Clinical, epidemiological, and pathological findings of ovine gammaherpesvirus 2 infections in cattle from Southern Brazil
Introduction: Sheep associated-malignant catarrhal fever is a frequently fatal, lymphoproliferative, and vascular disease caused by ovine gammaherpesvirus 2 (OvGHV2), a member of the malignant catarrhal fever virus (MCFV) complex. OvGHV2-related epidemics normally have reduced morbidity with elevat...
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The Journal of Infection in Developing Countries
2025-01-01
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| author | Selwyn Arlington Headley Juliana Torres Tomazi Fritzen Érika Fernandes Lopes Maturana Natali Araujo Correia Alves de Alvarenga Rafael Vince Rodrigues Ana Laura Paulino Leite Gomes Maria Constanza Rodriguez Maria Izabel Pedra Sogari Ana Aparecida Correa Xavier Flávia Helena Pereira Silva Priscilla Fajardo Valente Pereira Vitor Bruno Bianconi Rosa Karina Keller Marques da Costa Flaiban Giovana Wingeter Di Santis Thais Helena Constantino Patelli Júlio Augusto Naylor Lisbôa Amauri Alcindo Alfieri |
| author_facet | Selwyn Arlington Headley Juliana Torres Tomazi Fritzen Érika Fernandes Lopes Maturana Natali Araujo Correia Alves de Alvarenga Rafael Vince Rodrigues Ana Laura Paulino Leite Gomes Maria Constanza Rodriguez Maria Izabel Pedra Sogari Ana Aparecida Correa Xavier Flávia Helena Pereira Silva Priscilla Fajardo Valente Pereira Vitor Bruno Bianconi Rosa Karina Keller Marques da Costa Flaiban Giovana Wingeter Di Santis Thais Helena Constantino Patelli Júlio Augusto Naylor Lisbôa Amauri Alcindo Alfieri |
| author_sort | Selwyn Arlington Headley |
| collection | DOAJ |
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Introduction: Sheep associated-malignant catarrhal fever is a frequently fatal, lymphoproliferative, and vascular disease caused by ovine gammaherpesvirus 2 (OvGHV2), a member of the malignant catarrhal fever virus (MCFV) complex. OvGHV2-related epidemics normally have reduced morbidity with elevated lethality.
Methodology: This study investigated the causes of elevated morbidity, mortality, and lethality in cattle maintained on Brachiaria grass pastures and with neurological and enteric disease syndromes from nine farms in Paraná, Southern Brazil.
Results: The principal histopathological findings included necrotizing lymphocytic vasculitis, proliferative vascular lesions, and toxic cholangiohepatitis. An immunohistochemical (IHC) assay utilizing the 15A monoclonal antibody (15A-MAb), which is specific for MCFV, revealed positive intracytoplasmic immunoreactivity within the epithelial cells of the lungs, intestine, liver, and kidneys in most animals, confirming infections by MCFV. PCR detected singular infections by OvGHV2 (n = 3) and bovine gammaherpesvirus 6 BoGHV6 (n = 3) in cattle with positive intracytoplasmic immunoreactivity by the 15A-MAb IHC assay. In one animal with positive immunoreactivity to the 15A-MAb IHC assay, neither Macavirus was identified through molecular testing.
Conclusions: These findings suggested that these two Macavirus were associated with the positive IHC findings. Additionally, the non-detection of OvGHV2 and BoGHV6 in the organs of one animal, despite typical vascular lesions and with positive IHC results, suggests that another Macavirus may be involved. Moreover, the identification of BoGHV6 DNA in cattle not infected by OvGHV2 but containing MCFV antigens, indicates cross-reactivity of BoGHV6 with the 15A-MAb assay. The possibe role of Bracharia on the occurrence of these infections is discussed.
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| format | Article |
| id | doaj-art-98ab80ee879a49e2bf1470d4a78e43ad |
| institution | Kabale University |
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| publishDate | 2025-01-01 |
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| spelling | doaj-art-98ab80ee879a49e2bf1470d4a78e43ad2025-08-20T03:52:43ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802025-01-01190110.3855/jidc.19951Clinical, epidemiological, and pathological findings of ovine gammaherpesvirus 2 infections in cattle from Southern BrazilSelwyn Arlington Headley0Juliana Torres Tomazi Fritzen1Érika Fernandes Lopes Maturana2Natali Araujo Correia Alves de Alvarenga3Rafael Vince Rodrigues4Ana Laura Paulino Leite Gomes5Maria Constanza Rodriguez6Maria Izabel Pedra Sogari7Ana Aparecida Correa Xavier8Flávia Helena Pereira Silva9Priscilla Fajardo Valente Pereira10Vitor Bruno Bianconi Rosa11Karina Keller Marques da Costa Flaiban12Giovana Wingeter Di Santis13Thais Helena Constantino Patelli14Júlio Augusto Naylor Lisbôa15Amauri Alcindo Alfieri16Laboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilLaboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilLarge Animal Internal Medicine, Department of Veterinary Clinics, Universidade Estadual de Londrina, Londrina, Paraná, BrazilLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilMarcos Enrietti Diagnostic Center, Agência de Defesa Agropecuária do Paraná (ADAPAR), Curitiba, Paraná, BrazilLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilLarge Animal Internal Medicine, Department of Veterinary Clinics, Universidade Estadual de Londrina, Londrina, Paraná, BrazilDepartment of Veterinary Medicine and Animal Production, Universidade Estadual do Norte do Paraná, Bandeirantes, BrazilLaboratory of Clinical Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, BrazilDepartment of Veterinary Medicine and Animal Production, Universidade Estadual do Norte do Paraná, Bandeirantes, BrazilLarge Animal Internal Medicine, Department of Veterinary Clinics, Universidade Estadual de Londrina, Londrina, Paraná, BrazilMulti-User Animal Health Laboratory (LAMSA), Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil Introduction: Sheep associated-malignant catarrhal fever is a frequently fatal, lymphoproliferative, and vascular disease caused by ovine gammaherpesvirus 2 (OvGHV2), a member of the malignant catarrhal fever virus (MCFV) complex. OvGHV2-related epidemics normally have reduced morbidity with elevated lethality. Methodology: This study investigated the causes of elevated morbidity, mortality, and lethality in cattle maintained on Brachiaria grass pastures and with neurological and enteric disease syndromes from nine farms in Paraná, Southern Brazil. Results: The principal histopathological findings included necrotizing lymphocytic vasculitis, proliferative vascular lesions, and toxic cholangiohepatitis. An immunohistochemical (IHC) assay utilizing the 15A monoclonal antibody (15A-MAb), which is specific for MCFV, revealed positive intracytoplasmic immunoreactivity within the epithelial cells of the lungs, intestine, liver, and kidneys in most animals, confirming infections by MCFV. PCR detected singular infections by OvGHV2 (n = 3) and bovine gammaherpesvirus 6 BoGHV6 (n = 3) in cattle with positive intracytoplasmic immunoreactivity by the 15A-MAb IHC assay. In one animal with positive immunoreactivity to the 15A-MAb IHC assay, neither Macavirus was identified through molecular testing. Conclusions: These findings suggested that these two Macavirus were associated with the positive IHC findings. Additionally, the non-detection of OvGHV2 and BoGHV6 in the organs of one animal, despite typical vascular lesions and with positive IHC results, suggests that another Macavirus may be involved. Moreover, the identification of BoGHV6 DNA in cattle not infected by OvGHV2 but containing MCFV antigens, indicates cross-reactivity of BoGHV6 with the 15A-MAb assay. The possibe role of Bracharia on the occurrence of these infections is discussed. https://jidc.org/index.php/journal/article/view/19951Bovine gammaherpesvirus 6Brachiaria spppoisoningdiagnostic immunohistochemistryMacavirusmalignant catarrhal fever |
| spellingShingle | Selwyn Arlington Headley Juliana Torres Tomazi Fritzen Érika Fernandes Lopes Maturana Natali Araujo Correia Alves de Alvarenga Rafael Vince Rodrigues Ana Laura Paulino Leite Gomes Maria Constanza Rodriguez Maria Izabel Pedra Sogari Ana Aparecida Correa Xavier Flávia Helena Pereira Silva Priscilla Fajardo Valente Pereira Vitor Bruno Bianconi Rosa Karina Keller Marques da Costa Flaiban Giovana Wingeter Di Santis Thais Helena Constantino Patelli Júlio Augusto Naylor Lisbôa Amauri Alcindo Alfieri Clinical, epidemiological, and pathological findings of ovine gammaherpesvirus 2 infections in cattle from Southern Brazil Journal of Infection in Developing Countries Bovine gammaherpesvirus 6 Brachiaria spp poisoning diagnostic immunohistochemistry Macavirus malignant catarrhal fever |
| title | Clinical, epidemiological, and pathological findings of ovine gammaherpesvirus 2 infections in cattle from Southern Brazil |
| title_full | Clinical, epidemiological, and pathological findings of ovine gammaherpesvirus 2 infections in cattle from Southern Brazil |
| title_fullStr | Clinical, epidemiological, and pathological findings of ovine gammaherpesvirus 2 infections in cattle from Southern Brazil |
| title_full_unstemmed | Clinical, epidemiological, and pathological findings of ovine gammaherpesvirus 2 infections in cattle from Southern Brazil |
| title_short | Clinical, epidemiological, and pathological findings of ovine gammaherpesvirus 2 infections in cattle from Southern Brazil |
| title_sort | clinical epidemiological and pathological findings of ovine gammaherpesvirus 2 infections in cattle from southern brazil |
| topic | Bovine gammaherpesvirus 6 Brachiaria spp poisoning diagnostic immunohistochemistry Macavirus malignant catarrhal fever |
| url | https://jidc.org/index.php/journal/article/view/19951 |
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