A comparative analysis of mRNA enrichment strategies and guidance for improving their efficiency

A comparative analysis of mRNA enrichment strategies and guidance for improving their efficiency

Abstract The pervasive presence of ribosomal RNA (rRNA) in total RNA poses a considerable challenge to the accurate analysis of cellular transcriptomes. In this study, we comprehensively analyze strategies for Saccharomyces cerevisiae mRNA enrichment, aiming to either separate polyadenylated RNAs or...

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Bibliographic Details
Main Authors: Angelika Andrzejewska-Romanowska, Ewa Tykwińska, Paweł Śledziński, Katarzyna Pachulska-Wieczorek
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
Subjects:
mRNA enrichment
Ribosomal RNA depletion
Yeast transcriptome
Online Access:https://doi.org/10.1038/s41598-025-02082-z
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