Investigation of Phagocyte Functions in Pseudomonas-Colonized Cystic Fibrosis Patients
Aim: Cystic fibrosis (CF) is an autosomal recessive disorder. Although it is considered as an epithelial disease due to impaired chloride transport, its pathogenesis remains unclear. CF is classified as a syndrome with congenital defects of phagocyte in recent human inborn errors of immunity phenoty...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2024-06-01
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| Series: | Journal of Pediatric Research |
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| Online Access: | https://jpedres.org/articles/investigation-of-phagocyte-functions-in-pseudomonas-colonized-cystic-fibrosis-patients/doi/jpr.galenos.2024.09735 |
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| author | Handan Duman Şenol Meral Barlık Ezgi Topyıldız Figen Gülen Güzide Aksu Necil Kütükçüler Esen Demir Neslihan Edeer Karaca |
| author_facet | Handan Duman Şenol Meral Barlık Ezgi Topyıldız Figen Gülen Güzide Aksu Necil Kütükçüler Esen Demir Neslihan Edeer Karaca |
| author_sort | Handan Duman Şenol |
| collection | DOAJ |
| description | Aim: Cystic fibrosis (CF) is an autosomal recessive disorder. Although it is considered as an epithelial disease due to impaired chloride transport, its pathogenesis remains unclear. CF is classified as a syndrome with congenital defects of phagocyte in recent human inborn errors of immunity phenotypic classifications. Neutrophils are the most effective cells in the eradication of bacterial infections such as Pseudomonas aeruginosa. The aim of the present study was to investigate the phagocyte functions in pseudomonas colonized CF patients.
Materials and Methods: A total number of 26 pseudomonas colonized CF patients and 21 healthy controls (gender and age matched) were included in this study. Absolute neutrophil counts (ANC), immunoglobulin values (Ig), the Migratest to evaluate chemotaxis in neutrophils and monocytes, CD11A/CD18/CD15 S (b2 integrin) adhesion molecules, and the Phagoburst test for intracellular bacterial killing were analyzed by flow cytometer.
Results: ANC, CD15S expression on neutrophils and IgG, IgA and IgM levels were higher in the CF patients than the control group (p<0.01). The neutrophils oxidative burst activity and the chemotactic ability of the CF patients did not differ from those of the controls. Patients with allergic bronchopulmonary aspergillosis and those with a mutation of 2183AA>G had significantly lower chemotaxis indexes than the others (p=0.01, p=0.01 respectively).
Conclusion: Our results from a small group of patients does not support impaired functions such as migration and phagocytosis of neutrophils in patients with CF. Further studies involving more CF patients are needed to make a definitive interpretation. |
| format | Article |
| id | doaj-art-986c02c509a449bbbb406115efa51d85 |
| institution | Kabale University |
| issn | 2147-9445 2587-2478 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Journal of Pediatric Research |
| spelling | doaj-art-986c02c509a449bbbb406115efa51d852025-08-20T03:37:23ZengGalenos Publishing HouseJournal of Pediatric Research2147-94452587-24782024-06-0111211011710.4274/jpr.galenos.2024.09735Investigation of Phagocyte Functions in Pseudomonas-Colonized Cystic Fibrosis PatientsHandan Duman Şenol0https://orcid.org/0000-0002-9519-8739Meral Barlık1https://orcid.org/0000-0003-1640-1733Ezgi Topyıldız2https://orcid.org/0000-0002-9260-8157Figen Gülen3https://orcid.org/0000-0002-5431-3913Güzide Aksu4https://orcid.org/0000-0003-2714-0903Necil Kütükçüler5https://orcid.org/0000-0002-9196-3819Esen Demir6https://orcid.org/0000-0003-2736-8924Neslihan Edeer Karaca7https://orcid.org/0000-0002-2202-7082Ege University Faculty of Medicine Department of Pediatric Allergy and Immunology, İzmir, TurkeyEge University Faculty of Medicine Department of Pediatric Pulmonology, İzmir, TurkeyEge University Faculty of Medicine Department of Pediatric Allergy and Immunology, İzmir, TurkeyEge University Faculty of Medicine Department of Pediatric Allergy and Immunology, İzmir, TurkeyEge University Faculty of Medicine Department of Pediatric Allergy and Immunology, İzmir, TurkeyEge University Faculty of Medicine Department of Pediatric Allergy and Immunology, İzmir, TurkeyEge University Faculty of Medicine Department of Pediatric Allergy and Immunology, İzmir, TurkeyEge University Faculty of Medicine Department of Pediatric Allergy and Immunology, İzmir, TurkeyAim: Cystic fibrosis (CF) is an autosomal recessive disorder. Although it is considered as an epithelial disease due to impaired chloride transport, its pathogenesis remains unclear. CF is classified as a syndrome with congenital defects of phagocyte in recent human inborn errors of immunity phenotypic classifications. Neutrophils are the most effective cells in the eradication of bacterial infections such as Pseudomonas aeruginosa. The aim of the present study was to investigate the phagocyte functions in pseudomonas colonized CF patients. Materials and Methods: A total number of 26 pseudomonas colonized CF patients and 21 healthy controls (gender and age matched) were included in this study. Absolute neutrophil counts (ANC), immunoglobulin values (Ig), the Migratest to evaluate chemotaxis in neutrophils and monocytes, CD11A/CD18/CD15 S (b2 integrin) adhesion molecules, and the Phagoburst test for intracellular bacterial killing were analyzed by flow cytometer. Results: ANC, CD15S expression on neutrophils and IgG, IgA and IgM levels were higher in the CF patients than the control group (p<0.01). The neutrophils oxidative burst activity and the chemotactic ability of the CF patients did not differ from those of the controls. Patients with allergic bronchopulmonary aspergillosis and those with a mutation of 2183AA>G had significantly lower chemotaxis indexes than the others (p=0.01, p=0.01 respectively). Conclusion: Our results from a small group of patients does not support impaired functions such as migration and phagocytosis of neutrophils in patients with CF. Further studies involving more CF patients are needed to make a definitive interpretation.https://jpedres.org/articles/investigation-of-phagocyte-functions-in-pseudomonas-colonized-cystic-fibrosis-patients/doi/jpr.galenos.2024.09735cystic fibrosischemotaxisphagocytosisneutrophil functionadhesion molecule |
| spellingShingle | Handan Duman Şenol Meral Barlık Ezgi Topyıldız Figen Gülen Güzide Aksu Necil Kütükçüler Esen Demir Neslihan Edeer Karaca Investigation of Phagocyte Functions in Pseudomonas-Colonized Cystic Fibrosis Patients Journal of Pediatric Research cystic fibrosis chemotaxis phagocytosis neutrophil function adhesion molecule |
| title | Investigation of Phagocyte Functions in Pseudomonas-Colonized Cystic Fibrosis Patients |
| title_full | Investigation of Phagocyte Functions in Pseudomonas-Colonized Cystic Fibrosis Patients |
| title_fullStr | Investigation of Phagocyte Functions in Pseudomonas-Colonized Cystic Fibrosis Patients |
| title_full_unstemmed | Investigation of Phagocyte Functions in Pseudomonas-Colonized Cystic Fibrosis Patients |
| title_short | Investigation of Phagocyte Functions in Pseudomonas-Colonized Cystic Fibrosis Patients |
| title_sort | investigation of phagocyte functions in pseudomonas colonized cystic fibrosis patients |
| topic | cystic fibrosis chemotaxis phagocytosis neutrophil function adhesion molecule |
| url | https://jpedres.org/articles/investigation-of-phagocyte-functions-in-pseudomonas-colonized-cystic-fibrosis-patients/doi/jpr.galenos.2024.09735 |
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