Effect of sodium hypochlorite and EDTA pretreatment on the resin infiltration efficacy and acid resistance of enamel white spot lesions: an in vitro study
Abstract Background This in vitro study aimed to evaluate the effect of sodium hypochlorite (NaOCl) and ethylene diamine tetraacetic acid (EDTA) as pretreatments on the resin infiltration efficacy and acid resistance of enamel white spot lesions (WSLs). Methods Enamel blocks prepared from the buccal...
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2024-11-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-024-05238-x |
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| author | Ke Ni Lijuan Chen Jingyu He Yu Ding Jian Meng Qingfei Meng Karl Lyons May Lei Mei |
| author_facet | Ke Ni Lijuan Chen Jingyu He Yu Ding Jian Meng Qingfei Meng Karl Lyons May Lei Mei |
| author_sort | Ke Ni |
| collection | DOAJ |
| description | Abstract Background This in vitro study aimed to evaluate the effect of sodium hypochlorite (NaOCl) and ethylene diamine tetraacetic acid (EDTA) as pretreatments on the resin infiltration efficacy and acid resistance of enamel white spot lesions (WSLs). Methods Enamel blocks prepared from the buccal surfaces of sound human premolars were placed in a demineralisation solution for 3 days to establish artificial enamel WSLs. All the blocks with WSLs were randomly divided into four groups (n = 40 per group): RI (Control): resin infiltration only. N/RI: 5.25% NaOCl pretreatment + resin infiltration; E/RI: 17% EDTA pretreatment + resin infiltration; NE/RI: combined pretreatment of 5.25% NaOCl with 17% EDTA + resin infiltration; Enamel pore exposure was measured by using SEM observation and Image J software in each group after pretreatment prior to resin infiltration (n = 8 per group). Resin penetration depth and enamel surface microhardness were assessed after resin infiltration (n = 8 per group for each test) by a confocal laser scanning microscope and a Vickers’ microhardness test respectively. The remaining specimens were subjected 7 days of pH cycling after which surface enamel roughness and microhardness were assessed by an atomic force microscope and a microhardness test respectively. Results Compared to the control group, the enamel surface pore exposure (p = 0.007), resin penetration depth (p = 0.011) and enamel surface microhardness (p < 0.001) were all significantly increased after the pretreatment of 5.25% NaOCl alone or combined with 17% EDTA by one-way ANOVA. After pH cycling, no significant differences were found in enamel surface roughness or microhardness among the four groups (p = 0.55) by two-way ANOVA. Conclusions Enamel surface pretreatment of 5.25% NaOCl or combined with 17% EDTA improved the efficacy of the resin infiltration of WSLs, but might be useless in enhancing the acid resistance of resin-infiltrated WSLs. |
| format | Article |
| id | doaj-art-98d30728396e4a39a0974e9545e48144 |
| institution | DOAJ |
| issn | 1472-6831 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | BMC Oral Health |
| spelling | doaj-art-98d30728396e4a39a0974e9545e481442025-08-20T02:49:09ZengBMCBMC Oral Health1472-68312024-11-0124111210.1186/s12903-024-05238-xEffect of sodium hypochlorite and EDTA pretreatment on the resin infiltration efficacy and acid resistance of enamel white spot lesions: an in vitro studyKe Ni0Lijuan Chen1Jingyu He2Yu Ding3Jian Meng4Qingfei Meng5Karl Lyons6May Lei Mei7Department of Stomatology, Xuzhou Clinical School of Xuzhou Medical UniversityDepartment of Stomatology, The Affiliated Xuzhou Municipal Hospital of Xuzhou Medical UniversityDepartment of Stomatology, Xuzhou Clinical School of Xuzhou Medical UniversityDepartment of Stomatology, Xuzhou Clinical School of Xuzhou Medical UniversityDepartment of Stomatology, Xuzhou Clinical School of Xuzhou Medical UniversityDepartment of Stomatology, Xuzhou Clinical School of Xuzhou Medical UniversityFaculty of Dentistry, University of OtagoFaculty of Dentistry, University of OtagoAbstract Background This in vitro study aimed to evaluate the effect of sodium hypochlorite (NaOCl) and ethylene diamine tetraacetic acid (EDTA) as pretreatments on the resin infiltration efficacy and acid resistance of enamel white spot lesions (WSLs). Methods Enamel blocks prepared from the buccal surfaces of sound human premolars were placed in a demineralisation solution for 3 days to establish artificial enamel WSLs. All the blocks with WSLs were randomly divided into four groups (n = 40 per group): RI (Control): resin infiltration only. N/RI: 5.25% NaOCl pretreatment + resin infiltration; E/RI: 17% EDTA pretreatment + resin infiltration; NE/RI: combined pretreatment of 5.25% NaOCl with 17% EDTA + resin infiltration; Enamel pore exposure was measured by using SEM observation and Image J software in each group after pretreatment prior to resin infiltration (n = 8 per group). Resin penetration depth and enamel surface microhardness were assessed after resin infiltration (n = 8 per group for each test) by a confocal laser scanning microscope and a Vickers’ microhardness test respectively. The remaining specimens were subjected 7 days of pH cycling after which surface enamel roughness and microhardness were assessed by an atomic force microscope and a microhardness test respectively. Results Compared to the control group, the enamel surface pore exposure (p = 0.007), resin penetration depth (p = 0.011) and enamel surface microhardness (p < 0.001) were all significantly increased after the pretreatment of 5.25% NaOCl alone or combined with 17% EDTA by one-way ANOVA. After pH cycling, no significant differences were found in enamel surface roughness or microhardness among the four groups (p = 0.55) by two-way ANOVA. Conclusions Enamel surface pretreatment of 5.25% NaOCl or combined with 17% EDTA improved the efficacy of the resin infiltration of WSLs, but might be useless in enhancing the acid resistance of resin-infiltrated WSLs.https://doi.org/10.1186/s12903-024-05238-xWhite spot lesionsResin infiltrationPretreatmentNaOClEDTA |
| spellingShingle | Ke Ni Lijuan Chen Jingyu He Yu Ding Jian Meng Qingfei Meng Karl Lyons May Lei Mei Effect of sodium hypochlorite and EDTA pretreatment on the resin infiltration efficacy and acid resistance of enamel white spot lesions: an in vitro study BMC Oral Health White spot lesions Resin infiltration Pretreatment NaOCl EDTA |
| title | Effect of sodium hypochlorite and EDTA pretreatment on the resin infiltration efficacy and acid resistance of enamel white spot lesions: an in vitro study |
| title_full | Effect of sodium hypochlorite and EDTA pretreatment on the resin infiltration efficacy and acid resistance of enamel white spot lesions: an in vitro study |
| title_fullStr | Effect of sodium hypochlorite and EDTA pretreatment on the resin infiltration efficacy and acid resistance of enamel white spot lesions: an in vitro study |
| title_full_unstemmed | Effect of sodium hypochlorite and EDTA pretreatment on the resin infiltration efficacy and acid resistance of enamel white spot lesions: an in vitro study |
| title_short | Effect of sodium hypochlorite and EDTA pretreatment on the resin infiltration efficacy and acid resistance of enamel white spot lesions: an in vitro study |
| title_sort | effect of sodium hypochlorite and edta pretreatment on the resin infiltration efficacy and acid resistance of enamel white spot lesions an in vitro study |
| topic | White spot lesions Resin infiltration Pretreatment NaOCl EDTA |
| url | https://doi.org/10.1186/s12903-024-05238-x |
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