Integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in Dendrobium nobile Lindl
Abstract Background Dendrobium nobile Lindl belongs to the genus Dendrobium of the orchid family and is a valuable herbal medicine. Drought stress severely affects the growth of D. nobile Lindl; however, the specific regulatory mechanisms have not yet been elucidated. Results In the present study, w...
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| Language: | English |
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BMC
2025-02-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06176-8 |
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| author | Chaoyan Lv Ya He Zaiqian Jiang Wenjia Hu Mei Zhang |
| author_facet | Chaoyan Lv Ya He Zaiqian Jiang Wenjia Hu Mei Zhang |
| author_sort | Chaoyan Lv |
| collection | DOAJ |
| description | Abstract Background Dendrobium nobile Lindl belongs to the genus Dendrobium of the orchid family and is a valuable herbal medicine. Drought stress severely affects the growth of D. nobile Lindl; however, the specific regulatory mechanisms have not yet been elucidated. Results In the present study, we conducted a combined transcriptome and metabolome analysis of D. nobile Lindl stems under different drought stress conditions. Global transcriptomic changes were detected in Dendrobium under different drought stress conditions. KEGG enrichment analysis showed that the DEGs were enriched in plant hormone signal transduction; cutin, suberin, and wax biosynthesis; starch and sucrose metabolism; and the biosynthesis of various plant secondary metabolites. The differentially abundant metabolites (DAMs) detected using STEM analysis were enriched in pathways associated with glucosinolate biosynthesis and cyanoamino acid metabolism. We constructed a regulatory network for the drought tolerance of Dendrobium by weighted gene co-expression analysis. Conclusions The results showed that arginine and proline metabolism, glucosinolate biosynthesis and tyrosine metabolism pathways participated in regulating drought stress in D. nobile Lindl. Our study provides a theoretical basis for studying the drought resistance mechanisms in Dendrobium. |
| format | Article |
| id | doaj-art-9837118cffca4c149efccb7d4fedc6c0 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-9837118cffca4c149efccb7d4fedc6c02025-02-09T12:27:52ZengBMCBMC Plant Biology1471-22292025-02-0125111310.1186/s12870-025-06176-8Integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in Dendrobium nobile LindlChaoyan Lv0Ya He1Zaiqian Jiang2Wenjia Hu3Mei Zhang4College of Biology and Agriculture, Zunyi Normal UniversityCollege of Biology and Agriculture, Zunyi Normal UniversityCollege of Biology and Agriculture, Zunyi Normal UniversityCollege of Biology and Agriculture, Zunyi Normal UniversityCollege of Biology and Agriculture, Zunyi Normal UniversityAbstract Background Dendrobium nobile Lindl belongs to the genus Dendrobium of the orchid family and is a valuable herbal medicine. Drought stress severely affects the growth of D. nobile Lindl; however, the specific regulatory mechanisms have not yet been elucidated. Results In the present study, we conducted a combined transcriptome and metabolome analysis of D. nobile Lindl stems under different drought stress conditions. Global transcriptomic changes were detected in Dendrobium under different drought stress conditions. KEGG enrichment analysis showed that the DEGs were enriched in plant hormone signal transduction; cutin, suberin, and wax biosynthesis; starch and sucrose metabolism; and the biosynthesis of various plant secondary metabolites. The differentially abundant metabolites (DAMs) detected using STEM analysis were enriched in pathways associated with glucosinolate biosynthesis and cyanoamino acid metabolism. We constructed a regulatory network for the drought tolerance of Dendrobium by weighted gene co-expression analysis. Conclusions The results showed that arginine and proline metabolism, glucosinolate biosynthesis and tyrosine metabolism pathways participated in regulating drought stress in D. nobile Lindl. Our study provides a theoretical basis for studying the drought resistance mechanisms in Dendrobium.https://doi.org/10.1186/s12870-025-06176-8Dendrobium nobile LindlDrought StressTranscriptomeMetabolomeRegulatory Network |
| spellingShingle | Chaoyan Lv Ya He Zaiqian Jiang Wenjia Hu Mei Zhang Integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in Dendrobium nobile Lindl BMC Plant Biology Dendrobium nobile Lindl Drought Stress Transcriptome Metabolome Regulatory Network |
| title | Integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in Dendrobium nobile Lindl |
| title_full | Integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in Dendrobium nobile Lindl |
| title_fullStr | Integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in Dendrobium nobile Lindl |
| title_full_unstemmed | Integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in Dendrobium nobile Lindl |
| title_short | Integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in Dendrobium nobile Lindl |
| title_sort | integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in dendrobium nobile lindl |
| topic | Dendrobium nobile Lindl Drought Stress Transcriptome Metabolome Regulatory Network |
| url | https://doi.org/10.1186/s12870-025-06176-8 |
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