The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding
Mitochondrial transcript maturation in African trypanosomes requires RNA editing to convert sequence-deficient pre-mRNAs into translatable mRNAs. The different pre-mRNAs have been shown to adopt highly stable 2D folds; however, it is not known whether these structures resemble the in vivo folds give...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2017/6067345 |
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| author | W.-Matthias Leeder Stephan Voskuhl H. Ulrich Göringer |
| author_facet | W.-Matthias Leeder Stephan Voskuhl H. Ulrich Göringer |
| author_sort | W.-Matthias Leeder |
| collection | DOAJ |
| description | Mitochondrial transcript maturation in African trypanosomes requires RNA editing to convert sequence-deficient pre-mRNAs into translatable mRNAs. The different pre-mRNAs have been shown to adopt highly stable 2D folds; however, it is not known whether these structures resemble the in vivo folds given the extreme “crowding” conditions within the mitochondrion. Here, we analyze the effects of macromolecular crowding on the structure of the mitochondrial RPS12 pre-mRNA. We use high molecular mass polyethylene glycol as a macromolecular cosolute and monitor the structure of the RNA globally and with nucleotide resolution. We demonstrate that crowding has no impact on the 2D fold and we conclude that the MFE structure in dilute solvent conditions represents a good proxy for the folding of the pre-mRNA in its mitochondrial solvent context. |
| format | Article |
| id | doaj-art-98fe1d6634974ff6a29b328cfff43229 |
| institution | OA Journals |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-98fe1d6634974ff6a29b328cfff432292025-08-20T02:20:25ZengWileyJournal of Nucleic Acids2090-02012090-021X2017-01-01201710.1155/2017/60673456067345The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular CrowdingW.-Matthias Leeder0Stephan Voskuhl1H. Ulrich Göringer2Department of Molecular Genetics, Darmstadt University of Technology, Darmstadt, GermanyDepartment of Molecular Genetics, Darmstadt University of Technology, Darmstadt, GermanyDepartment of Molecular Genetics, Darmstadt University of Technology, Darmstadt, GermanyMitochondrial transcript maturation in African trypanosomes requires RNA editing to convert sequence-deficient pre-mRNAs into translatable mRNAs. The different pre-mRNAs have been shown to adopt highly stable 2D folds; however, it is not known whether these structures resemble the in vivo folds given the extreme “crowding” conditions within the mitochondrion. Here, we analyze the effects of macromolecular crowding on the structure of the mitochondrial RPS12 pre-mRNA. We use high molecular mass polyethylene glycol as a macromolecular cosolute and monitor the structure of the RNA globally and with nucleotide resolution. We demonstrate that crowding has no impact on the 2D fold and we conclude that the MFE structure in dilute solvent conditions represents a good proxy for the folding of the pre-mRNA in its mitochondrial solvent context.http://dx.doi.org/10.1155/2017/6067345 |
| spellingShingle | W.-Matthias Leeder Stephan Voskuhl H. Ulrich Göringer The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding Journal of Nucleic Acids |
| title | The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding |
| title_full | The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding |
| title_fullStr | The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding |
| title_full_unstemmed | The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding |
| title_short | The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding |
| title_sort | 2d structure of the t brucei preedited rps12 mrna is not affected by macromolecular crowding |
| url | http://dx.doi.org/10.1155/2017/6067345 |
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