Biodiversity and Hemolytic Toxicity of the Genus <i>Heterocapsa</i> (Dinophyceae) in the Beibu Gulf, China

Biodiversity and Hemolytic Toxicity of the Genus <i>Heterocapsa</i> (Dinophyceae) in the Beibu Gulf, China

The dinoflagellate genus <i>Heterocapsa</i> includes several widely distributed and potentially toxic species associated with Harmful Algal Blooms (HABs), particularly affecting the Western Pacific Ocean. To reveal the biodiversity of <i>Heterocapsa</i> in Beibu Gulf, six str...

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Bibliographic Details
Main Authors: Yixiao Xu, Nina Dzhembekova, Kirsty F. Smith, Haifeng Gu, Uwe John, Huanda Xie, Yujuan Wen, Miao Wu
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Marine Drugs
Subjects:
phylogeny
taxonomy
toxicity
erythrocyte lysis assay
dinoflagellate
harmful algal blooms
Online Access:https://www.mdpi.com/1660-3397/22/11/514
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Summary:The dinoflagellate genus <i>Heterocapsa</i> includes several widely distributed and potentially toxic species associated with Harmful Algal Blooms (HABs), particularly affecting the Western Pacific Ocean. To reveal the biodiversity of <i>Heterocapsa</i> in Beibu Gulf, six strains were morphologically characterized using light and scanning electron microscopy, while large subunit rDNA (LSU rDNA) and internal transcribed spacer (ITS) were sequenced for phylogenetic analysis through maximum likelihood and Bayesian inferences. Two strains (BGERL169, BGERL170) were identified as <i>Heterocapsa philippinensis</i> ribotype I, three (BGERL171-BGERL173) as a new <i>Heterocapsa philippinensis</i> ribotype II, and one strain (BGERL174) as <i>Heterocapsa pseudotriquetra</i>. Cells of <i>H. philippinensis</i> were ovoid to spherical, yellowish-brown, with reticulate chloroplasts, and had a sausage-shaped nucleus positioned longitudinally along the dorsal side of the cell, and the theca was arranged in Po, cp, X, 5′, 3a, 7″, 6c, 5s, 5‴, 2⁗. Additionally, BGERL169 and BGERL171 showed no hemolytic toxicity in rabbit erythrocyte lysis assays. To the best of our knowledge, this research provides the first morphological and phylogenetic analysis of <i>H. philippinensis</i>, including the identification of a new ribotype, as well as the discovery of <i>H. pseudotriquetra</i> in Chinese waters. The findings contribute to the understanding of <i>Heterocapsa</i> species biogeography and toxicity in Chinese waters, offering valuable data for future HAB monitoring in Beibu Gulf.
ISSN:1660-3397

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