Influence of B16/F10 melanoma growth variant on the level of cytochrome C in mitochondria in various organs of female mice
Introduction. Cytochrome C in mitochondria transfers electrons from complex III to complex IV, and it is a signaling molecule in the apoptosis realization.The objective was to evaluate the level of cytochrome C in cell mitochondria in various organs of female mice with standard and stimulated growth...
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Academician I.P. Pavlov First St. Petersburg State Medical University
2021-06-01
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| Series: | Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова |
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| Online Access: | https://www.sci-notes.ru/jour/article/view/774 |
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| author | E. M. Frantsiyants I. V. Neskubina N. D. Cheryarina E. I. Surikova L. A. Nemashkalova |
| author_facet | E. M. Frantsiyants I. V. Neskubina N. D. Cheryarina E. I. Surikova L. A. Nemashkalova |
| author_sort | E. M. Frantsiyants |
| collection | DOAJ |
| description | Introduction. Cytochrome C in mitochondria transfers electrons from complex III to complex IV, and it is a signaling molecule in the apoptosis realization.The objective was to evaluate the level of cytochrome C in cell mitochondria in various organs of female mice with standard and stimulated growth of experimental B16/F10 melanoma.Methods and materials. The experiment was performed on female C57BL/6 mice (n=168). The groups were: intact animals (n=21); controls with a model of chronic neurogenic pain (CNP) (n = 21); group M — standard B16/F10 melanoma transplantation (n=63), group CNP + M — B16/F10 melanoma transplantation 3 weeks after CNP model creation (n=63). The level of cytochrome C (ng / mg protein) were measured by ELISA (Bioscience, Austria). Statistical analysis of results was performed using the «Statistica 10.0» program.Results. After 1 week of standard melanoma growth, an increase in the level of cytochrome C by 2.7 and 1.7 times was detected in mitochondria of the brain and liver; by the 3rd week, it decreased in the liver and skin by 1.7 times. In melanoma mitochondria, the level of cytochrome C was lower than in the skin of intact animals: by 2.5 times after week 1, by 4.5 times after week 2, and by 4.6 times after week 3. After 1 week of stimulated melanoma growth, the level of cytochrome C decreased compared control values: by 2.2 times in the brain, by 1.9 times in the liver, by 1.4 times in the skin; by week 3, it decreased by 4.8 times in mitochondria of the brain, by 4.7 times — in the liver, by 2.3 times — in the heart, by 1.9 times — in the skin. In melanoma mitochondria, the level of cytochrome C was lower than in the skin of intact animals: by 15.3 times after week 1, by 10.3 times after week 2, and by 8.8 times after week 3.Conclusion. Low level of cytochrome C were found in melanoma mitochondria in standard and stimulated tumor growth. The data can be used in the experiment and in clinic for using exogenous cytochrome C as an agent slowing down the malignant process. |
| format | Article |
| id | doaj-art-9885b17c267c4f15bd20c7dfc229e952 |
| institution | DOAJ |
| issn | 1607-4181 2541-8807 |
| language | Russian |
| publishDate | 2021-06-01 |
| publisher | Academician I.P. Pavlov First St. Petersburg State Medical University |
| record_format | Article |
| series | Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова |
| spelling | doaj-art-9885b17c267c4f15bd20c7dfc229e9522025-08-20T03:21:33ZrusAcademician I.P. Pavlov First St. Petersburg State Medical UniversityУчёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова1607-41812541-88072021-06-01274465210.24884/1607-4181-2020-27-4-46-52537Influence of B16/F10 melanoma growth variant on the level of cytochrome C in mitochondria in various organs of female miceE. M. Frantsiyants0I. V. Neskubina1N. D. Cheryarina2E. I. Surikova3L. A. Nemashkalova4National Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyIntroduction. Cytochrome C in mitochondria transfers electrons from complex III to complex IV, and it is a signaling molecule in the apoptosis realization.The objective was to evaluate the level of cytochrome C in cell mitochondria in various organs of female mice with standard and stimulated growth of experimental B16/F10 melanoma.Methods and materials. The experiment was performed on female C57BL/6 mice (n=168). The groups were: intact animals (n=21); controls with a model of chronic neurogenic pain (CNP) (n = 21); group M — standard B16/F10 melanoma transplantation (n=63), group CNP + M — B16/F10 melanoma transplantation 3 weeks after CNP model creation (n=63). The level of cytochrome C (ng / mg protein) were measured by ELISA (Bioscience, Austria). Statistical analysis of results was performed using the «Statistica 10.0» program.Results. After 1 week of standard melanoma growth, an increase in the level of cytochrome C by 2.7 and 1.7 times was detected in mitochondria of the brain and liver; by the 3rd week, it decreased in the liver and skin by 1.7 times. In melanoma mitochondria, the level of cytochrome C was lower than in the skin of intact animals: by 2.5 times after week 1, by 4.5 times after week 2, and by 4.6 times after week 3. After 1 week of stimulated melanoma growth, the level of cytochrome C decreased compared control values: by 2.2 times in the brain, by 1.9 times in the liver, by 1.4 times in the skin; by week 3, it decreased by 4.8 times in mitochondria of the brain, by 4.7 times — in the liver, by 2.3 times — in the heart, by 1.9 times — in the skin. In melanoma mitochondria, the level of cytochrome C was lower than in the skin of intact animals: by 15.3 times after week 1, by 10.3 times after week 2, and by 8.8 times after week 3.Conclusion. Low level of cytochrome C were found in melanoma mitochondria in standard and stimulated tumor growth. The data can be used in the experiment and in clinic for using exogenous cytochrome C as an agent slowing down the malignant process.https://www.sci-notes.ru/jour/article/view/774cell mitochondriacytochrome cchronic neurogenic painexperimental b16/f10 melanomafemale mice |
| spellingShingle | E. M. Frantsiyants I. V. Neskubina N. D. Cheryarina E. I. Surikova L. A. Nemashkalova Influence of B16/F10 melanoma growth variant on the level of cytochrome C in mitochondria in various organs of female mice Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова cell mitochondria cytochrome c chronic neurogenic pain experimental b16/f10 melanoma female mice |
| title | Influence of B16/F10 melanoma growth variant on the level of cytochrome C in mitochondria in various organs of female mice |
| title_full | Influence of B16/F10 melanoma growth variant on the level of cytochrome C in mitochondria in various organs of female mice |
| title_fullStr | Influence of B16/F10 melanoma growth variant on the level of cytochrome C in mitochondria in various organs of female mice |
| title_full_unstemmed | Influence of B16/F10 melanoma growth variant on the level of cytochrome C in mitochondria in various organs of female mice |
| title_short | Influence of B16/F10 melanoma growth variant on the level of cytochrome C in mitochondria in various organs of female mice |
| title_sort | influence of b16 f10 melanoma growth variant on the level of cytochrome c in mitochondria in various organs of female mice |
| topic | cell mitochondria cytochrome c chronic neurogenic pain experimental b16/f10 melanoma female mice |
| url | https://www.sci-notes.ru/jour/article/view/774 |
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