Development and validation of rapid eDNA detection method for yellow mud turtle, Kinosternon flavescens: a field study in South Texas, USA
The conservation of freshwater turtle species depends on precise and effective monitoring techniques. Environmental DNA (eDNA) analysis is a potential method for identifying cryptic and elusive turtle species in aquatic ecosystems. eDNA analysis can help to identify key regions for conservation effo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Conservation Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcosc.2025.1431348/full |
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| author | Sakib Tahmid Rishan Richard J. Kline Richard J. Kline Md Saydur Rahman Md Saydur Rahman |
| author_facet | Sakib Tahmid Rishan Richard J. Kline Richard J. Kline Md Saydur Rahman Md Saydur Rahman |
| author_sort | Sakib Tahmid Rishan |
| collection | DOAJ |
| description | The conservation of freshwater turtle species depends on precise and effective monitoring techniques. Environmental DNA (eDNA) analysis is a potential method for identifying cryptic and elusive turtle species in aquatic ecosystems. eDNA analysis can help to identify key regions for conservation efforts and monitor changes in population levels over time. This study aims to evaluate the effectiveness of a rapid eDNA detection method for the yellow mud turtle (Kinosternon flavescens, an indicator species that is endangered in some states in the USA), which inhabits local oxbow lakes (e.g., resacas) in Cameron County, South Texas. A species-specific nested PCR assay was designed to enhance the detection of yellow mud turtle species. Water samples were collected from five locations within Cameron County for the detection of yellow mud turtle eDNA. Our results revealed the presence of yellow mud turtles in two out of the five surveyed locations. Our study shows great potential for eDNA monitoring for yellow mud turtle species. This study also provides insights on using eDNA monitoring to protect yellow mud turtle species and recommendations for future research and conservation initiatives. |
| format | Article |
| id | doaj-art-98c6120cb2b543178420047365d5ca33 |
| institution | Kabale University |
| issn | 2673-611X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Conservation Science |
| spelling | doaj-art-98c6120cb2b543178420047365d5ca332025-01-24T07:13:32ZengFrontiers Media S.A.Frontiers in Conservation Science2673-611X2025-01-01610.3389/fcosc.2025.14313481431348Development and validation of rapid eDNA detection method for yellow mud turtle, Kinosternon flavescens: a field study in South Texas, USASakib Tahmid Rishan0Richard J. Kline1Richard J. Kline2Md Saydur Rahman3Md Saydur Rahman4Biochemistry and Molecular Biology Program, School of Integrative Biological and Chemical Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesBiochemistry and Molecular Biology Program, School of Integrative Biological and Chemical Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesSchool of Earth, Environmental, and Marine Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesBiochemistry and Molecular Biology Program, School of Integrative Biological and Chemical Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesSchool of Earth, Environmental, and Marine Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesThe conservation of freshwater turtle species depends on precise and effective monitoring techniques. Environmental DNA (eDNA) analysis is a potential method for identifying cryptic and elusive turtle species in aquatic ecosystems. eDNA analysis can help to identify key regions for conservation efforts and monitor changes in population levels over time. This study aims to evaluate the effectiveness of a rapid eDNA detection method for the yellow mud turtle (Kinosternon flavescens, an indicator species that is endangered in some states in the USA), which inhabits local oxbow lakes (e.g., resacas) in Cameron County, South Texas. A species-specific nested PCR assay was designed to enhance the detection of yellow mud turtle species. Water samples were collected from five locations within Cameron County for the detection of yellow mud turtle eDNA. Our results revealed the presence of yellow mud turtles in two out of the five surveyed locations. Our study shows great potential for eDNA monitoring for yellow mud turtle species. This study also provides insights on using eDNA monitoring to protect yellow mud turtle species and recommendations for future research and conservation initiatives.https://www.frontiersin.org/articles/10.3389/fcosc.2025.1431348/fullenvironmental DNAbiodiversity monitoringnature conservationglobal ecologymud turtles |
| spellingShingle | Sakib Tahmid Rishan Richard J. Kline Richard J. Kline Md Saydur Rahman Md Saydur Rahman Development and validation of rapid eDNA detection method for yellow mud turtle, Kinosternon flavescens: a field study in South Texas, USA Frontiers in Conservation Science environmental DNA biodiversity monitoring nature conservation global ecology mud turtles |
| title | Development and validation of rapid eDNA detection method for yellow mud turtle, Kinosternon flavescens: a field study in South Texas, USA |
| title_full | Development and validation of rapid eDNA detection method for yellow mud turtle, Kinosternon flavescens: a field study in South Texas, USA |
| title_fullStr | Development and validation of rapid eDNA detection method for yellow mud turtle, Kinosternon flavescens: a field study in South Texas, USA |
| title_full_unstemmed | Development and validation of rapid eDNA detection method for yellow mud turtle, Kinosternon flavescens: a field study in South Texas, USA |
| title_short | Development and validation of rapid eDNA detection method for yellow mud turtle, Kinosternon flavescens: a field study in South Texas, USA |
| title_sort | development and validation of rapid edna detection method for yellow mud turtle kinosternon flavescens a field study in south texas usa |
| topic | environmental DNA biodiversity monitoring nature conservation global ecology mud turtles |
| url | https://www.frontiersin.org/articles/10.3389/fcosc.2025.1431348/full |
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