Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis
ABSTRACT The evidence that intratumoral microbiomes, as a rising hallmark of cancer, have a profound impact on cancer phenotypes is increasingly compelling. However, the impact of the composition and diversity of the intratumoral microbiome on the prognosis of patients undergoing surgical resection...
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| Language: | English |
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American Society for Microbiology
2025-01-01
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| Series: | mSystems |
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| Online Access: | https://journals.asm.org/doi/10.1128/msystems.00765-24 |
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| author | Fengle Jiang Yuan Dang Zheting Zhang Yanan Yan Yingchao Wang Yi Chen Lihong Chen Jialiang Zhang Jingfeng Liu Jianmin Wang |
| author_facet | Fengle Jiang Yuan Dang Zheting Zhang Yanan Yan Yingchao Wang Yi Chen Lihong Chen Jialiang Zhang Jingfeng Liu Jianmin Wang |
| author_sort | Fengle Jiang |
| collection | DOAJ |
| description | ABSTRACT The evidence that intratumoral microbiomes, as a rising hallmark of cancer, have a profound impact on cancer phenotypes is increasingly compelling. However, the impact of the composition and diversity of the intratumoral microbiome on the prognosis of patients undergoing surgical resection for hepatocellular carcinoma (HCC) remains incompletely understood. In this study, we revealed a high abundance of bacteria in the neoplastic tissues. The presence of bacterial lipopolysaccharide and lipoteichoic acid was detected alongside tumor-associated immune cells. By utilizing 16S rRNA gene sequencing, we identified a specific intratumoral microbiome signature that was highly predictive of the prognosis for HCC patients who underwent surgical resection. Specifically, the presence of Intestinimonas, Brachybacterium, and Rothia were identified as independent risk factors for the overall survival of HCC patients who underwent surgical resection.IMPORTANCEAlthough some studies have shown an abundance of bacteria in hepatocellular carcinoma (HCC), there is still limited understanding of the composition and diversity of the intratumoral microbiome that is favorable or adverse to the prognosis of HCC patients. Our results indicated that a greater abundance of bacteria could be observed in the neoplastic tissues than in nonneoplastic tissues. Bacterial cell wall components largely coincided with tumor-associated immune cells. The bacteria in the long overall survival (LOS) group were associated with metabolism and cytokine‒cytokine receptor interaction pathways, while bacteria in the short overall survival (SOS) group were associated with proinflammatory and cell proliferation pathways. Notably, specific taxa could independently predict HCC prognosis. Based on these findings, intratumoral microbiomes facilitate the use of precision medicine in clinical practice. |
| format | Article |
| id | doaj-art-983c858827774151b1462bc48bad95aa |
| institution | Kabale University |
| issn | 2379-5077 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSystems |
| spelling | doaj-art-983c858827774151b1462bc48bad95aa2025-01-21T14:00:28ZengAmerican Society for MicrobiologymSystems2379-50772025-01-0110110.1128/msystems.00765-24Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosisFengle Jiang0Yuan Dang1Zheting Zhang2Yanan Yan3Yingchao Wang4Yi Chen5Lihong Chen6Jialiang Zhang7Jingfeng Liu8Jianmin Wang9Innovation Center for Cancer Research, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, ChinaInnovation Center for Cancer Research, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, ChinaInnovation Center for Cancer Research, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, ChinaInnovation Center for Cancer Research, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, ChinaThe United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, ChinaThe United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, ChinaThe United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, ChinaInnovation Center for Cancer Research, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, ChinaInnovation Center for Cancer Research, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, ChinaInnovation Center for Cancer Research, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, ChinaABSTRACT The evidence that intratumoral microbiomes, as a rising hallmark of cancer, have a profound impact on cancer phenotypes is increasingly compelling. However, the impact of the composition and diversity of the intratumoral microbiome on the prognosis of patients undergoing surgical resection for hepatocellular carcinoma (HCC) remains incompletely understood. In this study, we revealed a high abundance of bacteria in the neoplastic tissues. The presence of bacterial lipopolysaccharide and lipoteichoic acid was detected alongside tumor-associated immune cells. By utilizing 16S rRNA gene sequencing, we identified a specific intratumoral microbiome signature that was highly predictive of the prognosis for HCC patients who underwent surgical resection. Specifically, the presence of Intestinimonas, Brachybacterium, and Rothia were identified as independent risk factors for the overall survival of HCC patients who underwent surgical resection.IMPORTANCEAlthough some studies have shown an abundance of bacteria in hepatocellular carcinoma (HCC), there is still limited understanding of the composition and diversity of the intratumoral microbiome that is favorable or adverse to the prognosis of HCC patients. Our results indicated that a greater abundance of bacteria could be observed in the neoplastic tissues than in nonneoplastic tissues. Bacterial cell wall components largely coincided with tumor-associated immune cells. The bacteria in the long overall survival (LOS) group were associated with metabolism and cytokine‒cytokine receptor interaction pathways, while bacteria in the short overall survival (SOS) group were associated with proinflammatory and cell proliferation pathways. Notably, specific taxa could independently predict HCC prognosis. Based on these findings, intratumoral microbiomes facilitate the use of precision medicine in clinical practice.https://journals.asm.org/doi/10.1128/msystems.00765-24intratumoral microbiomehepatocellular carcinoma16S rRNA gene sequencingbacterial componentstumor-associated immune cells |
| spellingShingle | Fengle Jiang Yuan Dang Zheting Zhang Yanan Yan Yingchao Wang Yi Chen Lihong Chen Jialiang Zhang Jingfeng Liu Jianmin Wang Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis mSystems intratumoral microbiome hepatocellular carcinoma 16S rRNA gene sequencing bacterial components tumor-associated immune cells |
| title | Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis |
| title_full | Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis |
| title_fullStr | Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis |
| title_full_unstemmed | Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis |
| title_short | Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis |
| title_sort | association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis |
| topic | intratumoral microbiome hepatocellular carcinoma 16S rRNA gene sequencing bacterial components tumor-associated immune cells |
| url | https://journals.asm.org/doi/10.1128/msystems.00765-24 |
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