Molecular identification of the medicinal and edible plant Spatholobus suberectus Dunn and its counterfeits and adulterants based on rbcL barcode
The study aimed to compare and analyze the ribulose-1, 5-bisphosphate carboxylase (rbcL) sequences of Spatholobus suberectus Dunn and its adulterants and to explore whether rbcL could be used as the DNA barcode to effectively identify them. The obtained sample rbcL sequences were compared with those...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | CyTA - Journal of Food |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2025.2505683 |
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| author | Jiawen Wu Yunfeng Huang Renchuan Hu Qimin Hu Chuangui Xu Kedao Lai Ziyi Zhao |
| author_facet | Jiawen Wu Yunfeng Huang Renchuan Hu Qimin Hu Chuangui Xu Kedao Lai Ziyi Zhao |
| author_sort | Jiawen Wu |
| collection | DOAJ |
| description | The study aimed to compare and analyze the ribulose-1, 5-bisphosphate carboxylase (rbcL) sequences of Spatholobus suberectus Dunn and its adulterants and to explore whether rbcL could be used as the DNA barcode to effectively identify them. The obtained sample rbcL sequences were compared with those in the NCBI database, and the alignment results were consistent with the morphological identification results. The phylogenetic tree shows that the rbcL sequence of S. suberectus clusters as a distinct branch, clearly distinguishing it from other species. The K2P genetic distance analysis reveals that the average intra-species genetic distance of S. suberectus is smaller than the average inter-species genetic distance between S. suberectus and other species, indicating a clear genetic divergence between S. suberectus and other species based on the rbcL sequence. In conclusion, the rbcL barcode is an efficient and accurate DNA barcode for distinguishing S. suberectus from its adulterants. |
| format | Article |
| id | doaj-art-9845f63f28e94b5087c5afee9232c2fc |
| institution | OA Journals |
| issn | 1947-6337 1947-6345 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj-art-9845f63f28e94b5087c5afee9232c2fc2025-08-20T02:30:47ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452025-12-0123110.1080/19476337.2025.2505683Molecular identification of the medicinal and edible plant Spatholobus suberectus Dunn and its counterfeits and adulterants based on rbcL barcodeJiawen Wu0Yunfeng Huang1Renchuan Hu2Qimin Hu3Chuangui Xu4Kedao Lai5Ziyi Zhao6College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, ChinaGuangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, ChinaGuangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, ChinaGuangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, ChinaGuangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, ChinaGuangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, ChinaGuangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, ChinaThe study aimed to compare and analyze the ribulose-1, 5-bisphosphate carboxylase (rbcL) sequences of Spatholobus suberectus Dunn and its adulterants and to explore whether rbcL could be used as the DNA barcode to effectively identify them. The obtained sample rbcL sequences were compared with those in the NCBI database, and the alignment results were consistent with the morphological identification results. The phylogenetic tree shows that the rbcL sequence of S. suberectus clusters as a distinct branch, clearly distinguishing it from other species. The K2P genetic distance analysis reveals that the average intra-species genetic distance of S. suberectus is smaller than the average inter-species genetic distance between S. suberectus and other species, indicating a clear genetic divergence between S. suberectus and other species based on the rbcL sequence. In conclusion, the rbcL barcode is an efficient and accurate DNA barcode for distinguishing S. suberectus from its adulterants.https://www.tandfonline.com/doi/10.1080/19476337.2025.2505683Spatholobus suberectusrbcLDNA barcodingcounterfeits and adulterantsmolecular identification |
| spellingShingle | Jiawen Wu Yunfeng Huang Renchuan Hu Qimin Hu Chuangui Xu Kedao Lai Ziyi Zhao Molecular identification of the medicinal and edible plant Spatholobus suberectus Dunn and its counterfeits and adulterants based on rbcL barcode CyTA - Journal of Food Spatholobus suberectus rbcL DNA barcoding counterfeits and adulterants molecular identification |
| title | Molecular identification of the medicinal and edible plant Spatholobus suberectus Dunn and its counterfeits and adulterants based on rbcL barcode |
| title_full | Molecular identification of the medicinal and edible plant Spatholobus suberectus Dunn and its counterfeits and adulterants based on rbcL barcode |
| title_fullStr | Molecular identification of the medicinal and edible plant Spatholobus suberectus Dunn and its counterfeits and adulterants based on rbcL barcode |
| title_full_unstemmed | Molecular identification of the medicinal and edible plant Spatholobus suberectus Dunn and its counterfeits and adulterants based on rbcL barcode |
| title_short | Molecular identification of the medicinal and edible plant Spatholobus suberectus Dunn and its counterfeits and adulterants based on rbcL barcode |
| title_sort | molecular identification of the medicinal and edible plant spatholobus suberectus dunn and its counterfeits and adulterants based on rbcl barcode |
| topic | Spatholobus suberectus rbcL DNA barcoding counterfeits and adulterants molecular identification |
| url | https://www.tandfonline.com/doi/10.1080/19476337.2025.2505683 |
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