Isolation and characterization of a highly pathogenic strain of Vibrio harveyi EFL-2201 from pearl gentian grouper
In May 2022, a significant mortality event involving pearl gentian groupers (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) was reported at an aquaculture farm in Zhangzhou, China, under water conditions of approximately 25 °C, pH 8.0, and salinity 32 ‰. A highly pathogenic bacterial strain, EFL-2201...
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| Language: | English |
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Elsevier
2025-04-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000547 |
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| author | Biao Yuan Zhixia Zhuang Xiaoru Wang Huabin Huang Qingpi Yan |
| author_facet | Biao Yuan Zhixia Zhuang Xiaoru Wang Huabin Huang Qingpi Yan |
| author_sort | Biao Yuan |
| collection | DOAJ |
| description | In May 2022, a significant mortality event involving pearl gentian groupers (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) was reported at an aquaculture farm in Zhangzhou, China, under water conditions of approximately 25 °C, pH 8.0, and salinity 32 ‰. A highly pathogenic bacterial strain, EFL-2201, was isolated from the spleen of a diseased fish. The outbreak was traced back to Vibrio harveyi through a series of scientific methods including isolation, identification, and regression infection. In vitro assays indicated that the conditions during the outbreak were conducive to the proliferation, motility, biofilm formation, and chemotaxis of EFL-2201. Whole-genome sequencing of the strain revealed that mechanisms related to adhesion, secretion, toxins, and invasion significantly contributed to its pathogenicity. Temporal and spatial bacterial load of EFL-2201 in various tissues of the infected groupers indicated that bacterial loads peaked at 24 h post-infection (hpi). The spleen, which had the highest bacterial load among all tissues, was subjected to transcriptome sequencing at 24 hpi, revealing significant enrichment in pathways related to immunity, cell cycle, and red blood cell oxygen transport. In conclusion, this study unveiled the pathogenic mechanisms of V. harveyi and immune response mechanisms of pearl gentian grouper spleens to infection, laying a theoretical foundation for the prevention and control of V. harveyi-induced disease. |
| format | Article |
| id | doaj-art-98cd6e198eca42b3bc72d8a3f3bd1b91 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-98cd6e198eca42b3bc72d8a3f3bd1b912025-02-12T05:31:18ZengElsevierAquaculture Reports2352-51342025-04-0141102668Isolation and characterization of a highly pathogenic strain of Vibrio harveyi EFL-2201 from pearl gentian grouperBiao Yuan0Zhixia Zhuang1Xiaoru Wang2Huabin Huang3Qingpi Yan4College of Environment and Public Health, Xiamen Huaxia University, Xiamen, Fujian 361024, ChinaCollege of Environment and Public Health, Xiamen Huaxia University, Xiamen, Fujian 361024, ChinaCollege of Environment and Public Health, Xiamen Huaxia University, Xiamen, Fujian 361024, ChinaCollege of Environment and Public Health, Xiamen Huaxia University, Xiamen, Fujian 361024, ChinaFisheries College, Jimei University, Xiamen, Fujian 361021, China; Corresponding author.In May 2022, a significant mortality event involving pearl gentian groupers (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) was reported at an aquaculture farm in Zhangzhou, China, under water conditions of approximately 25 °C, pH 8.0, and salinity 32 ‰. A highly pathogenic bacterial strain, EFL-2201, was isolated from the spleen of a diseased fish. The outbreak was traced back to Vibrio harveyi through a series of scientific methods including isolation, identification, and regression infection. In vitro assays indicated that the conditions during the outbreak were conducive to the proliferation, motility, biofilm formation, and chemotaxis of EFL-2201. Whole-genome sequencing of the strain revealed that mechanisms related to adhesion, secretion, toxins, and invasion significantly contributed to its pathogenicity. Temporal and spatial bacterial load of EFL-2201 in various tissues of the infected groupers indicated that bacterial loads peaked at 24 h post-infection (hpi). The spleen, which had the highest bacterial load among all tissues, was subjected to transcriptome sequencing at 24 hpi, revealing significant enrichment in pathways related to immunity, cell cycle, and red blood cell oxygen transport. In conclusion, this study unveiled the pathogenic mechanisms of V. harveyi and immune response mechanisms of pearl gentian grouper spleens to infection, laying a theoretical foundation for the prevention and control of V. harveyi-induced disease.http://www.sciencedirect.com/science/article/pii/S2352513425000547Vibrio harveyiPearl gentian grouperPathogenTranscriptome sequencingVirulence |
| spellingShingle | Biao Yuan Zhixia Zhuang Xiaoru Wang Huabin Huang Qingpi Yan Isolation and characterization of a highly pathogenic strain of Vibrio harveyi EFL-2201 from pearl gentian grouper Aquaculture Reports Vibrio harveyi Pearl gentian grouper Pathogen Transcriptome sequencing Virulence |
| title | Isolation and characterization of a highly pathogenic strain of Vibrio harveyi EFL-2201 from pearl gentian grouper |
| title_full | Isolation and characterization of a highly pathogenic strain of Vibrio harveyi EFL-2201 from pearl gentian grouper |
| title_fullStr | Isolation and characterization of a highly pathogenic strain of Vibrio harveyi EFL-2201 from pearl gentian grouper |
| title_full_unstemmed | Isolation and characterization of a highly pathogenic strain of Vibrio harveyi EFL-2201 from pearl gentian grouper |
| title_short | Isolation and characterization of a highly pathogenic strain of Vibrio harveyi EFL-2201 from pearl gentian grouper |
| title_sort | isolation and characterization of a highly pathogenic strain of vibrio harveyi efl 2201 from pearl gentian grouper |
| topic | Vibrio harveyi Pearl gentian grouper Pathogen Transcriptome sequencing Virulence |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000547 |
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