Construction and Application of Au NRs/4-MBA/PAM Ratiometric Surface-Enhanced Raman Scattering Substrate for Fish Veterinary Drug Residue Detection
Surface-enhanced Raman scattering (SERS) is widely used for trace detection of substances, and the key to this technology lies in the preparation of the substrate material. In this study, a composite SERS material of Au NRs/4-MBA/PAM was constructed and characterized to better immobilize the referen...
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MDPI AG
2024-11-01
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| author | Jianxing Yu Huiping Fu Qing Gu |
| author_facet | Jianxing Yu Huiping Fu Qing Gu |
| author_sort | Jianxing Yu |
| collection | DOAJ |
| description | Surface-enhanced Raman scattering (SERS) is widely used for trace detection of substances, and the key to this technology lies in the preparation of the substrate material. In this study, a composite SERS material of Au NRs/4-MBA/PAM was constructed and characterized to better immobilize the reference molecule 4-mercaptobenzoic acid (4-MBA). Electron transmission microscopy results demonstrated that the PAM film helps Au NRs to pack closely, enhancing the stability of the material structure and reducing the interference of external environmental factors on the response of 4-MBA, thus improving the accuracy of quantitative determination. Comparative experimental results with the Au NRs/4-MBA substrate showed that the relative standard deviations (RSDs) of the detection results for MG on different batches of Au NRs/4-MBA/PAM were less than 8.0%, and the RSDs of different points on the same material were less than 10.0%, indicating that the Au NRs/4-MBA/PAM has higher uniformity, better reproducibility, and higher sensitivity in detecting malachite green (MG). Applying this material in the recovery determination of fish extract showed that the recovery rates of MG were between 75.60% and 83.24%. Therefore, the Au NRs/4-MBA/PAM substrate can accurately detect and quantify veterinary drug residue in complex matrices such as food tissue. |
| format | Article |
| id | doaj-art-98d69c7f6d3345d1ab401f8f174d7ff6 |
| institution | DOAJ |
| issn | 2079-4991 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Nanomaterials |
| spelling | doaj-art-98d69c7f6d3345d1ab401f8f174d7ff62025-08-20T02:48:06ZengMDPI AGNanomaterials2079-49912024-11-011422177410.3390/nano14221774Construction and Application of Au NRs/4-MBA/PAM Ratiometric Surface-Enhanced Raman Scattering Substrate for Fish Veterinary Drug Residue DetectionJianxing Yu0Huiping Fu1Qing Gu2Zhejiang Key Laboratory of Food Microbiology and Nutritional Health, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaCollege of Chemistry, Fuzhou University, Fuzhou 350116, ChinaZhejiang Key Laboratory of Food Microbiology and Nutritional Health, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaSurface-enhanced Raman scattering (SERS) is widely used for trace detection of substances, and the key to this technology lies in the preparation of the substrate material. In this study, a composite SERS material of Au NRs/4-MBA/PAM was constructed and characterized to better immobilize the reference molecule 4-mercaptobenzoic acid (4-MBA). Electron transmission microscopy results demonstrated that the PAM film helps Au NRs to pack closely, enhancing the stability of the material structure and reducing the interference of external environmental factors on the response of 4-MBA, thus improving the accuracy of quantitative determination. Comparative experimental results with the Au NRs/4-MBA substrate showed that the relative standard deviations (RSDs) of the detection results for MG on different batches of Au NRs/4-MBA/PAM were less than 8.0%, and the RSDs of different points on the same material were less than 10.0%, indicating that the Au NRs/4-MBA/PAM has higher uniformity, better reproducibility, and higher sensitivity in detecting malachite green (MG). Applying this material in the recovery determination of fish extract showed that the recovery rates of MG were between 75.60% and 83.24%. Therefore, the Au NRs/4-MBA/PAM substrate can accurately detect and quantify veterinary drug residue in complex matrices such as food tissue.https://www.mdpi.com/2079-4991/14/22/1774Au nanorodssurface-enhanced Raman scatteringpolyacrylamide4-mercaptobenzoic acidveterinary drug residues |
| spellingShingle | Jianxing Yu Huiping Fu Qing Gu Construction and Application of Au NRs/4-MBA/PAM Ratiometric Surface-Enhanced Raman Scattering Substrate for Fish Veterinary Drug Residue Detection Nanomaterials Au nanorods surface-enhanced Raman scattering polyacrylamide 4-mercaptobenzoic acid veterinary drug residues |
| title | Construction and Application of Au NRs/4-MBA/PAM Ratiometric Surface-Enhanced Raman Scattering Substrate for Fish Veterinary Drug Residue Detection |
| title_full | Construction and Application of Au NRs/4-MBA/PAM Ratiometric Surface-Enhanced Raman Scattering Substrate for Fish Veterinary Drug Residue Detection |
| title_fullStr | Construction and Application of Au NRs/4-MBA/PAM Ratiometric Surface-Enhanced Raman Scattering Substrate for Fish Veterinary Drug Residue Detection |
| title_full_unstemmed | Construction and Application of Au NRs/4-MBA/PAM Ratiometric Surface-Enhanced Raman Scattering Substrate for Fish Veterinary Drug Residue Detection |
| title_short | Construction and Application of Au NRs/4-MBA/PAM Ratiometric Surface-Enhanced Raman Scattering Substrate for Fish Veterinary Drug Residue Detection |
| title_sort | construction and application of au nrs 4 mba pam ratiometric surface enhanced raman scattering substrate for fish veterinary drug residue detection |
| topic | Au nanorods surface-enhanced Raman scattering polyacrylamide 4-mercaptobenzoic acid veterinary drug residues |
| url | https://www.mdpi.com/2079-4991/14/22/1774 |
| work_keys_str_mv | AT jianxingyu constructionandapplicationofaunrs4mbapamratiometricsurfaceenhancedramanscatteringsubstrateforfishveterinarydrugresiduedetection AT huipingfu constructionandapplicationofaunrs4mbapamratiometricsurfaceenhancedramanscatteringsubstrateforfishveterinarydrugresiduedetection AT qinggu constructionandapplicationofaunrs4mbapamratiometricsurfaceenhancedramanscatteringsubstrateforfishveterinarydrugresiduedetection |