Cigarette and e-cigarette reversely regulated autophagy via distinct level of ROS in vitro
With the increasing prevalence of e-cigarettes, a systematic evaluation of the cytotoxicity among the components of e-cigarettes is needed. This study aimed to compare the cytotoxicity of conventional cigarettes and e-cigarettes, identify the toxic components of e-cigarettes, and explore the mechani...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015240 |
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| author | Yao Wang Jialing Zhong Zuyi Jin An Luo Zehong Wu Xingtao Jiang Jianwen Chen Peiqing Liu Min Li |
| author_facet | Yao Wang Jialing Zhong Zuyi Jin An Luo Zehong Wu Xingtao Jiang Jianwen Chen Peiqing Liu Min Li |
| author_sort | Yao Wang |
| collection | DOAJ |
| description | With the increasing prevalence of e-cigarettes, a systematic evaluation of the cytotoxicity among the components of e-cigarettes is needed. This study aimed to compare the cytotoxicity of conventional cigarettes and e-cigarettes, identify the toxic components of e-cigarettes, and explore the mechanisms of cellular injury. In this study, the conventional cigarette and seven flavored e-cigarettes, along with their constituents, including solvents, nicotine, and seven flavoring agents were tested. The Cambridge filter pad method was employed to collect conventional cigarettes smoke condensate (CSC) and e-cigarettes aerosol condensate (EAC). CCK-8 assays were used to assess their cytotoxicity on respiratory system-related cells and cardiovascular-related cells. The findings revealed significantly higher cytotoxicity in CSC compared to EACs, with EACs exhibiting IC50 values at least 12 times higher across various flavors and cell lines. Notably, among the seven flavors, the Cola-flavored EAC exhibited the most pronounced cytotoxicity, with flavoring agents identified as the primary contributors to the cytotoxicity of e-cigarettes. Furthermore, noteworthy differences in cellular mechanisms were observed between CSC and EAC-Cola in BEAS-2B cells, particularly in terms of ROS level and autophagy regulation. CSC induced approximately 10 times higher levels of ROS compared to EAC. Due to the distinct level of ROS, CSC significantly inhibited autophagy while EAC induced autophagy. Scavenge of ROS clearly eliminates the autophagy modulated by both CSC and EAC. These findings contribute to a comprehensive understanding of the cytotoxicity of e-cigarettes and conventional cigarettes, providing a scientific basis for health risk assessment and regulatory mechanisms. |
| format | Article |
| id | doaj-art-984a8240acb34bafa20c743b174fc911 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-984a8240acb34bafa20c743b174fc9112025-01-23T05:25:36ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117448Cigarette and e-cigarette reversely regulated autophagy via distinct level of ROS in vitroYao Wang0Jialing Zhong1Zuyi Jin2An Luo3Zehong Wu4Xingtao Jiang5Jianwen Chen6Peiqing Liu7Min Li8School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, National and Local Joint Engineering Laboratory of Druggability and New Drugs Evaluation, Sun Yat-Sen University, Guangzhou, Guangdong 510006, ChinaSchool of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, National and Local Joint Engineering Laboratory of Druggability and New Drugs Evaluation, Sun Yat-Sen University, Guangzhou, Guangdong 510006, ChinaSchool of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, National and Local Joint Engineering Laboratory of Druggability and New Drugs Evaluation, Sun Yat-Sen University, Guangzhou, Guangdong 510006, ChinaRELX Lab, Shenzhen RELX Tech. Co. Ltd., Shenzhen, Guangdong 518000, ChinaRELX Lab, Shenzhen RELX Tech. Co. Ltd., Shenzhen, Guangdong 518000, ChinaRELX Lab, Shenzhen RELX Tech. Co. Ltd., Shenzhen, Guangdong 518000, ChinaSchool of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, National and Local Joint Engineering Laboratory of Druggability and New Drugs Evaluation, Sun Yat-Sen University, Guangzhou, Guangdong 510006, ChinaSchool of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, National and Local Joint Engineering Laboratory of Druggability and New Drugs Evaluation, Sun Yat-Sen University, Guangzhou, Guangdong 510006, ChinaSchool of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, National and Local Joint Engineering Laboratory of Druggability and New Drugs Evaluation, Sun Yat-Sen University, Guangzhou, Guangdong 510006, China; Corresponding author.With the increasing prevalence of e-cigarettes, a systematic evaluation of the cytotoxicity among the components of e-cigarettes is needed. This study aimed to compare the cytotoxicity of conventional cigarettes and e-cigarettes, identify the toxic components of e-cigarettes, and explore the mechanisms of cellular injury. In this study, the conventional cigarette and seven flavored e-cigarettes, along with their constituents, including solvents, nicotine, and seven flavoring agents were tested. The Cambridge filter pad method was employed to collect conventional cigarettes smoke condensate (CSC) and e-cigarettes aerosol condensate (EAC). CCK-8 assays were used to assess their cytotoxicity on respiratory system-related cells and cardiovascular-related cells. The findings revealed significantly higher cytotoxicity in CSC compared to EACs, with EACs exhibiting IC50 values at least 12 times higher across various flavors and cell lines. Notably, among the seven flavors, the Cola-flavored EAC exhibited the most pronounced cytotoxicity, with flavoring agents identified as the primary contributors to the cytotoxicity of e-cigarettes. Furthermore, noteworthy differences in cellular mechanisms were observed between CSC and EAC-Cola in BEAS-2B cells, particularly in terms of ROS level and autophagy regulation. CSC induced approximately 10 times higher levels of ROS compared to EAC. Due to the distinct level of ROS, CSC significantly inhibited autophagy while EAC induced autophagy. Scavenge of ROS clearly eliminates the autophagy modulated by both CSC and EAC. These findings contribute to a comprehensive understanding of the cytotoxicity of e-cigarettes and conventional cigarettes, providing a scientific basis for health risk assessment and regulatory mechanisms.http://www.sciencedirect.com/science/article/pii/S0147651324015240Cigarette smokeE-cigarette aerosolFlavoring agentsROSAutophagy |
| spellingShingle | Yao Wang Jialing Zhong Zuyi Jin An Luo Zehong Wu Xingtao Jiang Jianwen Chen Peiqing Liu Min Li Cigarette and e-cigarette reversely regulated autophagy via distinct level of ROS in vitro Ecotoxicology and Environmental Safety Cigarette smoke E-cigarette aerosol Flavoring agents ROS Autophagy |
| title | Cigarette and e-cigarette reversely regulated autophagy via distinct level of ROS in vitro |
| title_full | Cigarette and e-cigarette reversely regulated autophagy via distinct level of ROS in vitro |
| title_fullStr | Cigarette and e-cigarette reversely regulated autophagy via distinct level of ROS in vitro |
| title_full_unstemmed | Cigarette and e-cigarette reversely regulated autophagy via distinct level of ROS in vitro |
| title_short | Cigarette and e-cigarette reversely regulated autophagy via distinct level of ROS in vitro |
| title_sort | cigarette and e cigarette reversely regulated autophagy via distinct level of ros in vitro |
| topic | Cigarette smoke E-cigarette aerosol Flavoring agents ROS Autophagy |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015240 |
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