Construction and optimization of a nisin-controlled expression vector using a pre-screened strong promoter in Streptococcus thermophilus

The nisin-controlled gene expression (NICE) system is an efficient and promising gene expression system for Lactococcus lactis. To enhance the expression efficiency of the NICE system in Streptococcus thermophilus ATCC19258, an inducible expression vector, pNZ8148-PnisA-gfp-PnisR-nisR-nisK, containi...

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Bibliographic Details
Main Authors: Yanxin Ye, Ruiting Zhao, Leilei Li, Zhi Li, Yanyan Chen, Zhenshang Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1586348/full
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