Genome-Wide Identification and Expression Analysis of <i>NAC</i> Gene Family Members in Seashore Paspalum Under Salt Stress
The <i>NAC</i> gene family plays a crucial role in plant growth, development, and responses to biotic and abiotic stresses. <i>Paspalum Vaginatum</i>, a warm-season turfgrass with exceptional salt tolerance, can be irrigated with seawater. However, the <i>NAC</i>...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/13/24/3595 |
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| Summary: | The <i>NAC</i> gene family plays a crucial role in plant growth, development, and responses to biotic and abiotic stresses. <i>Paspalum Vaginatum</i>, a warm-season turfgrass with exceptional salt tolerance, can be irrigated with seawater. However, the <i>NAC</i> gene family in seashore paspalum remains poorly understood. In this study, genome-wide screening and identification were conducted based on the <i>NAC</i> (<i>NAM</i>) domain hidden Markov model in seashore paspalum, resulting in the identification of 168 <i>PvNAC</i> genes. A phylogenetic tree was constructed, and the genes were classified into 18 groups according to their topological structure. The physicochemical properties of the <i>PvNAC</i> gene family proteins, their conserved motifs and structural domains, cis-acting elements, intraspecific collinearity analysis, GO annotation analysis, and protein–protein interaction networks were analyzed. The results indicated that the majority of PvNAC proteins are hydrophilic and predominantly localized in the nucleus. The promoter regions of <i>PvNACs</i> are primarily enriched with light-responsive elements, <i>ABRE</i> motifs, <i>MYB</i> motifs, and others. Intraspecific collinearity analysis suggests that <i>PvNACs</i> may have experienced a large-scale gene duplication event. GO annotation indicated that <i>PvNAC</i> genes were essential for transcriptional regulation, organ development, and responses to environmental stimuli. Furthermore, the protein interaction network predicted that <i>PvNAC73</i> interacts with proteins such as <i>BZIP8</i> and <i>DREB2A</i> to form a major regulatory hub. The transcriptomic analysis investigates the expression patterns of <i>NAC</i> genes in both leaves and roots under varying durations of salt stress. The expression levels of 8 <i>PvNACs</i> in roots and leaves under salt stress were examined and increased to varying degrees under salt stress. The qRT-PCR results demonstrated that the expression levels of the selected genes were consistent with the FPKM value trends observed in the RNA-seq data. This study established a theoretical basis for understanding the molecular functions and regulatory mechanisms of the <i>NAC</i> gene family in seashore paspalum under salt stress. |
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| ISSN: | 2223-7747 |