Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal study
Abstract Background Several biomaterials have been employed for direct pulp capping (DPC) with varying degrees of success. This study evaluated the pulp response following DPC with a new material developed from glycogen synthase kinase-3 inhibitors (Tideglusib) and mineral trioxide aggregate (MTA)....
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BMC
2025-07-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-025-06546-6 |
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| author | Momen M. Mhmod Ehab E. Hassanien Ashraf M. Abu-Seida Salma H. EL Ashry Mohamed M. Nagy Sara H. Fahmy Elhassan E.E. Hassanein |
| author_facet | Momen M. Mhmod Ehab E. Hassanien Ashraf M. Abu-Seida Salma H. EL Ashry Mohamed M. Nagy Sara H. Fahmy Elhassan E.E. Hassanein |
| author_sort | Momen M. Mhmod |
| collection | DOAJ |
| description | Abstract Background Several biomaterials have been employed for direct pulp capping (DPC) with varying degrees of success. This study evaluated the pulp response following DPC with a new material developed from glycogen synthase kinase-3 inhibitors (Tideglusib) and mineral trioxide aggregate (MTA). Methods Class V cavities with pulp exposure were conducted on 56 teeth in two adult male mongrel dogs. Based on the evaluation periods, these teeth were divided into two major groups at random (28 teeth/dog each). Groups A and B underwent histopathology evaluations three and eight weeks following DPC, respectively. Depending on the capping material used, each main group was further divided into two equal subgroups (14 teeth each). ProRoot white MTA was applied directly to the exposed vital pulps in subgroup 1. While subgroup 2’s exposed pulps were immediately capped with resorbable collagen that had been soaked in a freshly made 50 nM Tideglusib drug solution. Glass ionomer filling was then used to seal the access cavities. Every specimen underwent histological evaluation and was scored according to the number of inflammatory cells, the disorganization of the pulp tissue, and the formation of calcific bridges. All data were statistically examined. Results In both groups A and B, subgroup 2 showed a statistically significant increase in the number of inflammatory cells and pulp tissue disorganization compared to subgroup 1 (P < 0.05). In both groups A and B, there was no statistically significant difference in the formation of new hard tissue between subgroups 1 and 2 (P = 0.157). Conclusion When used as direct vital pulp capping materials in a dog model, Tideglusib causes more soft tissue disorganization and an inflammatory response inside the pulp cavity than ProRoot white MTA. |
| format | Article |
| id | doaj-art-9868e5a1fe7641708ebb86917e3c1f16 |
| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-9868e5a1fe7641708ebb86917e3c1f162025-08-20T03:43:37ZengBMCBMC Oral Health1472-68312025-07-0125111010.1186/s12903-025-06546-6Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal studyMomen M. Mhmod0Ehab E. Hassanien1Ashraf M. Abu-Seida2Salma H. EL Ashry3Mohamed M. Nagy4Sara H. Fahmy5Elhassan E.E. Hassanein6Endodontic Department, Faculty of Dentistry, October University for Modern Sciences & ArtsEndodontic Department, Faculty of Dentistry, Galala UniversityDepartment of Surgery, Anesthesiology & Radiology, Faculty of Veterinary Medicine, Cairo UniversityEndodontic Department, Faculty of Dentistry, Ain Shams UniversityEndodontic Department, Faculty of Dentistry, Galala UniversityEndodontic Department, Faculty of Dentistry, Ain Shams UniversityHHU Heinrich Heine Universität DüsseldorfAbstract Background Several biomaterials have been employed for direct pulp capping (DPC) with varying degrees of success. This study evaluated the pulp response following DPC with a new material developed from glycogen synthase kinase-3 inhibitors (Tideglusib) and mineral trioxide aggregate (MTA). Methods Class V cavities with pulp exposure were conducted on 56 teeth in two adult male mongrel dogs. Based on the evaluation periods, these teeth were divided into two major groups at random (28 teeth/dog each). Groups A and B underwent histopathology evaluations three and eight weeks following DPC, respectively. Depending on the capping material used, each main group was further divided into two equal subgroups (14 teeth each). ProRoot white MTA was applied directly to the exposed vital pulps in subgroup 1. While subgroup 2’s exposed pulps were immediately capped with resorbable collagen that had been soaked in a freshly made 50 nM Tideglusib drug solution. Glass ionomer filling was then used to seal the access cavities. Every specimen underwent histological evaluation and was scored according to the number of inflammatory cells, the disorganization of the pulp tissue, and the formation of calcific bridges. All data were statistically examined. Results In both groups A and B, subgroup 2 showed a statistically significant increase in the number of inflammatory cells and pulp tissue disorganization compared to subgroup 1 (P < 0.05). In both groups A and B, there was no statistically significant difference in the formation of new hard tissue between subgroups 1 and 2 (P = 0.157). Conclusion When used as direct vital pulp capping materials in a dog model, Tideglusib causes more soft tissue disorganization and an inflammatory response inside the pulp cavity than ProRoot white MTA.https://doi.org/10.1186/s12903-025-06546-6Dentin repairMineral trioxide aggregatePulp inflammationTideglusibVital pulp therapyWnt/β-catenin signaling pathway |
| spellingShingle | Momen M. Mhmod Ehab E. Hassanien Ashraf M. Abu-Seida Salma H. EL Ashry Mohamed M. Nagy Sara H. Fahmy Elhassan E.E. Hassanein Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal study BMC Oral Health Dentin repair Mineral trioxide aggregate Pulp inflammation Tideglusib Vital pulp therapy Wnt/β-catenin signaling pathway |
| title | Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal study |
| title_full | Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal study |
| title_fullStr | Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal study |
| title_full_unstemmed | Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal study |
| title_short | Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal study |
| title_sort | pulp response following direct pulp capping with tideglusib and mineral trioxide aggregate an animal study |
| topic | Dentin repair Mineral trioxide aggregate Pulp inflammation Tideglusib Vital pulp therapy Wnt/β-catenin signaling pathway |
| url | https://doi.org/10.1186/s12903-025-06546-6 |
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