Obtaining Immobilized Mycelium of Basidiomycete Fomitopsis Officinalis (Vill.:Fr.) Bond. Et Sing., Producer of Agaricic Acid
The article describes the development of a new method for obtaining mycelium of the basidiomycete Fomitopsis officinalis (Vill.:Fr.) Bond. Et Sing., immobilized on a matrix of bacterial cellulose. Mycelium of F.officinalis contains biologically active compounds, the most important of which is agaric...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
LLC "Publishing House OKI"
2020-05-01
|
| Series: | Антибиотики и Химиотерапия |
| Subjects: | |
| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/176 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The article describes the development of a new method for obtaining mycelium of the basidiomycete Fomitopsis officinalis (Vill.:Fr.) Bond. Et Sing., immobilized on a matrix of bacterial cellulose. Mycelium of F.officinalis contains biologically active compounds, the most important of which is agaricic acid. The known methods for producing mycelium using surface solid-phase and submerged cultivation make it possible to obtain biomass of mycelium in an amount of 3.5 to 5 g/L of absolutely dry mass. Considering that F.officinalis is a xylotrophic macromycete, the use of cellulose as a food source can show higher productivity in the cultivation of mycelium in artificial biotechnological systems. The aim of the work was to obtain immobilized mycelium by co-cultivation of F.officinalis with the producer of bacterial cellulose Gluconacetobacter hansenii. The research established that with the co-cultivation of the basidial strain of F.officinalis with G.hansenii, the producer strain of bacterial cellulose, the productivity increases by 3.2 times on synthetic medium H5/1 and on the natural medium Maltax-10 (5% concentration) — by 1.9 times. The obtained immobilized mycelium of F.officinalis contains agaricic acid, the amount of which is 5.4—6.8%. The study of the structure of agaricic acid, isolated from the mycelium of the strain, was carried out by comparing the 13C NMR spectra with the spectra of a standard sample (Sigma cat). The results of the research confirmed the identity of the compounds. |
|---|---|
| ISSN: | 0235-2990 |