Protocol to study ductal progenitor-like cells from the adult human pancreas using 3D suspension and methylcellulose-based culture systems

Protocol to study ductal progenitor-like cells from the adult human pancreas using 3D suspension and methylcellulose-based culture systems

Summary: Primary human ductal progenitor-like cells derived from donated pancreas have the potential to serve as a source of therapeutic insulin-producing beta cells for the treatment of diabetes. Here, we present a protocol for studying ductal progenitor-like cells using a good manufacturing practi...

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Bibliographic Details
Main Authors: Heather N. Zook, Janine C. Quijano, Jose A. Ortiz, Cecile Donohue, Neslihan Erdem, Hsun Teresa Ku
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002539
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