Compositional variation in eye-infiltrating immune cells distinguishes human uveitis subtypes

Summary: Uveitis is a heterogeneous group of ocular inflammatory diseases that respond inadequately to empiric therapy, underscoring the need to define pathophysiologic subtypes. Toward this goal, we applied single-cell transcriptional and immune receptor profiling to ocular fluid biopsies from 23 p...

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Main Authors: Christian Concepcion, Yu Xia, Yulia Korshunova, Gregory W. Bligard, Amal Taylor, Michael A. Paley, Philip A. Ruzycki, Lynn M. Hassman
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:iScience
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Online Access:http://www.sciencedirect.com/science/article/pii/S2589004225001889
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author Christian Concepcion
Yu Xia
Yulia Korshunova
Gregory W. Bligard
Amal Taylor
Michael A. Paley
Philip A. Ruzycki
Lynn M. Hassman
author_facet Christian Concepcion
Yu Xia
Yulia Korshunova
Gregory W. Bligard
Amal Taylor
Michael A. Paley
Philip A. Ruzycki
Lynn M. Hassman
author_sort Christian Concepcion
collection DOAJ
description Summary: Uveitis is a heterogeneous group of ocular inflammatory diseases that respond inadequately to empiric therapy, underscoring the need to define pathophysiologic subtypes. Toward this goal, we applied single-cell transcriptional and immune receptor profiling to ocular fluid biopsies from 23 patients with diverse clinical features. We found that a subset of ocular T cells undergoes local clonal expansion, indicating that antigens trigger uveitis in some patients. Infiltrates from HLA-B27 associated acute anterior uveitis (HLA-B27 AAU) were enriched in myeloid cells and innate immune signaling pathways, while chronic uveitis featured prominent adaptive immune cells and transcriptional programs, indicating that immunologic features distinguish uveitis subtypes. Clinical features including anatomic involvement were associated with additional variability in the immune cell profile. These insights highlight the potential for deep transcriptional profiling of ocular immune cells to reveal endotypes within uveitis.
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institution DOAJ
issn 2589-0042
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publishDate 2025-03-01
publisher Elsevier
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series iScience
spelling doaj-art-9840f0eccc3c49c6a220cf26c4f1c75d2025-08-20T03:00:35ZengElsevieriScience2589-00422025-03-0128311192810.1016/j.isci.2025.111928Compositional variation in eye-infiltrating immune cells distinguishes human uveitis subtypesChristian Concepcion0Yu Xia1Yulia Korshunova2Gregory W. Bligard3Amal Taylor4Michael A. Paley5Philip A. Ruzycki6Lynn M. Hassman7Department of Ophthalmology, University of Colorado, Aurora, CO 80045, USA; Department of Ophthalmology and Visual Sciences, Washington University in St. Louis, St. Louis, MO 63108, USADepartment of Ophthalmology and Visual Sciences, Washington University in St. Louis, St. Louis, MO 63108, USADepartment of Ophthalmology and Visual Sciences, Washington University in St. Louis, St. Louis, MO 63108, USADepartment of Ophthalmology and Visual Sciences, Washington University in St. Louis, St. Louis, MO 63108, USADepartment of Ophthalmology and Visual Sciences, Washington University in St. Louis, St. Louis, MO 63108, USADepartment of Medicine, Washington University in St. Louis, St. Louis, MO 63108, USADepartment of Ophthalmology and Visual Sciences, Washington University in St. Louis, St. Louis, MO 63108, USADepartment of Ophthalmology, University of Colorado, Aurora, CO 80045, USA; Department of Ophthalmology and Visual Sciences, Washington University in St. Louis, St. Louis, MO 63108, USA; Corresponding authorSummary: Uveitis is a heterogeneous group of ocular inflammatory diseases that respond inadequately to empiric therapy, underscoring the need to define pathophysiologic subtypes. Toward this goal, we applied single-cell transcriptional and immune receptor profiling to ocular fluid biopsies from 23 patients with diverse clinical features. We found that a subset of ocular T cells undergoes local clonal expansion, indicating that antigens trigger uveitis in some patients. Infiltrates from HLA-B27 associated acute anterior uveitis (HLA-B27 AAU) were enriched in myeloid cells and innate immune signaling pathways, while chronic uveitis featured prominent adaptive immune cells and transcriptional programs, indicating that immunologic features distinguish uveitis subtypes. Clinical features including anatomic involvement were associated with additional variability in the immune cell profile. These insights highlight the potential for deep transcriptional profiling of ocular immune cells to reveal endotypes within uveitis.http://www.sciencedirect.com/science/article/pii/S2589004225001889Cell biologyImmunityTranscriptomics
spellingShingle Christian Concepcion
Yu Xia
Yulia Korshunova
Gregory W. Bligard
Amal Taylor
Michael A. Paley
Philip A. Ruzycki
Lynn M. Hassman
Compositional variation in eye-infiltrating immune cells distinguishes human uveitis subtypes
iScience
Cell biology
Immunity
Transcriptomics
title Compositional variation in eye-infiltrating immune cells distinguishes human uveitis subtypes
title_full Compositional variation in eye-infiltrating immune cells distinguishes human uveitis subtypes
title_fullStr Compositional variation in eye-infiltrating immune cells distinguishes human uveitis subtypes
title_full_unstemmed Compositional variation in eye-infiltrating immune cells distinguishes human uveitis subtypes
title_short Compositional variation in eye-infiltrating immune cells distinguishes human uveitis subtypes
title_sort compositional variation in eye infiltrating immune cells distinguishes human uveitis subtypes
topic Cell biology
Immunity
Transcriptomics
url http://www.sciencedirect.com/science/article/pii/S2589004225001889
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