MiR-26a downregulates retinoblastoma in colorectal cancer
MicroRNAs are non-coding short RNAs that target the 3′ untranslated region of messenger RNAs (mRNAs) and lead to their degradation or to translational repression. Several microRNAs have been designated as oncomirs, owing to their regulating tumor suppressor genes. Interestingly, a few of them have b...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-04-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317695945 |
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| author | Eduardo López-Urrutia Jossimar Coronel-Hernández Verónica García-Castillo Carlos Contreras-Romero Antonio Martínez-Gutierrez Diana Estrada-Galicia Luis Ignacio Terrazas César López-Camarillo Hector Maldonado-Martínez Nadia Jacobo-Herrera Carlos Pérez-Plasencia |
| author_facet | Eduardo López-Urrutia Jossimar Coronel-Hernández Verónica García-Castillo Carlos Contreras-Romero Antonio Martínez-Gutierrez Diana Estrada-Galicia Luis Ignacio Terrazas César López-Camarillo Hector Maldonado-Martínez Nadia Jacobo-Herrera Carlos Pérez-Plasencia |
| author_sort | Eduardo López-Urrutia |
| collection | DOAJ |
| description | MicroRNAs are non-coding short RNAs that target the 3′ untranslated region of messenger RNAs (mRNAs) and lead to their degradation or to translational repression. Several microRNAs have been designated as oncomirs, owing to their regulating tumor suppressor genes. Interestingly, a few of them have been found to target multiple genes whose simultaneous suppression contributes to the development of a tumoral phenotype. Here, we have showed that miR-26a is overexpressed in colorectal cancer data obtained from TCGA Research Network and in human colon cancer pathological specimens; moreover, an orthotopic in vivo model of colon cancer showed overexpression of miR-26a, while Rb1 expression inversely correlated to miR-26a in TCGA Research Network data, pathological samples, and the in vivo model. Then, by means of luciferase assay, we demonstrated that miR-26a targets the 3′ untranslated region of Rb1 mRNA directly. This is, to our knowledge, the first report of miR-26a targeting Rb1 in colon cancer. The results of this study suggested that miR-26a could serve as a progression biomarker in colorectal cancer. Further validation studies are still needed to confirm our findings. |
| format | Article |
| id | doaj-art-98f3c193073c42d7ae459d5ec417984f |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-98f3c193073c42d7ae459d5ec417984f2025-08-20T02:52:09ZengSAGE PublishingTumor Biology1423-03802017-04-013910.1177/1010428317695945MiR-26a downregulates retinoblastoma in colorectal cancerEduardo López-Urrutia0Jossimar Coronel-Hernández1Verónica García-Castillo2Carlos Contreras-Romero3Antonio Martínez-Gutierrez4Diana Estrada-Galicia5Luis Ignacio Terrazas6César López-Camarillo7Hector Maldonado-Martínez8Nadia Jacobo-Herrera9Carlos Pérez-Plasencia10Laboratorio de Genómica Funcional, Unidad de Biomedicina, Facultad de Estudios Superiores (FES) Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla, MéxicoLaboratorio de Genómica Funcional, Unidad de Biomedicina, Facultad de Estudios Superiores (FES) Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla, MéxicoLaboratorio de Genómica Funcional, Unidad de Biomedicina, Facultad de Estudios Superiores (FES) Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla, MéxicoLaboratorio de Genómica Funcional, Unidad de Biomedicina, Facultad de Estudios Superiores (FES) Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla, MéxicoLaboratorio de Genómica Funcional, Unidad de Biomedicina, Facultad de Estudios Superiores (FES) Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla, MéxicoLaboratorio de Genómica Funcional, Unidad de Biomedicina, Facultad de Estudios Superiores (FES) Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla, MéxicoLaboratorio de Inmunología, Facultad de Estudios Superiores (FES) Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla, MexicoPosgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Mexico City, MexicoDirección de Patología, Instituto Nacional de Cancerología, Tlalpan, MéxicoUnidad de Bioquímica, Instituto Nacional De Ciencias Médicas Y Nutrición Salvador Zubirán, Tlalpan, MexicoLaboratorio de Genómica, Instituto Nacional de Cancerología, Tlalpan, MéxicoMicroRNAs are non-coding short RNAs that target the 3′ untranslated region of messenger RNAs (mRNAs) and lead to their degradation or to translational repression. Several microRNAs have been designated as oncomirs, owing to their regulating tumor suppressor genes. Interestingly, a few of them have been found to target multiple genes whose simultaneous suppression contributes to the development of a tumoral phenotype. Here, we have showed that miR-26a is overexpressed in colorectal cancer data obtained from TCGA Research Network and in human colon cancer pathological specimens; moreover, an orthotopic in vivo model of colon cancer showed overexpression of miR-26a, while Rb1 expression inversely correlated to miR-26a in TCGA Research Network data, pathological samples, and the in vivo model. Then, by means of luciferase assay, we demonstrated that miR-26a targets the 3′ untranslated region of Rb1 mRNA directly. This is, to our knowledge, the first report of miR-26a targeting Rb1 in colon cancer. The results of this study suggested that miR-26a could serve as a progression biomarker in colorectal cancer. Further validation studies are still needed to confirm our findings.https://doi.org/10.1177/1010428317695945 |
| spellingShingle | Eduardo López-Urrutia Jossimar Coronel-Hernández Verónica García-Castillo Carlos Contreras-Romero Antonio Martínez-Gutierrez Diana Estrada-Galicia Luis Ignacio Terrazas César López-Camarillo Hector Maldonado-Martínez Nadia Jacobo-Herrera Carlos Pérez-Plasencia MiR-26a downregulates retinoblastoma in colorectal cancer Tumor Biology |
| title | MiR-26a downregulates retinoblastoma in colorectal cancer |
| title_full | MiR-26a downregulates retinoblastoma in colorectal cancer |
| title_fullStr | MiR-26a downregulates retinoblastoma in colorectal cancer |
| title_full_unstemmed | MiR-26a downregulates retinoblastoma in colorectal cancer |
| title_short | MiR-26a downregulates retinoblastoma in colorectal cancer |
| title_sort | mir 26a downregulates retinoblastoma in colorectal cancer |
| url | https://doi.org/10.1177/1010428317695945 |
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