Pupil responses to melanopsin-isolating stimuli as a potential diagnostic biomarker for glaucoma.
<h4>Purpose</h4>To test whether differences in pupil responses to melanopsin-isolating spectral stimuli in glaucoma may be useful as a diagnostic biomarker.<h4>Methods</h4>Spectral stimuli were presented to 20 glaucoma and 15 age-similar healthy control participants. Stimuli...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0324373 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <h4>Purpose</h4>To test whether differences in pupil responses to melanopsin-isolating spectral stimuli in glaucoma may be useful as a diagnostic biomarker.<h4>Methods</h4>Spectral stimuli were presented to 20 glaucoma and 15 age-similar healthy control participants. Stimuli were pairs of silent-substitution spectra designed to provide (1) equal stimulation to cone photoreceptors but maximum (~325%) contrast to melanopsin or (2) equal stimulation to melanopsin but ~325% contrast to cones. Narrowband long-wavelength/red (657 nm) and short-wavelength/blue (471 nm) pulses were also presented from a dark background to 16 glaucoma and 12 control participants. Pulses lasted 3 seconds and pupil size was measured for 15 seconds. Pupil response metrics were compared by t-test and relationships with visual field and OCT summary indices were assessed by Spearman's rank correlation. Diagnostic accuracy was measured by area under the receiver operating characteristic curve (AUC).<h4>Results</h4>Pupil constriction was more persistent after pulse offset for the melanopsin-directed stimulus (2% mean paired difference 6s post-pulse offset, p < 0.001). All pupil parameters were similar between groups (p = 0.04-0.90) for all stimuli. Correlations between pupil response parameters and visual field summary indices and circumpapillary retinal nerve fibre layer thickness were weak (rho 0.02-0.57, all p > 0.05). Diagnostic accuracy for all pupil parameters was poor, with AUC 95% confidence intervals overlapping 0.5 for all but time to maximal constriction for the cone-directed stimulus.<h4>Conclusions</h4>Pupil responses to melanopsin-isolating spectra were similar between glaucoma and control participants. Pupillary responses to melanopsin-isolating silent substitution spectra are unlikely to be useful as a diagnostic biomarker for glaucoma. |
|---|---|
| ISSN: | 1932-6203 |