Obtaining Immobilized Mycelium of Basidiomycete Fomitopsis Officinalis (Vill.:Fr.) Bond. Et Sing., Producer of Agaricic Acid
The article describes the development of a new method for obtaining mycelium of the basidiomycete Fomitopsis officinalis (Vill.:Fr.) Bond. Et Sing., immobilized on a matrix of bacterial cellulose. Mycelium of F.officinalis contains biologically active compounds, the most important of which is agaric...
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| Format: | Article |
| Language: | Russian |
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LLC "Publishing House OKI"
2020-05-01
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| Series: | Антибиотики и Химиотерапия |
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| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/176 |
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| author | T. I. Gromovykh I. A. Gavryushina V. S. Sadykova N. B. Feldman A. S. Dmitrenok A. Yu. Ayrapetova S. V. Lutsenko |
| author_facet | T. I. Gromovykh I. A. Gavryushina V. S. Sadykova N. B. Feldman A. S. Dmitrenok A. Yu. Ayrapetova S. V. Lutsenko |
| author_sort | T. I. Gromovykh |
| collection | DOAJ |
| description | The article describes the development of a new method for obtaining mycelium of the basidiomycete Fomitopsis officinalis (Vill.:Fr.) Bond. Et Sing., immobilized on a matrix of bacterial cellulose. Mycelium of F.officinalis contains biologically active compounds, the most important of which is agaricic acid. The known methods for producing mycelium using surface solid-phase and submerged cultivation make it possible to obtain biomass of mycelium in an amount of 3.5 to 5 g/L of absolutely dry mass. Considering that F.officinalis is a xylotrophic macromycete, the use of cellulose as a food source can show higher productivity in the cultivation of mycelium in artificial biotechnological systems. The aim of the work was to obtain immobilized mycelium by co-cultivation of F.officinalis with the producer of bacterial cellulose Gluconacetobacter hansenii. The research established that with the co-cultivation of the basidial strain of F.officinalis with G.hansenii, the producer strain of bacterial cellulose, the productivity increases by 3.2 times on synthetic medium H5/1 and on the natural medium Maltax-10 (5% concentration) — by 1.9 times. The obtained immobilized mycelium of F.officinalis contains agaricic acid, the amount of which is 5.4—6.8%. The study of the structure of agaricic acid, isolated from the mycelium of the strain, was carried out by comparing the 13C NMR spectra with the spectra of a standard sample (Sigma cat). The results of the research confirmed the identity of the compounds. |
| format | Article |
| id | doaj-art-9872dd7745d84dc19d517cb00ea6eb98 |
| institution | DOAJ |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2020-05-01 |
| publisher | LLC "Publishing House OKI" |
| record_format | Article |
| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-9872dd7745d84dc19d517cb00ea6eb982025-08-20T03:20:32ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902020-05-01639-1039175Obtaining Immobilized Mycelium of Basidiomycete Fomitopsis Officinalis (Vill.:Fr.) Bond. Et Sing., Producer of Agaricic AcidT. I. Gromovykh0I. A. Gavryushina1V. S. Sadykova2N. B. Feldman3A. S. Dmitrenok4A. Yu. Ayrapetova5S. V. Lutsenko6I. M. Sechenov First Moscow State Medical UniversityI. M. Sechenov First Moscow State Medical University; Gause Institute of New AntibioticsGause Institute of New AntibioticsI. M. Sechenov First Moscow State Medical UniversityN. D. Zelinsky Institute of Organic ChemistryРyatigorsk Medical Pharmaceutical Institute, branch of the Volgograd Medical State UniversityI. M. Sechenov First Moscow State Medical UniversityThe article describes the development of a new method for obtaining mycelium of the basidiomycete Fomitopsis officinalis (Vill.:Fr.) Bond. Et Sing., immobilized on a matrix of bacterial cellulose. Mycelium of F.officinalis contains biologically active compounds, the most important of which is agaricic acid. The known methods for producing mycelium using surface solid-phase and submerged cultivation make it possible to obtain biomass of mycelium in an amount of 3.5 to 5 g/L of absolutely dry mass. Considering that F.officinalis is a xylotrophic macromycete, the use of cellulose as a food source can show higher productivity in the cultivation of mycelium in artificial biotechnological systems. The aim of the work was to obtain immobilized mycelium by co-cultivation of F.officinalis with the producer of bacterial cellulose Gluconacetobacter hansenii. The research established that with the co-cultivation of the basidial strain of F.officinalis with G.hansenii, the producer strain of bacterial cellulose, the productivity increases by 3.2 times on synthetic medium H5/1 and on the natural medium Maltax-10 (5% concentration) — by 1.9 times. The obtained immobilized mycelium of F.officinalis contains agaricic acid, the amount of which is 5.4—6.8%. The study of the structure of agaricic acid, isolated from the mycelium of the strain, was carried out by comparing the 13C NMR spectra with the spectra of a standard sample (Sigma cat). The results of the research confirmed the identity of the compounds.https://www.antibiotics-chemotherapy.ru/jour/article/view/176fomitopsis officinalisgluconacetobacter hansenii |
| spellingShingle | T. I. Gromovykh I. A. Gavryushina V. S. Sadykova N. B. Feldman A. S. Dmitrenok A. Yu. Ayrapetova S. V. Lutsenko Obtaining Immobilized Mycelium of Basidiomycete Fomitopsis Officinalis (Vill.:Fr.) Bond. Et Sing., Producer of Agaricic Acid Антибиотики и Химиотерапия fomitopsis officinalis gluconacetobacter hansenii |
| title | Obtaining Immobilized Mycelium of Basidiomycete Fomitopsis Officinalis (Vill.:Fr.) Bond. Et Sing., Producer of Agaricic Acid |
| title_full | Obtaining Immobilized Mycelium of Basidiomycete Fomitopsis Officinalis (Vill.:Fr.) Bond. Et Sing., Producer of Agaricic Acid |
| title_fullStr | Obtaining Immobilized Mycelium of Basidiomycete Fomitopsis Officinalis (Vill.:Fr.) Bond. Et Sing., Producer of Agaricic Acid |
| title_full_unstemmed | Obtaining Immobilized Mycelium of Basidiomycete Fomitopsis Officinalis (Vill.:Fr.) Bond. Et Sing., Producer of Agaricic Acid |
| title_short | Obtaining Immobilized Mycelium of Basidiomycete Fomitopsis Officinalis (Vill.:Fr.) Bond. Et Sing., Producer of Agaricic Acid |
| title_sort | obtaining immobilized mycelium of basidiomycete fomitopsis officinalis vill fr bond et sing producer of agaricic acid |
| topic | fomitopsis officinalis gluconacetobacter hansenii |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/176 |
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