A Novel, Cell-Compatible Hyaluronidase Activity Assay Identifies Dextran Sulfates and Other Sulfated Polymeric Hydrocarbons as Potent Inhibitors for CEMIP
Hyaluronan (HA) levels are dynamically regulated homeostatically through biosynthesis and degradation. HA homeostasis is often perturbed under disease conditions. HA degradation products are thought to contribute to disease pathology. The hyaluronidase CEMIP requires the presence of living cells for...
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2025-01-01
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| author | Anja Schmaus Sofia Spataro Paul Sallmann Stephanie Möller Leonardo Scapozza Marco Prunotto Jonathan P. Sleeman |
| author_facet | Anja Schmaus Sofia Spataro Paul Sallmann Stephanie Möller Leonardo Scapozza Marco Prunotto Jonathan P. Sleeman |
| author_sort | Anja Schmaus |
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| description | Hyaluronan (HA) levels are dynamically regulated homeostatically through biosynthesis and degradation. HA homeostasis is often perturbed under disease conditions. HA degradation products are thought to contribute to disease pathology. The hyaluronidase CEMIP requires the presence of living cells for its HA depolymerizing activity. CEMIP is overexpressed in a variety of pathological conditions, and the inhibition of its hyaluronidase activity therefore has therapeutic potential. To identify novel inhibitors of the CEMIP hyaluronidase activity, we established here a cell-compatible, medium-throughput assay for CEMIP-dependent HA depolymerization. The assay employs ultrafiltration plates to separate low- from high-molecular-weight HA, followed by quantification of HA fragments using an HA ELISA-like assay. Using this assay, we tested a range of compounds that have been reported to inhibit other hyaluronidases. Thereby, we identified several sulfated hydrocarbon polymers that inhibit CEMIP more potently than other hyaluronidases. One of these is heparin, a sulfated glycosaminoglycan produced by mast cells that constitutes the first described physiological CEMIP inhibitor. The most potent inhibitor (IC<sub>50</sub> of 1.8 nM) is dextran sulfate, a synthetic sulfated polysaccharide. Heparin and dextran sulfate are used in numerous established and experimental biomedical applications. Their ability to inhibit CEMIP needs to be taken into account in these contexts. |
| format | Article |
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| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-98b745bf272842babcd88a5ec39b47812025-01-24T13:26:41ZengMDPI AGCells2073-44092025-01-0114210110.3390/cells14020101A Novel, Cell-Compatible Hyaluronidase Activity Assay Identifies Dextran Sulfates and Other Sulfated Polymeric Hydrocarbons as Potent Inhibitors for CEMIPAnja Schmaus0Sofia Spataro1Paul Sallmann2Stephanie Möller3Leonardo Scapozza4Marco Prunotto5Jonathan P. Sleeman6European Center for Angioscience (ECAS), Medical Faculty Mannheim, University of Heidelberg, 68167 Mannheim, GermanySchool of Pharmaceutical Sciences, University of Geneva, Rue Michel-Servet 1, 1211 Geneva, SwitzerlandEuropean Center for Angioscience (ECAS), Medical Faculty Mannheim, University of Heidelberg, 68167 Mannheim, GermanyBiomaterials Department, INNOVENT e.V., Prüssingstrasse 27b, 07745 Jena, GermanySchool of Pharmaceutical Sciences, University of Geneva, Rue Michel-Servet 1, 1211 Geneva, SwitzerlandSchool of Pharmaceutical Sciences, University of Geneva, Rue Michel-Servet 1, 1211 Geneva, SwitzerlandEuropean Center for Angioscience (ECAS), Medical Faculty Mannheim, University of Heidelberg, 68167 Mannheim, GermanyHyaluronan (HA) levels are dynamically regulated homeostatically through biosynthesis and degradation. HA homeostasis is often perturbed under disease conditions. HA degradation products are thought to contribute to disease pathology. The hyaluronidase CEMIP requires the presence of living cells for its HA depolymerizing activity. CEMIP is overexpressed in a variety of pathological conditions, and the inhibition of its hyaluronidase activity therefore has therapeutic potential. To identify novel inhibitors of the CEMIP hyaluronidase activity, we established here a cell-compatible, medium-throughput assay for CEMIP-dependent HA depolymerization. The assay employs ultrafiltration plates to separate low- from high-molecular-weight HA, followed by quantification of HA fragments using an HA ELISA-like assay. Using this assay, we tested a range of compounds that have been reported to inhibit other hyaluronidases. Thereby, we identified several sulfated hydrocarbon polymers that inhibit CEMIP more potently than other hyaluronidases. One of these is heparin, a sulfated glycosaminoglycan produced by mast cells that constitutes the first described physiological CEMIP inhibitor. The most potent inhibitor (IC<sub>50</sub> of 1.8 nM) is dextran sulfate, a synthetic sulfated polysaccharide. Heparin and dextran sulfate are used in numerous established and experimental biomedical applications. Their ability to inhibit CEMIP needs to be taken into account in these contexts.https://www.mdpi.com/2073-4409/14/2/101CEMIPHYBIDhyaluronidasehyaluronandextran sulfateheparin |
| spellingShingle | Anja Schmaus Sofia Spataro Paul Sallmann Stephanie Möller Leonardo Scapozza Marco Prunotto Jonathan P. Sleeman A Novel, Cell-Compatible Hyaluronidase Activity Assay Identifies Dextran Sulfates and Other Sulfated Polymeric Hydrocarbons as Potent Inhibitors for CEMIP Cells CEMIP HYBID hyaluronidase hyaluronan dextran sulfate heparin |
| title | A Novel, Cell-Compatible Hyaluronidase Activity Assay Identifies Dextran Sulfates and Other Sulfated Polymeric Hydrocarbons as Potent Inhibitors for CEMIP |
| title_full | A Novel, Cell-Compatible Hyaluronidase Activity Assay Identifies Dextran Sulfates and Other Sulfated Polymeric Hydrocarbons as Potent Inhibitors for CEMIP |
| title_fullStr | A Novel, Cell-Compatible Hyaluronidase Activity Assay Identifies Dextran Sulfates and Other Sulfated Polymeric Hydrocarbons as Potent Inhibitors for CEMIP |
| title_full_unstemmed | A Novel, Cell-Compatible Hyaluronidase Activity Assay Identifies Dextran Sulfates and Other Sulfated Polymeric Hydrocarbons as Potent Inhibitors for CEMIP |
| title_short | A Novel, Cell-Compatible Hyaluronidase Activity Assay Identifies Dextran Sulfates and Other Sulfated Polymeric Hydrocarbons as Potent Inhibitors for CEMIP |
| title_sort | novel cell compatible hyaluronidase activity assay identifies dextran sulfates and other sulfated polymeric hydrocarbons as potent inhibitors for cemip |
| topic | CEMIP HYBID hyaluronidase hyaluronan dextran sulfate heparin |
| url | https://www.mdpi.com/2073-4409/14/2/101 |
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