These results also demonstrate that under a HFD, ctsk deficiency confers a partial resistance to the development of dyslipidemia. |
| format | Article |
| id | doaj-art-9900b82b32b749e2925a56a8a5d4331c |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2007-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-9900b82b32b749e2925a56a8a5d4331c2025-08-20T02:00:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-08-0128e68310.1371/journal.pone.0000683Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores.Marcella FunicelloMichela NovelliMaurizio RagniTeresa VottariCesare CocuzzaJoaquin Soriano-LopezChiara ChielliniFederico BoschiPasquina MarzolaPellegrino MasielloPaul SaftigFerruccio SantiniRene St-JacquesSylvie DesmaraisNicolas MorinJoseph ManciniM David PercivalAldo PincheraMargherita MaffeiGrowing evidences indicate that proteases are implicated in adipogenesis and in the onset of obesity. We previously reported that the cysteine protease cathepsin K (ctsk) is overexpressed in the white adipose tissue (WAT) of obese individuals. We herein characterized the WAT and the metabolic phenotype of ctsk deficient animals (ctsk-/-). When the growth rate of ctsk-/- was compared to that of the wild type animals (WT), we could establish a time window (5-8 weeks of age) within which ctsk-/-display significantly lower body weight and WAT size as compared to WT. Such a difference was not observable in older mice. Upon treatment with high fat diet (HFD) for 12 weeks ctsk-/- gained significantly less weight than WT and showed reduced brown adipose tissue, liver mass and a lower percentage of body fat. Plasma triglycerides, cholesterol and leptin were significantly lower in HFD-fed-ctsk-/- as compared to HFD-fed WT animals. Adipocyte lipolysis rates were increased in both young and HFD-fed-ctsk-/-, as compared to WT. Carnitine palmitoyl transferase-1 activity, was higher in mitochondria isolated from the WAT of HFD treated ctsk-/- as compared to WT. Together, these data indicate that ctsk ablation in mice results in reduced body fat content under conditions requiring a rapid accumulation of fat stores. This observation could be partly explained by an increased release and/or utilization of FFA and by an augmented ratio of lipolysis/lipogenesis. These results also demonstrate that under a HFD, ctsk deficiency confers a partial resistance to the development of dyslipidemia.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0000683&type=printable |
| spellingShingle | Marcella Funicello Michela Novelli Maurizio Ragni Teresa Vottari Cesare Cocuzza Joaquin Soriano-Lopez Chiara Chiellini Federico Boschi Pasquina Marzola Pellegrino Masiello Paul Saftig Ferruccio Santini Rene St-Jacques Sylvie Desmarais Nicolas Morin Joseph Mancini M David Percival Aldo Pinchera Margherita Maffei Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores. PLoS ONE |
| title | Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores. |
| title_full | Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores. |
| title_fullStr | Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores. |
| title_full_unstemmed | Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores. |
| title_short | Cathepsin K null mice show reduced adiposity during the rapid accumulation of fat stores. |
| title_sort | cathepsin k null mice show reduced adiposity during the rapid accumulation of fat stores |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0000683&type=printable |
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